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茶叶科学 ›› 2018, Vol. 38 ›› Issue (1): 87-93.doi: 10.13305/j.cnki.jts.2018.01.009

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围小丛壳菌山茶专化型与葡萄座腔菌复合侵染引致茶褐枯病

张永乐1, 刘会香1,*, 许永玉1, 何邦令1,*, 郑金柱2   

  1. 1. 山东农业大学植物保护学院/山东省林业有害生物防控工程技术研究中心, 山东 泰安 27018;
    2. 徂徕山林场,山东 泰安 271027
  • 收稿日期:2017-09-03 修回日期:2017-10-20 出版日期:2018-02-15 发布日期:2019-08-28
  • 通讯作者: *hxliu722@126.com,hebangling@126.com
  • 作者简介:张永乐,女,硕士,主要从事茶树病害病原鉴定及绿色防控技术研究。
  • 基金资助:
    山东省茶产业技术体系(SDAIT-19-04)

The Tea Brown Blight Disease Caused by Co-infection of Glomerella cingulata f. sp. camelliae and Botryosphaeria dothidea

ZHANG Yongle1, LIU Huixiang1,*, XU Yongyu1, HE Bangling1,*, ZHENG Jinzhu2   

  1. 1. Shangdong Agricultural University/Shandong Research Center for Forestry Harmful Biological Control Engineering and Technology, Taian 27018, China;
    2. Forestry Station of Culai Mountain, Tai′an 271027, China
  • Received:2017-09-03 Revised:2017-10-20 Online:2018-02-15 Published:2019-08-28

摘要: 在山东日照东港区茶园茶树叶部发现一种新病害。感病部位缢缩,畸形,病斑褐色,坏死,命名为茶褐枯病。取病健交界处采用组织分离法共获得两种微生物,编号为RC4、RC3,分离率分别为67.1%和32.9%。结合微生物形态学、培养学、多基因序列分析和柯赫氏法则验证,明确该病害由围小丛壳菌山茶专化型(Glomerella cingulata f. sp. camelliae)和葡萄座腔菌(Botryosphaeria dothidea)复合侵染引起。这也是首次在茶树叶部发现B. dothidea的危害。

关键词: 茶树, 葡萄座腔菌, 围小丛壳菌山茶专化型, 复合侵染, 鉴定

Abstract: A new leaf disease of tea plant was found in Donggang district of Rizhao, Shandong Province. The infected parts were shrunken and malformed, turned brown, necrotic and named brown blight of tea. Two microorganisms were isolated from the edge of infected parts through tissue separation, and named as RC4 and RC3. Their separation rates were 67.1% and 32.9% respectively. According to morphology, culture characteristics, multi- sequence analysis and Koch's Postulates test, Glomerella cingulata f. sp. camelliae and Botryosphaeria dothidea were found to be the cause of disease. This was the first report that Botryosphaeria dothidea damaged the leaves of tea plant.

Key words: tea plant, B. dothidea, G. cingulata f. sp. camelliae, co-infection, identify

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