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茶叶科学 ›› 2018, Vol. 38 ›› Issue (2): 162-173.doi: 10.13305/j.cnki.jts.2018.02.007

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茶树儿茶素合成相关MYB转录因子生物信息学分析

张玥, 胡雲飞, 王树茂, 柯子星, 林金科*   

  1. 福建农林大学安溪茶学院,福建 福州 350002
  • 收稿日期:2017-10-12 修回日期:2017-11-03 出版日期:2018-04-15 发布日期:2019-08-28
  • 通讯作者: *ljk213@163.com
  • 作者简介:张玥,女,讲师,博士研究生,主要从事茶树分子生物学研究,yaoyao86527@163.com。
  • 基金资助:
    福建省教育厅中青年教师教育科研项目(JAT170178)、福建农林大学人才引进科研启动基金(KXR15008A)、国家自然科学基金(31170651)

Bioinformatic Analysis of MYB Transcription Factors Involved in Catechins Biosynthesis in Tea Plant (Camellia sinensis)

ZHANG Yue, HU Yunfei, WANG Shumao, KE Zixing, LIN Jinke*   

  1. Anxi College of Tea Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China
  • Received:2017-10-12 Revised:2017-11-03 Online:2018-04-15 Published:2019-08-28

摘要: 从茶树(Camellia sinensis)转录组数据库中获得6个与儿茶素合成相关的MYB转录因子,对其进行蛋白理化性质与结构功能预测、核定位信号和蛋白保守结构域分析,通过同源分析构建系统发育树。结果表明,与儿茶素合成相关的6个MYB蛋白都属于亲水性的非分泌蛋白,定位在叶绿体、线粒体和细胞核上,其空间结构主要是α-螺旋和β-转角;保守结构域的分析发现除了comp159173_c0基因,其余5个都属于SANT超家族基因成员。系统进化树显示6个MYB蛋白形成4个分支,显示出各自间较远的亲缘关系。差异表达分析的结果进一步证实了6个MYB转录因子与儿茶素合成代谢的相关性。

关键词: 儿茶素, MYB转录因子, 生物信息学

Abstract: Six MYB transcription factors involved in the biosynthesis of catechins were obtained from the tea plant (Camellia sinensis) transcriptome database. Bioinformatic analysis including the physicochemical properties and structural functions of proteins, nuclear localization signal (NLS), protein conserved domain (PCD) and phylogenetic tree analysis were performed. The results demonstrated that the 6 MYB proteins involved in the biosynthesis of catechins belong to hydrophilic non-secretory proteins and are predicted to be located in the chloroplasts, mitochondria and nucleus. The main spatial structures are α-helixes and β-turns. Except the comp159173_c0, the other 5 genes belong to SANT super-family group based on the PCD analysis. The phylogenetic tree analysis shows that 6 MYB proteins are classified into 4 groups, indicating a distant relationship between them. Differential gene expression analysis further confirmed the close correlation between the 6 transcription factors and the catechin biosynthesis.

Key words: catechins, MYB transcription factors, bioinformatics

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