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茶叶科学 ›› 2018, Vol. 38 ›› Issue (3): 313-318.doi: 10.13305/j.cnki.jts.2018.03.012

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一株茶大灰象甲寄生真菌的分子鉴定及其毒力测定

王定锋, 李良德, 李慧玲, 张辉, 王庆森, 吴光远*   

  1. 福建省农业科学院茶叶研究所,福建 福安 355015
  • 收稿日期:2018-01-05 修回日期:2018-02-22 出版日期:2018-06-15 发布日期:2019-08-28
  • 通讯作者: *
  • 作者简介:王定锋,男,硕士,助理研究员,主要从事害虫生物防治研究。
  • 基金资助:
    福建省自然科学基金项目(2015J01099)、国家茶叶产业技术体系项目(CARS-19)、福建省农业科学院茶叶研究所重点项目(2014-cys-02)

Molecular Identification and Virulence Evaluation of an Entomopathogenic Fungus Isolated from the Cadaver of Sympiezomias citri

WANG Dingfeng, LI Liangde, LI Huiling, ZHANG Hui, WANG Qingsen, WU Guangyuan*   

  1. Tea Research Institute, Fujian Academy of Agricultural Sciences, Fu’an 355015, China
  • Received:2018-01-05 Revised:2018-02-22 Online:2018-06-15 Published:2019-08-28

摘要: 为明确分离自茶大灰象甲(Sympiezomias citri)自然羅病死亡成虫僵虫的一株寄生真菌Bbr4941菌株的具体分类归属及其生防潜力,对该菌株的rDNA-ITS序列和Bloc序列进行了PCR扩增、测序及系统进化树构建。结果明确了它为布氏白僵菌(Beauveria brongniartii)。在此基础上,利用浸虫法研究该菌株对茶大灰象甲成虫的室内毒力。结果表明,高浓度(每毫升5.0×107孢子)孢悬液处理时,第7天累计校正死亡率为100%,LT50为3.916βd。以上研究表明Bbr4941菌株对茶大灰象甲成虫具有很强的杀虫毒力,有望在今后茶大灰象甲的生物防治中发挥重要作用。

关键词: 茶大灰象甲, 昆虫病原真菌, rDNA-ITS, Bloc序列, 毒力

Abstract: The taxonomic status and biocontrol potential of an entomopathogenic fungus that isolated from the cadaver of Sympiezomias citri were investigated. Amplification, sequencing, and system evolution tree construction of the nuclear ribosomal internal transcribed spacer (rDNA-ITS) and intergenic Bloc region fragments (Bloc) of the fungus Bbr4941, and the bioassay against the adult of S. citri were carried out. The results showed that the entomopathogenic fungus was identified as Beauveria brongniartii. When the adults of S. citri were dealt with Bbr4941 suspension (5.0×107 spore·mL-1), the corrected accumulative mortality was 100% and LT50 value was 3.916βd after 7 days. Our results showed that Bbr4941 had great potential for the biocontrol of S. citri.

Key words: Sympiezomias citri, entomopathogenic fungi, rDNA-ITS, Bloc region, virulence

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