利用48对SSR引物对来自龙井群体种的5个不同居群的91份材料进行了遗传多样性和遗传分化研究。结果表明,龙井群体有较高的遗传多样性水平,多态信息含量PIC在0.0567~0.7476之间,平均为0.4382,其中有16个位点属于高度多态位点,30个位点属于中度多态位点。哈迪-温伯格法则（Hardy-Weinberg）平衡检验结果表明,33.3%的位点符合Hardy-Weinberg平衡,66.7%的SSR位点不符合Hardy-Weinberg平衡。AMOVA分析表明,不同群体之间所贡献的变异百分比为3.45%,个体间的变异百分比为94.98%。龙井群体的遗传分化程度较低,个体间遗传分化系数FIT为0.0502,群体之间的遗传分化系数FST仅为0.0345。
The genetic diversity and differentiation of 91 individuals from five populations of Longjing tea landrace were characterized by 48 pairs of SSR markers. It was showed that the level of genetic diversity within Longjing tea population was high. The polymorphism information content (PIC) was ranging from 0.0567 to 0.7476 and the average PIC was 0.4382. Among 48 SSR sites in study, 16 was high polymorphism, 30 was medium polymorphism. Hardy-Weinberg equiliberum (HWE) test showed that 33.3% SSR sites were in accord with the HWE and 66.7% sites were not in HWE. AMOVA analysis showed that percentage variation among populations was 3.45% and percentage variation within individuals was 94.98%. The genetic differentiation was lower in Longjing tea population, the fixation indices within individuals and among populations was 0.0502 (FIT) and 0.0345 (FST) respectively.
[1] 《中国茶树品种志》编写委员会. 中国茶树品种志[M]. 上海: 上海科学技术出版社, 2001.
[2] 李腊梅, 马军辉, 罗列万, 等. 龙井43在浙江省的推广及效益分析[J]. 茶叶, 2007, 33(1): 38-40.
[3] 沈程文. 茶树安化云台山种有性个体基因组差异的研究[D]. 长沙: 湖南农业大学, 2001: 18-28.
[4] 季鹏章, 张俊, 王平盛, 等. 云南古茶树(园)遗传多样性的ISSR分析[J]. 茶叶科学, 2007, 27(4): 271-279.
[5] Ohsako T, Ohgushi T, Motosugi H, et al. Microsatellite variability within and among local landrace populations of tea, Camellia sinensis (L.) O. Kuntze, in Kyoto, Japan[J]. Genet Resour Crop Evol, 2008(55):1047-1053.
[6] Powell W, Machray GC, Provan J.Polymorphism revealed by simple sequence repeats[J]. Trends in Plant Science, 1996(1): 215-222.
[7] 刘本英, 王平盛, 周红杰, 等. 云南茶组植物ISSR-PCR反应体系的建立[J]. 云南农业大学学报, 2006(增): 21-25.
[8] 王丽鸳, 姜燕华, 段云裳, 等. 茶树EST-SSRs分布特征及引物开发[J]. 植物遗传资源学报, 2009, 10(4) : 511-516.
[9] Jun-Bo Yang, Jing Yang, Hong-Tao Li, et al, Isolation and characterization of 15 microsatellite markers from wild tea plant (Camellia taliensis) using FIASCO method[J]. Conservation Genetics, 2009, 10(5): 1621-1623.
[10] 刘振, 王新超, 赵丽萍, 等.基于EST-SSR 的西南茶区茶树资源遗传多样性和亲缘关系分析[J]. 分子植物育种, 2008, 6(1): 100-110.
[11] Liu KJ, Muse SV.PowerMarker: Integrated analysis environment for genetic marker data[J]. Bioinformatics, 2005, 21(9): 2121-2129.
[12] Excoffier L, Laval L G, Schneider S.Arlequin ver. 3.0: An integrated software package for population genetics data analysis[J]. Evolutionary Bioinformatics Online, 2005(1): 47-50.
[13] Frankel O H, Hawkes J G.Crop Genetic Resources for Today and Tomorrow: Optimum Sampling Strategies in Genetic Conservation[M]. Cambridge: Cambridge University Press, 1975: 53-80.
[14]Botstein D, White RL, Skolnick M, et al. Construction of a genetic linkage map in man using restriction fragment length polymorphisms[J]. Am J Hun Genet, 1980, 32(3):314-331.
浙公网安备 33019902000101号 
