本文选取与糖脂代谢相关的PPARδ、PPARα、PPARγ、FXR、LXR、3T3-L1和α-淀粉酶模型,探讨了普洱茶中单体功能成分尿嘧啶、没食子酸的降糖降脂作用。结果表明：尿嘧啶、没食子酸在PPARγ、FXR、LXR模型表现出明显的活性作用,其中尤以没食子酸对PPARγ的激活效果最为显著,其值高达2.438,与阳性对照药物的激动效果相当,而对PPARδ、PPARα、3T3-L1模型的活性作用较弱。在PPARγ模型中,没食子酸对PPARγ受体的激活作用强于尿嘧啶,而在FXR、LXR模型中尿嘧啶的活性作用要强于没食子酸。此外,没食子酸对α-淀粉酶也有较强的抑制作用。本研究结果可为普洱茶的降糖降脂作用机理提供一定的理论依据。
The anti-diabetes and anti-hyperlipidenmia function of Uracil and Gallic acid from Pu-erh Tea was studied by the PPARδ, PPARα, PPARγ, FXR, LXR, 3T3-L1 and α-amylase models which are related to glucose and lipid metabolism. Result showed that: Uracil and gallic acid were active to the models of PPARγ, FXR, LXR, especially, Gallic acid showed distinct active effect on PPARγ, the value was as high as 2.438 which showed the same effect as positive drug, and weak activity on PPARδ, PPARα, 3T3-L1 models. Gallic acid showed better activity than Uracil in the PPARγ model and Uracil showed better activity than gallic acid in the FXR and LXR models. Furthermore, Gallic acid showed strongth inhibition on the activity of α-amylase. It can provide some theoretical basis on the mechanism of Pu-erh tea on the anti-diabetes and anti-hyperlipidenmia activity.
[1] 周志宏, 杨崇仁. 云南普洱茶原料晒青毛茶的化学成分[J]. 云南植物研究, 2000, 22(3): 343~350.
[2] 吕海鹏. 普洱茶化学成分与抗氧化活性研究[M]. 杭州: 中国农业科学院硕士研究生论文, 2005.
[3] 张冬英, 刘仲华, 刘亚林, 等. 高通量筛选法对普洱茶降血糖降血脂作用研究[J]. 茶叶科学, 2006, 26(1): 49~53.
[4] 傅冬和, 刘仲华, 黄建安, 等. 高通量筛选研究茯砖茶对FXR模型的作用[J]. 食品科学, 2007, 28(5): 331~334.
[5] 傅冬和, 刘仲华, 黄建安, 等. 茯砖茶不同萃取物对消化酶活性的影响[J]. 茶叶科学, 2008, 28(1): 62~66.
[6] 张冬英. 普洱茶降糖降脂活性成分研究[M]. 长沙: 湖南农业大学博士研究生论文, 2006.
[7] Zhang Y, Repa JJ, Gauthier K, et al. Regulation of lipoprotein lipase by the oxysterol reseptors, LXRalpha and LXRbeta[J]. J Biol Chem, 2001, 276(46): 43018~43024.
[8] Urizar NL, Dowhan DH, Moore DD.The farnesoid X-activated receptor mediates bile acid activation of phospholipid transfer protein gene expression[J]. J Biol Chem, 2000, 275(50): 39313~39317.
[9] Lee C H, Chawla A, Urbiztondo N, et a1.Transcriptional repression of atherogenic inflammation: modulation by PPAR-delta[J]. Science, 2003, 302: 455~457.
[10] Frohnet B I, Hui T Y, Bernlohr DA.Identification Of functional peroxisome proliferators-responsive element in murine fatty acid transport protein gene[J]. J Biol Chem, 1999, 274: 3970~3977.
[11] Schultz JR, Tu H, Luk A, et a1. Role of LXRs in control of lipogenesis[J]. Genes Dev, 2000, 14: 2831-2838.
[12] Urizar N L, Liveryman A B, Dodd’s DT, et a1. A natural product that lowers cholesterol as antagonist ligand for FXR[J]. Science, 2002, 296: 1703~1706.
[13] 李肖玲, 崔岚, 祝德秋. 没食子酸生物学作用的研究进展[J]. 中国药师, 2004, 7(10): 767~769.
浙公网安备 33019902000101号 
