茶树根际土壤真菌ALF-1（Neurospora sp.）耐酸铝基因片段克隆

陆建良; 梁月荣; 吴颖; 黄福平

doi:10.13305/j.cnki.jts.2004.01.008

茶叶科学 >

2004 , Vol. 24 >Issue 1: 41 - 43

DOI: https://doi.org/10.13305/j.cnki.jts.2004.01.008

茶树根际土壤真菌ALF-1（Neurospora sp.）耐酸铝基因片段克隆

陆建良 ,
梁月荣 ,
吴颖 ,
黄福平

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浙江大学茶叶研究所,浙江杭州 310029

陆建良（1970— ），男，浙江平湖人，浙江大学副教授，主要从事茶树生物技术与资源利用方面的研究。

收稿日期: 2003-10-24

修回日期: 2004-02-12

网络出版日期: 2019-09-11

基金资助

博士点基金项目2000033539和国家基金项目30170591

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Cloning of Aluminum-tolerant Associated Gene from Fungus ALF-1(Neurospora sp.) in Rhizospheric Soil of Tea Plant

LU Jian-liang ,
LIANG Yue-rong ,
WU Ying ,
HUANG Fu-ping

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Tea Research Institute of Zhejiang University, Hangzhou 310029, China

Received date: 2003-10-24

Revised date: 2004-02-12

Online published: 2019-09-11

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摘要

采用rt-PCR克隆了茶树根际土壤耐酸铝真菌ALF-1（Neurospora sp.）与耐酸铝相关的基因片段,该片段由ALF-1在酸铝诱导条件才表达。序列分析后获得359βbp的可读片段,最大开放阅读框为260βbp,位于第100-359βbp之间,编码86个氨基酸残基。BLAST分析结果表明,该片段与Arabidopsis thaliana (thale cress)的耐酸铝相关基因序列同源性为50%。

关键词： 耐酸铝真菌; 耐酸铝基因; 克隆

本文引用格式

陆建良 , 梁月荣 , 吴颖 , 黄福平 . 茶树根际土壤真菌ALF-1（Neurospora sp.）耐酸铝基因片段克隆[J]. 茶叶科学, 2004 , 24(1) : 41 -43 . DOI: 10.13305/j.cnki.jts.2004.01.008

Abstract

Aluminum-tolerant associated gene of fungus ALF-1(Neurospora sp.) in rhizospheric soil of tea plant was cloned using rt-PCR technique. The gene expression was induced by aluminum treatment, and its sequence was 359 base pair (bp). The opening reading frame (ORF) was 260bp which situated from 100^th to 359^thbp, and encoded 86 amino acid residues. BLAST analysis showed that the gene sequence obtained was 50% identity to that of Arabidopsis thaliana (thale cress).

Key words： Aluminum-tolerant fungus; Aluminum-tolerant gene; Cloning

参考文献

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[7] 梁月荣,刘祖生,陆建良,等. 茶树根际土壤抗酸铝真菌ALF-1(Neurospora sp.)对酸性土壤pH的影响[J]. 茶叶科学,1999,19(2):115-118.
[8] 梁月荣,刘祖生,陆建良,等. 茶树根际土壤抗酸铝真菌ALF-1(Nerospora sp.)抗酸铝机理[J]. 茶叶科学,1999,19(2):119-124.
[9] Lu Jianliang, Liang Yuerong.http://www.ncbi.nlm.nih.gov/, accession number: AY364443.

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