Preparation of Gallic Acid Derivatives by Solid-phase Extraction Cartridge Coupled with Preparative High Performance Liquid Chromatography from Tea Plant

LU Zhong-wei; JIANG Xiao-lan; LIU Ya-jun; LIU Li; GAO Li-ping; XIA Tao

doi:10.13305/j.cnki.jts.2012.06.001

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Journal of Tea Science ›› 2012, Vol. 32 ›› Issue (6) : 494-499. DOI: 10.13305/j.cnki.jts.2012.06.001

Preparation of Gallic Acid Derivatives by Solid-phase Extraction Cartridge Coupled with Preparative High Performance Liquid Chromatography from Tea Plant

LU Zhong-wei¹, JIANG Xiao-lan², LIU Ya-jun¹, LIU Li², GAO Li-ping^1*, XIA Tao^2*

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Abstract

Gallic acid derivatives are the chief compounds of phenolic acid in tea. They are important umami-enhancing compounds in tea beverage, and possess the effects of inhibiting IgE producton, enhancing cognition-anting depressant. A reversed-phase PHPLC coupled with SPE was developed to purify and to prepare high purity 3-O-galloyquinic acid and β-D-glucogalline from fresh tea leaves. The obtained fractions were successfully identified by LC/TOF-ESI-MS, ¹H-NMR and ¹³C-NMR. This method is simple, effective for the preparation of gallic acid derivatives.

Key words

β-D-glucogalline / 3-O-galloyquinic acid / gallic acid derivatives / PHPLC / SPE

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LU Zhong-wei, JIANG Xiao-lan, LIU Ya-jun, LIU Li, GAO Li-ping, XIA Tao. Preparation of Gallic Acid Derivatives by Solid-phase Extraction Cartridge Coupled with Preparative High Performance Liquid Chromatography from Tea Plant[J]. Journal of Tea Science. 2012, 32(6): 494-499 https://doi.org/10.13305/j.cnki.jts.2012.06.001

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