Welcome to Journal of Tea Science,Today is

Analysis of Differential Gene Expression on Flavonoid Biosynthesis and Its Regulation in Tea Plant (Camellia sinensis L.)

  • WEI Kang ,
  • WANG Liyuan ,
  • CHENG Hao ,
  • ZHANG Liqun ,
  • FENG Suhua
Expand
  • Tea Research Institute, Chinese Academy of Agriculture Science, National Center for Tea Improvement, Hangzhou 310008, China

Received date: 2013-07-01

  Revised date: 2013-08-12

  Online published: 2019-09-03

Abstract

The expression of genes associated with flavonoid biosynthesis and its regulation in petal, pollen, dormant and sprouting buds of tea plants were analyzed by differential gene expression. The results showed that flavonoid biosynthetic genes were highly expressed in petal, dormant and sprouting buds, but rarely expressed in pollen, which is consistent with the distribution of flavonoids in tea plant. Furthermore, twelve genes in MYB, bHLH, MADS, GST, WD40 and Homeodomain gene families were identified to be correlated with flavonoid biosynthesis in petal. Nine genes were found to be involved in the regulation of flavonoid biosynthesis in tea buds. These results will be a basis for further understanding the mechanism of flavonoid biosynthetsis and regulation in Camellia sinensis.

Cite this article

WEI Kang , WANG Liyuan , CHENG Hao , ZHANG Liqun , FENG Suhua . Analysis of Differential Gene Expression on Flavonoid Biosynthesis and Its Regulation in Tea Plant (Camellia sinensis L.)[J]. Journal of Tea Science, 2014 , 34(2) : 195 -203 . DOI: 10.13305/j.cnki.jts.2014.02.016

References

[1] Cabrera C, Artacho R, Giménez R.Beneficial effects of green tea-a review[J]. Journal of the American College of Nutrition, 2006, 25(2): 79-99.
[2] 乔小燕, 马春雷, 陈亮. 植物类黄酮生物合成途径及其重要基因的调控[J]. 天然产物研究与开发, 2009, 21(2): 354-360.
[3] 夏涛, 高丽萍. 类黄酮及茶儿茶素生物合成途径及其调控研究进展[J]. 中国农业科学, 2009, 42(8): 2899-2908.
[4] Broun P.Transcriptional control of flavonoid biosynthesis: a complex network of conserved regulators involved in multiple aspects of differentiation in Arabidopsis[J]. Current Opinion in Plant Biology, 2005, 8(3): 272-279.
[5] Dixon RA, Xie DY, Sharma SB.Proanthocyanidins-a final frontier in flavonoid research?[J] New Phytologist, 2005, 165(1): 9-28.
[6] 王丽鸳. 基于EST数据库和转录组测序的茶树DNA分子标记开发与应用研究[D]. 北京: 中国农业科学院研究生院,2011: 55-82.
[7] 韦康, 王丽鸳, 成浩, 等. 温湿度变化对茶苗芽萌发基因表达的影响[J]. 茶叶科学, 2013, 33(2): 109-115.
[8] Morrissy AS, Morin RD, Delaney A.Next-generation tag sequencing for cancer gene expression profiling[J]. Genome Research, 2009, 19(10): 1825-1835.
[9] Ulusoy E, Kolayli. Phenolic composition and antioxidant properties of Anzer bee pollen [J]. Journal of Food Biochemistry, 2013(DOI: 10.1111/jfbc.12027).
[10] Baudry A, Caboche M, Lepiniec L.TT8 controls its own expression in a feedback regulation involving TTG1 and homologous MYB and bHLH factors, allowing a strong and cell-specific accumulation of flavonoids in Arabidopsis thaliana[J]. The Plant Journal, 2006, 46(5): 768-779.
[11] Dixon DP, Lapthorn A, Edwards R. Plant glutathione transferases [J]. Genome Biology, 2002, 3(3): 3004.1-3004.10.
[12] Punyasiri PAN, Abeysinghe ISB, Kumar V, et al. Flavonoid biosynthesis in the tea plant Camellia sinensis: properties of enzymes of the prominent epicatechin and catechin pathways[J]. Archives of Biochemistry and Biophysics, 2004, 431(1): 22-30.
[13] 马春雷. 茶树查尔酮异构酶、黄酮醇合成酶和无色花色素还原酶等基因的克隆与表达分析[M]. 北京: 中国农业科学院研究生院,2007: 25-57.
[14] Wei K, Wang L, Zhou J, et al. Catechin contents in tea (Camellia sinensis) as affected by cultivar and environment and their relation to chlorophyll contents[J]. Food Chemistry, 2011, 125(1): 44-48.
Outlines

/