EGCG3"Me could be produced from EGCG catalyzed by EGCG-O-Methyltransferase. Taken the yield of EGCG3"Me as main index, the present study focused on the producing conditions of EGCG-O-Methyltransferase induced by IPTG in recombinant E. coli bacteria. Results showed that the optimum producing conditions were as follows: the concentration of IPTG was 0.05mmol/L, the induction time was 20h, the initial pH of medium was 7.0 and the induction temperature was 20℃.
FEI Dong-mei
,
LIN Zhi
,
LU Hai-peng
,
ZHANG Yue
,
TAN Jun-feng
,
GUO Li
. Study on Expression Conditions of EGCG-O-Methyltransferase in Recombinant Escherichia coli Bacteria[J]. Journal of Tea Science, 2011
, 31(4)
: 333
-340
.
DOI: 10.13305/j.cnki.jts.2011.04.001
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