Tea plant is a fluoride hyperaccumulator with high fluoride in leaves, which has many health benefits to human body. Tea pectin acetylesterase (PAE) may be related to fluoride accumulation and detoxification by regulating pectin deacytelation. However, few reports on tea PAEs were available. In the study, the identification and analysis of characteristic, evolutionary and subcellular localization of CsPAEs were performed by bioinformatics method based on tea genome database and three next-generation sequencing data of Camellia sinensis var. sinensis cv ‘Shuchazao’. The results indicate that 12 CsPAEs belonged to five members, including PAE5, PAE8, PAE9, PAE10 and PAE12. Nine conserved motifs including CLDG, PxYH, GGGWC, GS, NWN, rYCDg, GCSAG, NaAYDSWQ and HCQ were found in CsPAEs; CsPAEs were more closely related to PAEs of Vitis vinifera and Theobroma cacao according to evolutionary relationship; Amino acid length, molecular weight and theoretical isoelectric point of 12 CsPAEs varies from 326-515, 36.7-56.9βkDa and 5.1-8.9, respectively. Except that CsPAE5 and CsPAE5-1 were predicated to be localized in the mitochondrion, and CsPAE10-1 might be present in cellular martix. The rest CsPAEs were predicated to be localized in the cell wall. CsPAE5, CsPAE5-1, CsPAE12 and CsPAE12-1 were transmembrane proteins. The results provided a foundation for functional analysis of CsPAEs involved in fluoride accumulation and detoxification.
LIU Yanli
,
MA Linlong
,
CAO Dan
,
JIN Xiaofang
,
FENG Lin
,
GONG Ziming
. Identification and Bioinformatic Analysis of Pectin Acetylesterases from Tea Plant[J]. Journal of Tea Science, 2019
, 39(5)
: 521
-529
.
DOI: 10.13305/j.cnki.jts.2019.05.003
[1] Roberts K.How the cell wall acquired a cellular context[J]. Plant Physiology, 2001, 125(1): 127-130.
[2] Mohnen D.Pectin structure and biosynthesis[J]. Current Ppinion in Plant Biology, 2008, 11(3): 266-277.
[3] Caffall K H, Mohnen D.The structure, function, and biosynthesis of plant cell wall pectic polysaccharides[J]. Carbohydrate Research, 2009, 344(14): 1879-1900.
[4] Krzesłowska M.The cell wall in plant cell response to trace metals: polysaccharide remodeling and its role in defense strategy[J]. Acta Physiologiae Plantarum, 2011, 33(1): 35-51.
[5] Philippe F, Pelloux J, Rayon C.Plant pectin acetylesterase structure and function: new insights from bioinformatics analysis[J]. BMC Genomics, 2017, 18(1): 456. DOI: 10.1186/s12864-017-3833-0.
[6] Sengkhamparn N, Bakx E J, Verhoef R, et al.Okra pectin contains an unusual substitution of its rhamnosyl residues with acetyl and alpha-linked galactosyl groups[J]. Carbohydrate Research 2009, 344(14): 1842-1851.
[7] Buffetto F, Ropartz D, Zhang X J, et al.Recovery and fine structure variability of RGII sub-domains in wine (Vitis vinifera Merlot)[J]. Annals of Botany, 2014, 114(6): 1327-1337.
[8] Scheller H V, Ulvskov P.Hemicelluloses[J]. Annual Review of Plant Biology, 2010, 61: 263-289.
[9] Gou J Y, Miller L M, Hou G C, et al.Acetylesterase-mediated deacetylation of pectin impairs cell elongation, pollen germination, and plant reproduction[J]. The Plant Cell, 2012(24): 50-65.
[10] Bonnin E, Clavurier K, Daniel S, et al.Pectin acetylesterases from Aspergillus are able to deacetylate homogalacturonan as well as rhamnogalacturonan[J]. Carbohydrate Polymers, 2008, 74(3): 411-418.
[11] Orfila C, Dal Degan F, Jørgensen B, et al.Expression of mung bean pectin acetylesterase in potato tubers: effect on acetylation of cell wall polymers and tuber mechanical properties[J]. Planta, 2012, 236(1): 185-196.
[12] Matas A J, Rodríquez, V, Sánchez L, et al. Down-regulation of a pectin acetylesterase gene modifies strawberry fruit cell wall pectin structure and increases fruit firmness[J]. Current Research in Plant Physiology (XIV Congreso Hispano-Luso de Fisiología Vegetal), 2015, 1(1): 320.
[13] Vercauteren I, de Almeida Engler J, De Groodt R, et al. An Arabidopsis thaliana pectin acetylesterase gene is upregulated in nematode feeding sites induced by root-knot and cyst nematodes[J]. Moleculae Plant-Microbe Interactions, 2002, 15(4): 404-407.
[14] Pogorelko G, Lionetti V, Fursova O, et al.Arabidopsis and Brachypodium distachyon transgenic plants expressing Aspergillus nidulans acetylesterases have decreased degree of polysaccharide acetylation and increased resistance to pathogens[J]. Plant Physiology, 2013, 162(1): 9-23.
[15] 杨志远, 杨钢, 曹华, 等. 果胶乙酰酯酶基因AtPae7参与拟南芥对大豆疫霉菌的非寄主抗病性[J]. 西北农业学报, 2016, 25(11): 1716-1722.
[16] Liu Y L, Cao D, Ma L L, et al.TMT-based quantitative proteomics analysis reveals the response of Camellia sinensis to fluoride[J]. Journal of proteomics, 2018, 176: 71-81.
[1] Souza A J D, Pauly M. Comparative genomics of pectinacetylesterases: insight on function and biology[J]. Plant Signaling & Behavior, 2015, 10(9): e1055434. DOI: 10.1080/15592324.2015.1055434.
[17] Xia E H, Zhang H B, Sheng J, et al.The tea tree genome provides insights into tea flavor and independent evolution of caffeine biosynthesis[J]. Molecular Plant, 2017, 10(6): 866-877.
[18] Wei C L, Yang H, Wang S B, et al.Draft genome sequence of Camellia sinensis var. sinensis provides insights into the evolution of the tea genome and tea quality[J]. Proceedings of the National Academy of Sciences of the United States of America, 2018, 115(18): E4151-E4151.
[19] Cao D, Liu Y L, Ma L L, et al.Transcriptome analysis of differentially expressed genes involved in selenium accumulation in tea plant (Camellia sinensis)[J]. PLoS ONE, 2018, 13(6): e0197506. DOI: 10.1371/journal.pone.0197506.
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