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Journal of Tea Science ›› 2009, Vol. 29 ›› Issue (5): 341-346.doi: 10.13305/j.cnki.jts.2009.5.003

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Phylogenetic Analysis of Interspecies in Section Thea Through SSR Markers

WANG Li-yuan1, LIU Ben-ying1, JIANG Yan-hua1, DUAN Yun-shang2, CHENG Hao1,*, ZHOU Jian1, TANG Yi-chun3   

  1. 1. Tea Research Institute, Chinese Academy of Agricultural Sciences; National Center for Tea Improvement, Hangzhou 310008, China;
    2. Nanjing Agricultural University; Nanjing 210095, China;
    3.Tea Research Institute of Yunnan Academy of Agricultural Sciences, Menghai 666201, China
  • Received:2009-04-08 Revised:2009-06-09 Published:2019-09-09

Abstract: For the purpose of investigating the transferability and polymorphism information content (PIC) of different types of SSR primers, PCR amplification for 41 accessions from section thea was conducted in this investigation. The results showed that the SSR primers form Ser. Sinensis were highly transferable among section Thea. The polymorphic and transferable primers account for 60% of EST-SSR markers. Then the genetic diversity among 12 species of section Thea was evaluated through SSR analysis. The number of alleles in different loci ranged from 2 to 6, with average 4.21 per locus. The phyolgenetic dendrogram was constructed by the UPGMA.

Key words: section Thea, phylogenetic analysis, SSR marker, transferability

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