Phylogenetic Analysis of Interspecies in Section Thea Through SSR Markers

WANG Li-yuan; LIU Ben-ying; JIANG Yan-hua; DUAN Yun-shang; CHENG Hao; ZHOU Jian; TANG Yi-chun

doi:10.13305/j.cnki.jts.2009.5.003

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Journal of Tea Science ›› 2009, Vol. 29 ›› Issue (5) : 341-346. DOI: 10.13305/j.cnki.jts.2009.5.003

Phylogenetic Analysis of Interspecies in Section Thea Through SSR Markers

WANG Li-yuan¹, LIU Ben-ying¹, JIANG Yan-hua¹, DUAN Yun-shang², CHENG Hao^1,*, ZHOU Jian¹, TANG Yi-chun³

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Abstract

For the purpose of investigating the transferability and polymorphism information content (PIC) of different types of SSR primers, PCR amplification for 41 accessions from section thea was conducted in this investigation. The results showed that the SSR primers form Ser. Sinensis were highly transferable among section Thea. The polymorphic and transferable primers account for 60% of EST-SSR markers. Then the genetic diversity among 12 species of section Thea was evaluated through SSR analysis. The number of alleles in different loci ranged from 2 to 6, with average 4.21 per locus. The phyolgenetic dendrogram was constructed by the UPGMA.

Key words

phylogenetic analysis / section Thea / SSR marker / transferability

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WANG Li-yuan, LIU Ben-ying, JIANG Yan-hua, DUAN Yun-shang, CHENG Hao, ZHOU Jian, TANG Yi-chun. Phylogenetic Analysis of Interspecies in Section Thea Through SSR Markers[J]. Journal of Tea Science. 2009, 29(5): 341-346 https://doi.org/10.13305/j.cnki.jts.2009.5.003

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