Cloning and Sequence Analysis of EoblGOBP in the Antennal of Ectropis obliqua hypulina

CHEN Hua-cai; LIU Jun; ZHANG Xiao-yan; YIN Kun-shan

doi:10.13305/j.cnki.jts.2010.01.006

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Journal of Tea Science ›› 2010, Vol. 30 ›› Issue (1) : 37-44. DOI: 10.13305/j.cnki.jts.2010.01.006

Cloning and Sequence Analysis of EoblGOBP in the Antennal of Ectropis obliqua hypulina

CHEN Hua-cai¹, LIU Jun¹, ZHANG Xiao-yan¹, YIN Kun-shan²

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Abstract

The full-length sequences of two general odorant binding protein (GOBP) genes in Ectropis obliqua (named EoblGOBP1 and EoblGOBP2) were cloned with degenerated digonudeotide primers by RT-PCR and the rapid amplification of cDNA ends (RACE) using the total RNA isolated from the antenna of Ectropis obliqua as template. The length of EoblGOBP1 was 1 528 bp with a 501bp ORF which encoded a peptide of 166 amino acid residues. The predicted Mw and pI of the peptide were 18 835.63 D and 5.45 respectively. The NCBI/GenBank accession numbers of cDNA and amino acid of EoblGOBP1were FJ156732 and ACN29680.1, respectively. The length of EoblGOBP2 was 1 315 bp with a 405 bp ORF which encoded a peptide of 160 amino acid residues. The predicted Mw and pI of the peptide were 18 002.75 D and 5.32 respectively. The GenBank accession numbers of cDNA and amino acid of EoblGOBP2 were FJ 156733 and ACN29681.1, respectively. The amino acid sequences from the two gene sequences had 6 typical conservative Cys. Blast searching in NCBI showed that the amino acid sequences were highly similar to that of other GOBPs from Lepidoptera with sequence identities of 62%~82% and 76%~88%.

Key words

Ectropis oblique / full-length sequence / gene cloning / general odorant binding protein / sequence analysis

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CHEN Hua-cai, LIU Jun, ZHANG Xiao-yan, YIN Kun-shan. Cloning and Sequence Analysis of EoblGOBP in the Antennal of Ectropis obliqua hypulina[J]. Journal of Tea Science. 2010, 30(1): 37-44 https://doi.org/10.13305/j.cnki.jts.2010.01.006

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