Gene Cloning and Expression Analysis of GAGP in Tea Plant

LI Yuanhua; LU Jianliang; FAN Fangyuan; SHI Yutao

doi:10.13305/j.cnki.jts.2015.01.012

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Journal of Tea Science ›› 2015, Vol. 35 ›› Issue (1) : 64-72. DOI: 10.13305/j.cnki.jts.2015.01.012

Gene Cloning and Expression Analysis of GAGP in Tea Plant

LI Yuanhua¹, LU Jianliang², FAN Fangyuan², SHI Yutao¹

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Abstract

By using SSH, the differences in gene expression of root from Fudingdabai tea plant infected by VA mycorrhiza were analyzed and the diversity sequences was obtained. The sequence alighment showed that the down-regulated expression sequence possibly contained 10 unknown genes and the up-regulated expression sequence possibly contained 5 known genes. The GAGP (gap-pol) genic full-length sequence was obtained by using RACE. The length of GAGP gene was 3146bp (GenBank, Accession no., KJ946251), with 2β769βbp ORF (1^st-2β769^th), the sequence encoded 923 amino acid. Bioinformatics indicated that the GAGP protein’s molecular weight was about 106.9βkD, IEP was 8.42, located in mitochondria. The study also showed expression degree of GAGP was distinct in different cultivars, while it responded obviously to biological and non-biological stress.

Key words

expression / GAGP / gene clone / tea plant

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LI Yuanhua, LU Jianliang, FAN Fangyuan, SHI Yutao. Gene Cloning and Expression Analysis of GAGP in Tea Plant[J]. Journal of Tea Science. 2015, 35(1): 64-72 https://doi.org/10.13305/j.cnki.jts.2015.01.012

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