Welcome to Journal of Tea Science,Today is

Journal of Tea Science ›› 2015, Vol. 35 ›› Issue (1): 64-72.doi: 10.13305/j.cnki.jts.2015.01.012

Previous Articles     Next Articles

Gene Cloning and Expression Analysis of GAGP in Tea Plant

LI Yuanhua1, LU Jianliang2, FAN Fangyuan2, SHI Yutao1   

  1. 1. College of Tea and Food Science, Wuyi University, Wuyishan 354300, China;
    2. Tea Research Institute of Zhejiang University, Hangzhou 310058, China
  • Received:2014-09-09 Revised:2014-10-13 Published:2019-08-23

Abstract: By using SSH, the differences in gene expression of root from Fudingdabai tea plant infected by VA mycorrhiza were analyzed and the diversity sequences was obtained. The sequence alighment showed that the down-regulated expression sequence possibly contained 10 unknown genes and the up-regulated expression sequence possibly contained 5 known genes. The GAGP (gap-pol) genic full-length sequence was obtained by using RACE. The length of GAGP gene was 3146bp (GenBank, Accession no., KJ946251), with 2β769βbp ORF (1st-2β769th), the sequence encoded 923 amino acid. Bioinformatics indicated that the GAGP protein’s molecular weight was about 106.9βkD, IEP was 8.42, located in mitochondria. The study also showed expression degree of GAGP was distinct in different cultivars, while it responded obviously to biological and non-biological stress.

Key words: tea plant, GAGP, gene clone, expression

CLC Number: