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Journal of Tea Science ›› 2015, Vol. 35 ›› Issue (1): 97-102.doi: 10.13305/j.cnki.jts.2015.01.018

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Screeining and Identification of the Endophytic Bacterial Strain Against Ice Nucleation Active Bacteria of Tea Plant

HUANG Xiaoqin1,2, ZHANG Lixia1,2, LIU Huixiang3, CHEN Zongmao4, LI Duochuan3, *   

  1. 1. College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an 271018, China;
    2. Shandong Key Laboratory of Crop Biology, Tai’an 271018, China;
    3. Department of Plant Pathology, Shandong Agricultural University, Tai’an 271018, China;
    4. Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China
  • Received:2014-08-12 Revised:2014-12-15 Published:2019-08-23

Abstract: In order to protect the tea plant against freezing injury, the screening of antagonistic bacteria against INA bacteria No. 8 (Pantoea ananatis) and No. 14 (P. agglomerans) isolated from tea plant was carried out in Shandong Province. The strain Y1,an antagonistic bacteria against INA bacteria was isolated from the endophytic bacteria strains of tea plant. Based on the morphological characteristics and 16βS rDNA sequence analysis, the strain Y1 was identified as the Bacillus amyloliquefaciens. Through this study, the endophytic bacteria strain against the ice nucleation bacteria was obtained and identefied, it will be beneficial to the development of the biological control of ice nucleation bacterial.

Key words: tea plant, ice nucleation active bacteria, antagonistic bacterial strain, endophytic strains, screening, identification

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