SSR Identification and Pedigree Analysis of PVP Application Cultivars in Tea Plant

HUANG Danjuan; MA Jianqiang; CHEN Liang

doi:10.13305/j.cnki.jts.2016.01.009

PDF(1433 KB)

Journal of Tea Science ›› 2016, Vol. 36 ›› Issue (1) : 68-76. DOI: 10.13305/j.cnki.jts.2016.01.009

SSR Identification and Pedigree Analysis of PVP Application Cultivars in Tea Plant

HUANG Danjuan, MA Jianqiang, CHEN Liang^*

Author information +

History +

Abstract

Thirty SSR markers were used in this study for molecular identification and pedigree analysis of 26 PVP application tea cultivars, and 13 similar cultivars and parents, for probing the possible application of PVP and DUS testing using SSR markers in tea plant. A total of 131 alleles were detected, the number of alleles detected by each SSR marker ranged from 3 to 7, with a mean of 4.4. The average values of Shannon index and polymorphism information content were 1.04 and 0.51, respectively. The genetic distance ranged from 0.03 to 0.70 between 39 cultivars. When the genetic distance was 0.15, they could be classified into 7 groups. Tea cultivars from the same province and genetic background were clustered into one group to some extent. The identification ability of the 30 SSR markers was quite different, each marker could identify 3-16 cultivars. The 39 tea cultivars could be clearly distinguished by 4 core primers which were used to construct the molecular fingerprinting of all cultivars.

Key words

fingerprinting / PVP / SSR markers / tea cultivars

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

HUANG Danjuan, MA Jianqiang, CHEN Liang. SSR Identification and Pedigree Analysis of PVP Application Cultivars in Tea Plant[J]. Journal of Tea Science. 2016, 36(1): 68-76 https://doi.org/10.13305/j.cnki.jts.2016.01.009

References

[1] 黄丹娟,马建强,陈亮. 茶树DNA分子指纹图谱研究进展[J]. 茶叶科学, 2015, 35(6): 513-519.
[2] 吴渝生, 杨文鹏, 郑用琏. 3个玉米杂交种和亲本SSR指纹图谱的构建[J]. 作物学报, 2003, 29(4): 496-500.
[3] 张金渝, 张建华, 杨晓洪, 等. 玉米DUS 测试标准品种的SSR分子指纹图谱的构建[J]. 玉米科学, 2006, 14(4): 47-52.
[4] 李根英, Dreisigacker S, Warburton M L, 等. 小麦指纹图谱数据库的建立及SSR分子标记试剂盒的研发[J]. 作物学报, 2006, 32(12): 1771-1778.
[5] 张晗, 姚凤霞, 刘永杰, 等. EST-SSR 标记在冬小麦品种DUS 测试中的应用[J]. 麦类作物学报, 2010, 30(5): 801-806.
[6] 陆徐忠, 倪金龙, 李莉, 等. 利用SSR分子指纹和商品信息构建水稻品种身份证[J]. 作物学报, 2014, 40(5): 823-829.
[7] 唐浩, 余汉勇, 张新明, 等. 水稻新品种测试的标准品种DNA指纹图谱多样性分析[J]. 植物遗传资源学报, 2015, 16(1): 100-106.
[8] Dellaporta S L, Wood J, Hicks J B.A plant DNA mini preparation: version II[J]. Plant molecular biology reporter, 1983(1): 19-21.
[9] 马建强. 茶树高密度遗传图谱的构建及重要性状QTL定位[D]. 北京: 中国农业科学院, 2013: 88-104.
[10] Charters Y M, Wilkinson M J.Theuseofself-pollinated progeniesas ‘in-groups’ for the genetic characterization of cocoa germplasm[J]. Theoretical and applied genetics, 2000, 100: 160-166.
[11] Yeh F C, Yang R C, Boyle T B, et al.POPGENE, the user-friendly shareware for population genetic analysis [R]. Edmonton: Molecular biology and biotechnology centre, University of Alberta, 1997: 112-118.
[12] Liu K, Muse S V.PowerMarker an integrated analysis environment for genetic marker analysis[J]. Bioinformatics, 2005, 21: 2128-2129.
[13] Takezaki H, Nei M.Genetic distances and reconstruction of phylogenetic trees from microsatellite DNA[J]. Genetics, 1996, 144: 389-399.
[14] 段艳凤, 刘杰, 卞春松, 等. 中国88个马铃薯审定品种SSR指纹图谱构建与遗传多样性分析[J]. 作物学报, 2009, 35(8): 1451-1457.
[15] 罗忠霞, 房伯平, 李茹, 等. 基于EST-SSR标记的甘薯种质资源DNA指纹图谱构建[J]. 植物遗传资源学报, 2014, 15( 4): 810-814.
[16] 韩宗福, 王景会, 申贵芳, 等. 黄河流域棉花主要品种SSR指纹图谱构建及遗传差异分析[J]. 棉花学报, 2011, 23(6): 545-551.
[17] Ujihara T, Ohta R, Hayashi N, et al.Identification of Japanese and Chinese green tea cultivars by using simple sequence repeat markers to encourage proper labeling[J]. Bioscience, biotechnology, and biochemistry, 2009, 73(1): 15-20.
[18] 杨阳, 刘振, 赵洋, 等. 湖南省主要茶树品种分子指纹图谱的构建[J]. 茶叶科学, 2010, 30(5): 367-373.
[19] 刘本英, 孙雪梅, 李友勇, 等. 基于EST-SSR标记的云南无性系茶树良种遗传多样性分析及指纹图谱构建[J]. 茶叶科学, 2012, 32(3): 261-268.
[20] 王让剑, 杨军, 孔祥瑞, 等. 福建部分茶树品种SSR遗传差异分析与指纹图谱建立[C]//中国茶叶学会. 第十六届中国科协年会——分12茶学青年科学家论坛论文集. 昆明: 中国科学技术协会出版, 2014: 22.
[21] 刘峰, 冯雪梅, 钟文, 等. 适合棉花品种鉴定的SSR核心引物的筛选[J]. 分子植物育种, 2009, 6(7): 1160-1168.
[22] 赖运平, 张浙峰, 王丽容, 等. 利用SSR标记筛选DUS测试中甘蓝型油菜近似品种[J]. 分子植物育种, 2013, 11(2): 174-184.