The Role of Plasma Membrane H+-ATPase on Nitrogen-regulated Phosphorus Uptake in Tea Plants

CHEN Linmu; CHEN Jingguang; WANG Ningning; ZHANG Xianchen

doi:10.13305/j.cnki.jts.2019.06.011

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Journal of Tea Science ›› 2019, Vol. 39 ›› Issue (6) : 723-730. DOI: 10.13305/j.cnki.jts.2019.06.011

The Role of Plasma Membrane H⁺-ATPase on Nitrogen-regulated Phosphorus Uptake in Tea Plants

CHEN Linmu¹, CHEN Jingguang², WANG Ningning¹, ZHANG Xianchen^1,*

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Abstract

Phosphorus is one of the most important mineral elements for plant growth and development, and different nitrogen forms can regulate phosphorus uptake. As is well-known that plant plasma membrane (PM) H⁺-ATPase plays an important role in nutrition uptake, it may also relate to the different nitrogen-modulated phosphorus uptake. In our study, PM H⁺-ATPase in tea roots were isolated by two-phase partitioning in aqueous dextranT-500 and polyethylene glycol, and the effect of different nitrogen forms on phosphorus uptake and the characteristic parameters of PM H⁺-ATPase in tea roots were examined by using non-invasive micro-test technique and Western-blot. It was found that ammonium significantly improved the phosphorus accumulation in tea plants as compared with nitrate nutrition. In addition, less depolarized PM potentials, higher net H⁺ flux, PM H⁺-ATPase activities and protein levels were found under ammonium treatment than those under nitrate nutrition treatment. Furthermore, vanadate (the PM H⁺-ATPase inhibitor) significantly decreased phosphorus accumulation in tea plants under ammonium and nitrate nutrition treatments, which further suggests that PM H⁺-ATPase may be involved in phosphorus accumulation in tea plants regulated by different nitrogen forms.

Key words

ammoniacal nitrogen / nitrate nitrogen / phosphorus absorption / PM H⁺-ATPase / tea plants

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CHEN Linmu, CHEN Jingguang, WANG Ningning, ZHANG Xianchen. The Role of Plasma Membrane H⁺-ATPase on Nitrogen-regulated Phosphorus Uptake in Tea Plants[J]. Journal of Tea Science. 2019, 39(6): 723-730 https://doi.org/10.13305/j.cnki.jts.2019.06.011

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