Determination of Pesticide Residue in Fresh Tea Leaves and Dry Tea by Solid Extraction and Dispersive Solid Extraction Cleanup Coupled with Tandem Mass Spectrum

YANG Jie; ZHOU Li; YU Huan; SUN Hezhi; WANG Xinru; ZHANG Xinzhong; YANG Mei; CHEN Zongmao; LUO Fengjian

doi:10.13305/j.cnki.jts.2020.03.010

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Journal of Tea Science ›› 2020, Vol. 40 ›› Issue (3) : 397-406. DOI: 10.13305/j.cnki.jts.2020.03.010

Determination of Pesticide Residue in Fresh Tea Leaves and Dry Tea by Solid Extraction and Dispersive Solid Extraction Cleanup Coupled with Tandem Mass Spectrum

YANG Jie^1,2, ZHOU Li^1,*, YU Huan^1,2, SUN Hezhi¹, WANG Xinru¹, ZHANG Xinzhong¹, YANG Mei¹, CHEN Zongmao¹, LUO Fengjian^1,*

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Abstract

A method for the determination of 9 pesticide residues in fresh and dry tea was established. Residual pesticides in samples were extracted by acetonitrile, purified by Florisil/GCB solid phase extraction column, followed by absorbent of PSA and GCB as dispersed solid phase, and determined by ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) and gas chromatography-mass spectrometry (GC-MS). In the spiked range of 0.005-1.000 mg·kg^-1, the average recoveries of target pesticides in fresh leaves and dried tea were 70.3%-103.9%, and the relative standard deviations (RSD) were less than 20%. In the range of 0.005-2.000 mg·kg^-1, the linear relationship of target pesticides in fresh leaves and dry tea matrix was good, with r>0.995 4. The limit of quantity (LOQ) was 0.005 mg·kg^-1. The test results of actual samples showed that the method was highly sensitive and reproducible, and can meet the requirements of multi-residue detection.

Key words

dispersive solid-phase extraction / GC-MS / multi-residue of pesticides / solid phase extraction / tea / UPLC-MS/MS

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YANG Jie, ZHOU Li, YU Huan, SUN Hezhi, WANG Xinru, ZHANG Xinzhong, YANG Mei, CHEN Zongmao, LUO Fengjian. Determination of Pesticide Residue in Fresh Tea Leaves and Dry Tea by Solid Extraction and Dispersive Solid Extraction Cleanup Coupled with Tandem Mass Spectrum[J]. Journal of Tea Science. 2020, 40(3): 397-406 https://doi.org/10.13305/j.cnki.jts.2020.03.010

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