Vardenafil Improves the Proliferative Inhibition of EGCG-&#x003b2;-lactoglobulin Nanoparticles Against Liver Cancer Cells

CHEN Chunxiao; LOU Wenyu; DING Zhenjian; LI Zhuoye; YANG Yuanyuan; JIN Peng; DU Qizhen

doi:10.13305/j.cnki.jts.2020.04.009

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Journal of Tea Science ›› 2020, Vol. 40 ›› Issue (4) : 528-535. DOI: 10.13305/j.cnki.jts.2020.04.009

Research Paper

Vardenafil Improves the Proliferative Inhibition of EGCG-β-lactoglobulin Nanoparticles Against Liver Cancer Cells

CHEN Chunxiao, LOU Wenyu, DING Zhenjian, LI Zhuoye, YANG Yuanyuan, JIN Peng, DU Qizhen^*

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Abstract

The combination of nano and other drugs is an important strategy to improve the biological activity of EGCG, since a high EGCG concentration is essential for the inhibition of the proliferation of cancer cells. In this study, EGCG-vardenafil (VD)-β-lactoglobulin (β-Lg)-nanoparticles (EVβ-NPs) was prepared by encapsulating EGCG and VD in β-Lg nano-carriers. In vitro experimental results show that EVβ-NPs could upgrade the activity of caspase-3 in HepG2 cells compared to the native EGCG, which caused cell cycle arrest in the S phase of HepG2 cells to induce cell nuclear division, and finally lead to HepG2 cell apoptosis. The results demonstrate that the encapsulation of EGCG-VD can significantly improve the anticancer activity of EGCG, and possesses potential value in the development of EGCG anticancer products.

Key words

β-lactoglobulin / EGCG / nanoparticles / proliferative inhibition / vardenafil

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CHEN Chunxiao, LOU Wenyu, DING Zhenjian, LI Zhuoye, YANG Yuanyuan, JIN Peng, DU Qizhen. Vardenafil Improves the Proliferative Inhibition of EGCG-β-lactoglobulin Nanoparticles Against Liver Cancer Cells[J]. Journal of Tea Science. 2020, 40(4): 528-535 https://doi.org/10.13305/j.cnki.jts.2020.04.009

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