Cloning and Expression Analysis of CsPPH Gene in Tea Plant (Camellia sinensis)

ZHOU Zhe; CHEN Zhidan; WU Quanjin; XU Yilan; SUN Weijiang

doi:10.13305/j.cnki.jts.20200117.007

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Journal of Tea Science ›› 2020, Vol. 40 ›› Issue (1) : 39-50. DOI: 10.13305/j.cnki.jts.20200117.007

Cloning and Expression Analysis of CsPPH Gene in Tea Plant (Camellia sinensis)

ZHOU Zhe^1,2,4, CHEN Zhidan^2,3,4, WU Quanjin⁵, XU Yilan³, SUN Weijiang^1,2,3,4,*

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Abstract

Pheophytinase (PPH) is a key enzyme in chlorophyll degradation. It can convert pheophytin a into pheophorbide a, which is the last product to keep green in chlorophyll degradation pathway. This step is considered to be a key step of leaf senescence and yellowing. In this study, the full-length sequence of CsPPH gene was cloned from the new shoot leaves of albino tea plant Camellia sinensis cv. Baijiguan (MK359094), and its biological characteristics were analyzed. The full-length of CsPPH was 1 298 bp, and the ORF was 1 241 bp, encoding 413 amino acids. Sequence analysis indicated that the encoded protein was a stable hydrophobic protein, and its molecular weight was predicted to be 45 741.50 Da. Its theoretical isoelectric point was 6.12. It was mainly located in chloroplasts. The real-time fluorescence quantitative PCR showed that under shading conditions, the expression of CsPPH was inhibited, chlorophyll increased and leaf color turned green. Light promoted the expression of CsPPH in leaves of cv. Baijiguan, and chlorophyll degradation led to leaf albinism.

Key words

PPH gene / albinism of tea plant / bioinformatics analysis / clone

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ZHOU Zhe, CHEN Zhidan, WU Quanjin, XU Yilan, SUN Weijiang. Cloning and Expression Analysis of CsPPH Gene in Tea Plant (Camellia sinensis)[J]. Journal of Tea Science. 2020, 40(1): 39-50 https://doi.org/10.13305/j.cnki.jts.20200117.007

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