Effect of Three Antibiotic Treatments on Bacterial Endosymbiont Community of Ectropis Grisescens Warren

WANG Zhibo; BAI Jiahe; ZHOU Xiaogui; GUO Huawei; XIAO Qiang

doi:10.13305/j.cnki.jts.20201130.001

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Journal of Tea Science ›› 2021, Vol. 41 ›› Issue (1) : 90-100. DOI: 10.13305/j.cnki.jts.20201130.001

Research Paper

Effect of Three Antibiotic Treatments on Bacterial Endosymbiont Community of Ectropis Grisescens Warren

WANG Zhibo, BAI Jiahe, ZHOU Xiaogui, GUO Huawei, XIAO Qiang^*

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Abstract

In order to study the effects of three antibiotics on bacterial endosymbiont community of Ectropis grisescens, relative experiment was carried out with three antibiotics (tetracycline, cefalexin and rifampicin) at the concentration of 2.5 mg·mL^-1. Larvae of E. grisescens were fed the tea leaves with antibiotics for continuous 3 generations. Variation of endosymbiont community was studied using 16 S rDNA Illumina MiSeq technique. Besides, the Wolbachia of E. grisescens were detected basing on wsp gene. The results show that abundance and diversity of endosymbiont community in E. grisescens were increased and reduced, respectively under antibiotic treatments. The tetracycline and rifampicin could effectively remove Wolbachia from E. grisescens. Further, the population of E. grisescens without Wolbachia was established, which laid the foundation for the study on function of Wolbachia.

Key words

Ectropis grisescens / Wolbachia / antibiotics / symbiotic bacteria

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WANG Zhibo, BAI Jiahe, ZHOU Xiaogui, GUO Huawei, XIAO Qiang. Effect of Three Antibiotic Treatments on Bacterial Endosymbiont Community of Ectropis Grisescens Warren[J]. Journal of Tea Science. 2021, 41(1): 90-100 https://doi.org/10.13305/j.cnki.jts.20201130.001

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