Screening, Identification and Culture Conditions of a High-virulent Isolate of Beauveria against Myllocerinus Aurolineatus

WANG Dingfeng; LI Liangde; LI Huiling; ZHANG Hui; WU Guangyuan

doi:10.13305/j.cnki.jts.2021.01.009

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Journal of Tea Science ›› 2021, Vol. 41 ›› Issue (1) : 101-112. DOI: 10.13305/j.cnki.jts.2021.01.009

Screening, Identification and Culture Conditions of a High-virulent Isolate of Beauveria against Myllocerinus Aurolineatus

WANG Dingfeng, LI Liangde, LI Huiling, ZHANG Hui, WU Guangyuan^*

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Abstract

Myllocerinus aurolineatus (Coleoptera: Curculionidae) is one of the most important pests in tea plantation, and chemical control is the usual treatment method for this weevil at present. In order to explore alternative strategy for sustainable control of the weevil, dip method was employed to test the virulence of 12 isolates of Beauveria sp., which isolated from insects of Coleoptera. When inoculated with spore suspension (5.0×10⁷ spores·mL^-1), the strain Bbr1552 showed the highest virulence against the adults of M. aurolineatus, which caused the highest corrected mortality rates of 100%, shortest LT₅₀ of 4.49 d and highest cadaver rates of 69.44%. The LC₅₀ value was 1.55×10⁶ spores·mL^-1 on 7 d (25℃) after inoculation with Bbr1552. According to morphological identification and analysis of rDNA-ITS and Bloc sequences, the strain Bbr1552 was identified to be a B. brongniartii. Besides, the PPDA medium and 25℃ were the most optimal conditions for the colony growth and sporulation of the strain Bbr1552.

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Beauveria brongniartii / cultural condition / identification / Myllocerinus aurolineatus / virulence

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WANG Dingfeng, LI Liangde, LI Huiling, ZHANG Hui, WU Guangyuan. Screening, Identification and Culture Conditions of a High-virulent Isolate of Beauveria against Myllocerinus Aurolineatus[J]. Journal of Tea Science. 2021, 41(1): 101-112 https://doi.org/10.13305/j.cnki.jts.2021.01.009

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