茶叶儿茶素对发根农杆菌的抑制作用及抗酚菌株筛选研究

MAO Qing-li; SHI Zhao-peng; LI Lin; LIU Zhong-hua; ZHU Qi

doi:10.13305/j.cnki.jts.2007.03.006

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茶叶科学 ›› 2007, Vol. 27 ›› Issue (3) : 243-247. DOI: 10.13305/j.cnki.jts.2007.03.006

茶叶儿茶素对发根农杆菌的抑制作用及抗酚菌株筛选研究

毛清黎^1,2，施兆鹏²，李玲³，刘仲华²，朱旗²

作者信息 +

Study on Inhibitation of Agrobacterium rhizogenes by Tea Catechin and Screening of Anti-polyphenol Strain

MAO Qing-li^1,2, SHI Zhao-peng², LI Lin³, LIU Zhong-hua², ZHU Qi²

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文章历史 +

摘要

本文研究了茶叶儿茶素对发根农杆菌的抑制作用并进行了抗酚菌株的筛选。结果表明：儿茶素（TC80）对发根农杆菌有明显的抑制作用，其MIC90为25~100 μg/ml，这阐明了茶树中富含儿茶素等多酚类是制约农杆菌介导法对茶树进行遗传转化的关键因子。通过抗酚筛选获得的抗酚菌株R1000AP，其抗酚性提高10~40倍，其诱导茶树的发根频率提高46.5%（P<0.05）。

Abstract

This paper investigated inhibition of Agrobacterium rhizogenes by tea catechin and screening of the anti-polyphenol strain.The results showed that the catechin (TC80), which MIC90 was 25~100 μg/ml, showed the obvious inhibition of Agrobacterium rhizogenes. This showed that the plenty of polyphenols in the tea plant was a key factor to restrict Agrobacterium rhizogenes-mediated transformation of tea plant. An anti-polyphenol strain R1000AP obtained by anti-polyphenol screening, which anti-polyphenol capability enhanced 10~40 times. The hairy root frequency induced by the strain increased by 46.5%（P<0.05）compared to the non-anti-polyphenol strain.

导出引用

毛清黎, 施兆鹏, 李玲, 刘仲华, 朱旗. 茶叶儿茶素对发根农杆菌的抑制作用及抗酚菌株筛选研究[J]. 茶叶科学. 2007, 27(3): 243-247 https://doi.org/10.13305/j.cnki.jts.2007.03.006

MAO Qing-li, SHI Zhao-peng, LI Lin, LIU Zhong-hua, ZHU Qi. Study on Inhibitation of Agrobacterium rhizogenes by Tea Catechin and Screening of Anti-polyphenol Strain[J]. Journal of Tea Science. 2007, 27(3): 243-247 https://doi.org/10.13305/j.cnki.jts.2007.03.006

中图分类号： S571.1 Q939.11+4

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