茶树在干旱条件下的mRNA差异表达

陈盛相; 齐桂年; 夏建冰; 邹瑶; 单虹丽

doi:10.13305/j.cnki.jts.2012.01.012

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茶叶科学 ›› 2012, Vol. 32 ›› Issue (1) : 53-58. DOI: 10.13305/j.cnki.jts.2012.01.012

茶树在干旱条件下的mRNA差异表达

陈盛相, 齐桂年^*, 夏建冰, 邹瑶, 单虹丽

作者信息 +

mRNA Differential Expressionof Camellia sinensis under Drought Conditions

CHEN Sheng-xiang, QI Gui-nian^*, XIA Jian-bing, ZOU Yao, SHAN Hong-li

Author information +

文章历史 +

摘要

采用20%PEG-6000对福鼎大白茶无性系扦插苗进行模拟干旱处理,应用mRNA差异显示技术研究干旱与正常浇水对照的mRNA差异表达,发现1个在干旱条件下减量表达片段N_3-5。序列分析和同源性比对表明,N_3-5与茶树无性系Sajin茶叶色突变基因组序列标签S31.B15（Accession：DQ443473.1）有75%的同源性。根据序列相似同源基因的功能推测,该片段可能与茶树的抗旱机制有关。

Abstract

20% PEG-6000 was used for simulating drought treatment to Camellia sinensis cv. Fuding-dabaicha clone. mRNA differential display reverse transcriptase polymerase chain reaction (DDRT-PCR) was used to study the mRNA differential expression between drought stress and irrigated contro1. One down regulated gene was found under drought stress. Sequence analysis and homology alignment showed that N_3-5 showed 75% homogenicity with the Camellia sinensis clone Sajin tea leaf mutant color tag S31.B15 genomic sequence (Accession：DQ443473.1). It was suggested that the fragments N_3-5 was related to drought tolerance mechanism of tea plant according to the functions of their homologous sequences.

导出引用

陈盛相, 齐桂年, 夏建冰, 邹瑶, 单虹丽. 茶树在干旱条件下的mRNA差异表达[J]. 茶叶科学. 2012, 32(1): 53-58 https://doi.org/10.13305/j.cnki.jts.2012.01.012

CHEN Sheng-xiang, QI Gui-nian, XIA Jian-bing, ZOU Yao, SHAN Hong-li. mRNA Differential Expressionof Camellia sinensis under Drought Conditions[J]. Journal of Tea Science. 2012, 32(1): 53-58 https://doi.org/10.13305/j.cnki.jts.2012.01.012

中图分类号： S571.1 Q756

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