“紫娟”普洱茶茶褐素的膜分离及其理化性质的初步研究

doi:10.13305/j.cnki.jts.2012.03.003

茶叶科学 ›› 2012, Vol. 32 ›› Issue (3): 189-196.doi: 10.13305/j.cnki.jts.2012.03.003

• • 下一篇

“紫娟”普洱茶茶褐素的膜分离及其理化性质的初步研究

张钦, 董立星, 李改青, 龚加顺^*

云南农业大学食品科学技术学院,云南昆明 650201

收稿日期:2011-10-08 修回日期:2011-12-31 出版日期:2012-06-15 发布日期:2019-09-05
通讯作者: gong199@163.com
作者简介:张钦（1988— ）,女,重庆万州人,硕士生,从事食品生物技术及天然产物开发利用研究。
基金资助:
国家自然科学基金资助项目（No. 30960241; 30760152）

Study on Membrane Separation and Its Physicochemical Properties of Theabrownin in ‘Zijuan’ Pu-erh Tea

ZHANG Qin, DONG Li-xing, LI Gai-qing, GONG Jia-shun^*

College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China

Received:2011-10-08 Revised:2011-12-31 Online:2012-06-15 Published:2019-09-05

摘要/Abstract

摘要： 以“紫娟”普洱茶为原料,分离制备茶褐素,采用膜技术对茶褐素分级,得到含量较多的3500Da透出部分和100000Da未透出部分,并对两部分进行理化性质分析。结果表明：100000Da未透出部分总酸性基团、羧基、酚羟基、多酚类及多糖含量均高于3500Da透出部分;100000Da未透出部分的多糖由核糖、鼠李糖、甘露糖、葡萄糖和半乳糖组成;而3500Da透出部分未检出单糖。研究显示,3500Da透出样和100000Da未透出部分中均含有-OH、-COOH,推测“紫娟”普洱茶茶褐素属苯多酚类物质,并含有酚性色素、多糖、蛋白质、脂类及生物碱等。

关键词: “紫娟”普洱茶, 茶褐素, 膜分离, 理化性质, 居里点热裂解气质联用（CP-GC-MS）

Abstract: 3500Da decomposed dialysate and 100000Da undecomposed dialysate of the theabrownin in ‘zijuan’ pu-erh tea as the main content were obtained by using membrane technology and their physicochemical properties were studied. The result showed that the total acidic functional group, carboxyl group, phenolic hydroxyl group,tea polyphenols, polysaccharose content of 100000Da undecomposed dialysate were higher than those of 3500Da decomposed dialysate. The polysaccharide isolated from 100000Da undecomposed dialysate was composed of ribose, rhamnose, mannose, glucose and galactose,but these monose were not detected in 3500Da decomposed dialysate. It suggested that the 3500Da decomposed dialysate and 100000Da undecomposed dialysate contained the -OH and -COOH groups. It was suggested that the theabrownin of the ‘zijuan’ pu-erh tea belonged to benzene polyphenolic substance and contained of polyphenolic pigments, lipids, polysaccharide, protein and alkaloids.

Key words: ‘zijuan’, pu-erh tea, theabrownin, physicochemical properties, physicochemical properties, CP-GC-MS

中图分类号:

TS272.5⁺4

张钦, 董立星, 李改青, 龚加顺. “紫娟”普洱茶茶褐素的膜分离及其理化性质的初步研究[J]. 茶叶科学, 2012, 32(3): 189-196. doi: 10.13305/j.cnki.jts.2012.03.003.

ZHANG Qin, DONG Li-xing, LI Gai-qing, GONG Jia-shun. Study on Membrane Separation and Its Physicochemical Properties of Theabrownin in ‘Zijuan’ Pu-erh Tea[J]. Journal of Tea Science, 2012, 32(3): 189-196. doi: 10.13305/j.cnki.jts.2012.03.003.

参考文献

[1] 周红杰, 龚加顺, 王星银, 等. 云南普洱茶: 第一版[M]. 昆明: 云南科技出版社, 2004.
[2] Sano M, Takenaka Y, Kojima R, et al. Effects of pu-erh tea on lipid metabolism in rats[J]. Chem Pharm Bull, 1986, 34(1): 221-228.
[3] Duh PD, Yen GC, Yen WJ, et al. Effects of pu-erh tea on oxidative damage and nitric oxide scavenging[J]. Journal of Agricultural and Food Chemistry, 2004, 52: 8169-8176.
[4] Wang BS, Yu HM, Chang LW, et al. Protective effects of pu-erh tea on LDL oxidation and nitric oxide generation in macrophage cells[J]. LWT-Food Science and Technology, 2008, 41, 1122-113.
[5] Kuo KL, Weng MS, Chiang CT, et al. Comparative studies on the hypolipidemic and growth suppressive effects of oolong, black, pu-erh, and green tea leaves in rats[J]. J Agric Food Chem, 2005, 53(2): 480-489.
[6] Cao ZH, Gu DH, Lin QY, et al. Effect of pu-erh tea on body fat and lipid profiles in rats with diet-induced obesity[J]. Phytotherapy Research, 2011, 25(2): 234-238.
[7] Chiang Chun-Te, Weng Meng-Shih, Lin-Shiau, et al. Pu-erh tea supplementation suppresses fatty acid synthase expression in the rat liver through downregulating akt and jnk signalings as demonstrated in human hepatoma HepG2 cells[J]. Oncol Res, 2006, 16(3): 119-128.
[8] Lu CH, Hwang SL.Polyphenol contents of Pu-Erh teas and their abilities to inhibit cholesterol biosynthesis in Hep G2 cell line[J]. Food Chem, 2008, 111: 67-71.
[9] Hou Y, Shao WF, Xiao R, et al. Pu-erh tea aqueous extracts lower atherosclerotic risk factors in a rat Hyperlipidemia model[J]. Experimental Gerontology, 2009, 44: 434-439.
[10] Ohe T, Marutani K, Nakase S.Catechins are not major components responsible for anti-genotoxic effects of tea extracts against nitroarenes[J]. Mutation Research, 2001, 496: 75-81.
[11] 包云秀, 夏丽飞, 李友勇, 等. 茶树新品种‘紫娟’[J]. 园艺学报, 2008, 35(6): 93.
[12] 杨大鹏. 云南普洱茶茶褐素主要化学成分的分离及结构鉴定[D]. 昆明: 云南农业大学, 2009.
[13] 秦谊, 龚加顺, 张惠芬, 等. 普洱茶茶褐素提取工艺及理化性质的初步研究[J]. 林产化学与工业, 2009, 29(6): 95-98.
[14] 黄意欢. 茶学实验技术[M]. 北京: 中国农业出版社, 1997: 125-127.
[15] HOSOYA T, KAWAMOTO H, SAKA S.Oxime-trimethylsilyl method for analysis of wood pyrolysat[J]. J Appl Anal Pyrolysis, 2006, 77: 121-126.
[16] POUWELS A D, EIJKEL G B, BOON J J.Curie-point pyrolysis-capillary gas chromatography-high-resolution mass spectrometry of microcrystalline cellulose[J]. J Anal Appl Pyrolysis, 1989, 14: 237-280.
[17] RAMIAH M V.Thermogravimetric and differential thermal analysis of cellulose,hemicellulose,and lignin[J]. Journal of Polymer Science, 1970, 14: 1323-1337.
[18] ELDER T J, SOLTES E J.Pyrolysis of lignocellulosic material phenolic constituents of a wood pyrolytic oil[J]. Wood and Fibre Science, 1980, 12(4): 217-226.
[19] FAIX O, MEIER D, FORTMANN I.Thermal degradation products of wood:Gas chromatographic separation and mass spectrometric characterisation of monomeric lignin derived products[J]. Holz als Roh-und Werkstoff, 1990, 48: 281-285.
[20] FAIX O, MEIER D, FORTMANN I.Thermal degradation products of wood:A collection of electron-impact(EI)mass spectra of monomeric lignin derived products[J]. Holz als Roh-und Werkstoff, 1990, 48: 351-354.

“紫娟”普洱茶茶褐素的膜分离及其理化性质的初步研究

Study on Membrane Separation and Its Physicochemical Properties of Theabrownin in ‘Zijuan’ Pu-erh Tea

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 12

Metrics

本文评价

推荐阅读 10

[1]	龚受基, 滕翠琴, 梁东姨, 曹惠怡, 蒙彦妃, 张均伟, 谢加仕. 六堡茶茶褐素体外降脂功效研究[J]. 茶叶科学, 2020, 40(4): 536-543.
[2]	王峰, 陈玉真, 吴志丹, 江福英, 张文锦, 翁伯琦, 尤志明. 施用生物质炭对酸性茶园土壤氨挥发的影响[J]. 茶叶科学, 2017, 37(1): 60-70.
[3]	刘建, 彭春秀, 盛军, 高斌, 龚加顺. 普洱茶茶褐素对大鼠尿液影响的代谢组学研究[J]. 茶叶科学, 2016, 36(5): 491-499.
[4]	岳随娟, 刘建, 龚加顺. 普洱茶茶褐素对大鼠肠道菌群的影响[J]. 茶叶科学, 2016, 36(3): 261-267.
[5]	贺国文, 钟桐生, 彭晓赟, 龙立平, 赵运林, 章卫民. 茯砖茶褐素组分特征及对α-葡萄糖苷酶活性抑制研究[J]. 茶叶科学, 2016, 36(1): 102-110.
[6]	王秋萍, 龚加顺. “紫娟”普洱茶茶褐素对高脂饮食大鼠生长发育的影响[J]. 茶叶科学, 2012, 32(1): 87-94.
[7]	杨新河, 王丽丽, 黄建安, 吴文亮, 刘仲华. 普洱茶茶褐素的分级及相关性质初步研究[J]. 茶叶科学, 2011, 31(3): 187-194.
[8]	龚加顺, 陈一江, 彭春秀, 周红杰. 普洱茶发酵过程中不同添加物对茶褐素及其形成机制的影响[J]. 茶叶科学, 2010, 30(2): 101-108.
[9]	江和源, 陈小强, 寇小红, 王川丕, 崔宏春, 江用文. 茶多糖的分级纯化及组成分析[J]. 茶叶科学, 2007, 27(3): 248-252.
[10]	倪德江, 陈玉琼, 余志, 张芸, 谢笔钧, 周继荣. 乌龙茶加工过程多糖的变化、组分分离及特性研究[J]. 茶叶科学, 2005, 25(4): 282-288.
[11]	汪东风, 谢晓凤, 王世林, 郑俊, 严俊, 严鸿德, 王泽农. 茶多糖的组分及理化性质[J]. 茶叶科学, 1996, 16(01): 1-8.
[12]	王华夫, 陈瑞锋, 徐向群. 茶叶高活性天然抗氧化剂的制备工艺[J]. 茶叶科学, 1995, 15(01): 49-56.