做青工艺对乌龙茶香气组成化学模式的影响

doi:10.13305/j.cnki.jts.2014.04.011

摘要/Abstract

摘要： 在室内控温控湿环境[(22±1)℃、RH(71±4)%]条件下,借助茶叶香气成分GC-MS指纹图谱检测和代谢组学数据分析平台,以清香型乌龙茶、闽南乌龙茶和闽北乌龙茶工艺制法在制品和毛茶为对照样,探讨了做青工艺[萎凋程度（鲜叶减重率）×做青强度（摇青次数）]对做青叶在制品香气组成特征的影响。通过“云图”绘制和主成分分析结果表明,“晾青失水”可明显改变做青叶在制品香气组成的化学模式,伴随萎凋程度（鲜叶减重率）的加重,α-法呢烯、苯乙醛、香叶醇、3-己烯-1-醇和(Z)-己酸-3-己烯酯含量逐渐增多,而叶绿醇、吲哚、2,6-二叔丁基对甲酚和乙基苯等成分含量则呈减少趋势,但仅结合不同强度的“单次摇青”将无法促成乌龙茶特有香型品质。做青是形成乌龙茶特殊香型品质的关键工序,带有类似花果香型的吲哚、反式-橙花叔醇和苯乙醛等香气成分可作为乌龙茶做青工艺品质控制的主要化学评测指标。

关键词: 乌龙茶, 做青工艺, 香气成分, 模式识别

Abstract: Under controlled indoor air conditions set at (22±1)℃ with relative humidity of (71±4)%, a two-factor experiment, including degree of withering and intensity of rocking, was designed to investigate the effects of green-making technique on aroma characteristics of WIP (work in process) as compared with tea samples prepared during the respective process of Fresh-scent Oolong tea, South Fujian Oolong tea, and North Fujian Oolong tea. Aroma fingerprints of all tea samples were determined by gas chromatography coupled with mass spectrometry and processed with metabolomics analysis platform. The results from cloud plot and principal component analysis indicated that aroma pattern of WIPs would be obviously altered according to weight loss percentage of green leaves, accompanied with the increase on the contents of α-farnesene, benzeneacetaldehyde, geraniol, 3-hexen-1-ol and cis-3-hexenyl hexanoate, and the contents of phytol, indole, butylated hydroxytoluene and ethylbenzene were be decreased accordingly. However, the aromas pertinent to Oolong tea would not be fully formed only combinating on single shaking action with different intensity. Green-making was the essential procedure to bring about special aroma quality of Oolong tea, and the contents of floral scent compositions in tea samples, such as indole, trans-nerolidol, phenylacetaldehyde, etc., could be considered as the most important chemical indicators for quality control, in the green-making process of Oolong tea.

Key words: Oolong tea, green-making technology, aroma component, pattern recognition

中图分类号:

TS272.5⁺9

陈林, 陈键, 陈泉宾, 张应根, 宋振硕, 王丽丽, 尤志明. 做青工艺对乌龙茶香气组成化学模式的影响[J]. 茶叶科学, 2014, 34(4): 387-395. doi: 10.13305/j.cnki.jts.2014.04.011.

CHEN Lin, CHEN Jian, CHEN Quanbin, ZHANG Yinggen, SONG Zhenshuo, WANG Lili, YOU Zhiming. Effects of Green-making Technique on Aroma Pattern of Oolong Tea[J]. Journal of Tea Science, 2014, 34(4): 387-395. doi: 10.13305/j.cnki.jts.2014.04.011.

参考文献 36

[1]	陈林, 张方舟. 乌龙茶做青可控技术研究进展[J]. 福建茶叶, 2004(3): 16-18.
[2]	张天福, 林心炯, 焦海晏, 等. 乌龙茶做青工艺的研究[J]. 茶叶科学简报, 1989(3): 20-25.
[3]	张天福, 焦海晏, 林学诗, 等. 乌龙茶做青工艺与设备研究中间试验报告[J]. 茶叶科学简报, 1990(1): 1-4.
[4]	郭雅玲,杜连胜,林建民. 乌龙茶做青过程茶梢水势的变化[J]. 福建农业大学学报, 1994, 23(2): 148-153.
[5]	宿迷菊, 毛志方, 施海根, 等. 做青过程中水浸出物、茶多酚和氨基酸总量的变化研究[J]. 中国茶叶加工, 2007(3): 17-20.
[6]	黄福平, 陈荣冰, 梁月荣, 等. 乌龙茶做青过程中香气组成的动态变化及其与品质的关系[J]. 茶叶科学, 2003, 23(1): 31-37.
[7]	张秀云, 方世辉, 夏涛. 乌龙茶萎凋做青中β-葡萄糖苷酶活性变化研究[J]. 安徽农业大学学报, 2000, 27(2): 164-166.
[8]	唐颢, 杨伟丽, 文海涛. 乌龙茶新工艺做青期间果胶酶的活性变化及其生化效应研究[J]. 茶叶科学, 2005, 25(3): 197-202.
[9]	王登良, 魏新林, 张灵枝. 做青温度对岭头单枞乌龙茶香气成分影响的研究[J]. 茶叶科学, 2002, 22(1): 30-33.
[10]	张方舟, 陈荣冰, 李元钦, 等. 不同湿度做青环境对乌龙茶香气的影响[J]. 福建农业学报, 1999, 14(4): 34-37.
[11]	金心怡, 陈济斌, 王秀萍, 等. 做青环境调控方式对乌龙茶品质影响的试验研究[J]. 农业工程学报, 2003, 19(6): 215-218.
[12]	黄福平, 陈荣冰, 陈伟, 等. 做青强度对乌龙茶品质及其香气组成的影响[J]. 福建农业学报, 2001, 16(3): 28-34.
[13]	张方舟, 陈加友. 清香型乌龙茶空调做青设备及加工的关键技术[J]. 中国茶叶, 2005, 27(1): 32-33.
[14]	张方舟, 张应根, 陈林. 闽南乌龙茶的制法[J]. 中国茶叶, 2003, 26(2): 21-22.
[15]	陈林, 张方舟, 张应根. 闽北乌龙茶的制法[J]. 中国茶叶, 2003, 25(3): 17-18.
[16]	陈林, 张应根, 陈键, 等. 清香型乌龙茶品质形成过程中香气组成化学模式的动态变化规律[J]. 茶叶科学, 2013, 33(1): 53-59.
[17]	Tautenhahn R, Patti G J, Rinehart D, et al. XCMS Online: a web-based platform to process untargeted metabolomic data[J]. Anal Chem, 2012, 84(11): 5035-5039.
[18]	Patti G J, Tautenhahn R, Rinehart D, et al. A view from above: cloud plots to visualize global metabolomic data[J]. Anal Chem, 2013, 85(2): 798-804.
[19]	Barsch A, Lohmann W, Zurek G.The need for speed in metabolomics: UHPLC with maXis UHR-Q-TOF-MS analysis of tea extracts[J]. LC GC Eur, 2010, 16-17.
[20]	Ku K M, Choi J N, Kim J, et al. Metabolomics analysis reveals the compositional differences of shade grown tea (Camellia sinensis L.)[J]. J Agric Food Chem, 2010, 58(1): 418-426.
[21]	Lee J-E, Lee B-J, Hwang J-A, et al. Metabolic dependence of green tea on plucking positions revisited: a metabolomic study[J]. J Agric Food Chem, 2011, 59(19): 10579-10585.
[22]	Lee J-E, Lee B-J, Chung J-O, et al. Geographical and climatic dependencies of green tea (Camellia sinensis) metabolites: a 1H NMR-based metabolomics study[J]. J Agric Food Chem, 2010, 58(19): 10582-10589.
[23]	Fujimura Y, Kurihara K, Ida M, et al. Metabolomics-driven nutraceutical evaluation of diverse green tea cultivars[J]. PLoS One, 2011, 6(8): e23426-e23426.
[24]	Ku K M, Kim J, Park H-J, et al. Application of metabolomics in the analysis of manufacturing type of pu-erh tea and composition changes with different postfermentation year[J]. J Agric Food Chem, 2010, 58(1): 345-352.
[25]	Lee J-E, Lee B-J, Chung J-O, et al. 1H NMR-based metabolomic characterization during green tea (Camellia sinensis) fermentation[J]. Food Res Int, 2011, 44(2): 597-604.
[26]	郭丽, 谭俊峰, 王力, 等. 花香型绿茶加工工艺的研究[J]. 浙江农业科学, 2009(5): 946-948.
[27]	王小云, 杨春, 谭少波, 等. 乌龙花香型绿茶加工技术初探[J]. 广西农学报, 23(4): 47-48, 76.
[28]	潘玉华, 黄震标. 高香型坦洋工夫红茶初制工艺[J]. 福建茶叶, 2012(2): 15-18.
[29]	郭聪. 乌龙茶等时等转做青技术的探讨[J]. 茶叶科学简报, 1994(2): 27-28.
[30]	尤伟平. 乌龙茶摇青叶内源乙烯的测定[J]. 茶叶科学简报, 1989(1): 30-32.
[31]	尤伟平. 乌龙茶摇青叶内源乙烯生物合成途径与影响因子的探讨[J]. 茶叶科学简报, 1989(2): 27-31.
[32]	Tokitomo Y, Ikegami M, Yamanishi T, et al. Effects of withering and mass-rolling processes on the formation of aroma components in pouchong type semi-fermented tea[J]. Agric Biol Chem, 1984, 48(1): 87-91.
[33]	Kobayashi A, Tachiyama K, Kawakami M, et al. Effects of solar-withering and turn over treatment during indoor-withering on the formation of pouchong tea aroma[J]. Agric Biol Chem, 1985, 49(6): 1655-1660.
[34]	樊荣辉, 黄敏玲, 钟淮钦, 等. 花香的生物合成、调控及基因工程研究进展[J]. 中国细胞生物学学报, 2011, 33(9): 1028-1036.
[35]	刘乾刚, 林智, 蔡建明. 乌龙茶制造与品质形成的化学机理[J]. 福建农林大学学报: 自然科学版, 2002, 31(3): 346-351.
[36]	Chen Y L, Duan J, Jiang Y M, et al. Production, quality, and biological effects of oolong tea (Camellia sinensis)[J]. Food Rev Int, 2010, 27(1): 1-15.