茶树GAGP基因克隆及表达分析

doi:10.13305/j.cnki.jts.2015.01.012

茶叶科学 ›› 2015, Vol. 35 ›› Issue (1): 64-72.doi: 10.13305/j.cnki.jts.2015.01.012

茶树GAGP基因克隆及表达分析

李远华¹, 陆建良², 范方媛², 石玉涛¹

1. 武夷学院茶与食品学院/福建省武夷茶资源创新利用重点实验室/福建省高校茶叶工程研究中心/中国乌龙茶产业协同创新中心（培育）,福建武夷山 354300;
2. 浙江大学茶叶研究所,浙江杭州 310058

收稿日期:2014-09-09 修回日期:2014-10-13 出版日期:2015-02-15 发布日期:2019-08-23
作者简介:李远华,男,教授,博士,主要从事茶学及生物技术研究,E-mail: yhli@wuyiu.edu.cn
基金资助:
国家自然科学基金面上项目（31070613）、福建省科技厅重点项目（2012N0025）、国家级大学生创新创业训练计划项目（201310397001）; 国家自然科学基金面上项目（31070613）、福建省科技厅重点项目（2012N0025）、国家级大学生创新创业训练计划项目（201310397001）

Gene Cloning and Expression Analysis of GAGP in Tea Plant

LI Yuanhua¹, LU Jianliang², FAN Fangyuan², SHI Yutao¹

1. College of Tea and Food Science, Wuyi University, Wuyishan 354300, China;
2. Tea Research Institute of Zhejiang University, Hangzhou 310058, China

Received:2014-09-09 Revised:2014-10-13 Online:2015-02-15 Published:2019-08-23

摘要/Abstract

摘要： 采用SSH技术分析了VA菌根处理后福鼎大白茶根系基因差异表达情况,获得了差异序列,序列比对显示,在下调表达序列中可能包含了10种未知功能的基因;在上调表达序列中可能包含了5种可能的基因。采用RACE技术获得GAGP基因长3β146βbp（GenBank,登录号KJ946251）,具有2β769βbp开放阅读框（1^st~2β769^th）,编码923个氨基酸。分子生物信息学分析表明,GAGP蛋白分子量约106.9βkD,等电点为8.42,定位于线粒体内。定量PCR分析表明,GAGP在茶树叶片中表达强度存在明显的品种差异,GAGP对生物性和非生物性胁迫均有明显响应。

关键词: 茶树, GAGP, 基因克隆, 表达

Abstract: By using SSH, the differences in gene expression of root from Fudingdabai tea plant infected by VA mycorrhiza were analyzed and the diversity sequences was obtained. The sequence alighment showed that the down-regulated expression sequence possibly contained 10 unknown genes and the up-regulated expression sequence possibly contained 5 known genes. The GAGP (gap-pol) genic full-length sequence was obtained by using RACE. The length of GAGP gene was 3146bp (GenBank, Accession no., KJ946251), with 2β769βbp ORF (1^st-2β769^th), the sequence encoded 923 amino acid. Bioinformatics indicated that the GAGP protein’s molecular weight was about 106.9βkD, IEP was 8.42, located in mitochondria. The study also showed expression degree of GAGP was distinct in different cultivars, while it responded obviously to biological and non-biological stress.

Key words: tea plant, GAGP, gene clone, expression

中图分类号:

S571.1
Q52

李远华, 陆建良, 范方媛, 石玉涛. 茶树GAGP基因克隆及表达分析[J]. 茶叶科学, 2015, 35(1): 64-72. doi: 10.13305/j.cnki.jts.2015.01.012.

LI Yuanhua, LU Jianliang, FAN Fangyuan, SHI Yutao. Gene Cloning and Expression Analysis of GAGP in Tea Plant[J]. Journal of Tea Science, 2015, 35(1): 64-72. doi: 10.13305/j.cnki.jts.2015.01.012.

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茶树GAGP基因克隆及表达分析

Gene Cloning and Expression Analysis of GAGP in Tea Plant

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