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茶叶科学 ›› 2015, Vol. 35 ›› Issue (1): 97-102.doi: 10.13305/j.cnki.jts.2015.01.018

• • 上一篇    

抗茶树冰核细菌内生菌的筛选及鉴定

黄晓琴1,2, 张丽霞1,2, 刘会香3, 陈宗懋4, 李多川3,*   

  1. 1. 山东农业大学园艺科学与工程学院,山东 泰安 271018;
    2. 作物生物学国家重点实验室,山东 泰安 271018;
    3. 山东农业大学植保学院,山东 泰安 271018;
    4. 中国农业科学院茶叶研究所,浙江 杭州 310008
  • 收稿日期:2014-08-12 修回日期:2014-12-15 出版日期:2015-02-15 发布日期:2019-08-23
  • 通讯作者: *
  • 作者简介:黄晓琴,女,副教授,主要从事茶树生理生态和茶文化、茶叶经济方面的研究。
  • 基金资助:
    山东省自然基金项目(ZR2011CQ009)

Screeining and Identification of the Endophytic Bacterial Strain Against Ice Nucleation Active Bacteria of Tea Plant

HUANG Xiaoqin1,2, ZHANG Lixia1,2, LIU Huixiang3, CHEN Zongmao4, LI Duochuan3, *   

  1. 1. College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an 271018, China;
    2. Shandong Key Laboratory of Crop Biology, Tai’an 271018, China;
    3. Department of Plant Pathology, Shandong Agricultural University, Tai’an 271018, China;
    4. Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China
  • Received:2014-08-12 Revised:2014-12-15 Online:2015-02-15 Published:2019-08-23

摘要: 从茶树内生菌中进行了冰核细菌拮抗菌的筛选,得到菌株Y1,通过对菌株Y1进行形态学观察、生理生化指标测定及16βS rDNA序列测定和序列同源性分析,将菌株Y1鉴定为解淀粉芽孢杆菌(Bacillus amyloliquefaciens)。本研究获得了茶树内生拮抗菌株,明确了菌株Y1的种属,有利于冰核细菌生物防治的开展。

关键词: 茶树, 冰核细菌, 拮抗菌, 内生菌, 筛选, 鉴定

Abstract: In order to protect the tea plant against freezing injury, the screening of antagonistic bacteria against INA bacteria No. 8 (Pantoea ananatis) and No. 14 (P. agglomerans) isolated from tea plant was carried out in Shandong Province. The strain Y1,an antagonistic bacteria against INA bacteria was isolated from the endophytic bacteria strains of tea plant. Based on the morphological characteristics and 16βS rDNA sequence analysis, the strain Y1 was identified as the Bacillus amyloliquefaciens. Through this study, the endophytic bacteria strain against the ice nucleation bacteria was obtained and identefied, it will be beneficial to the development of the biological control of ice nucleation bacterial.

Key words: tea plant, ice nucleation active bacteria, antagonistic bacterial strain, endophytic strains, screening, identification

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