基于高通量测序的贮藏白牡丹表面细菌多样性分析

陈佳佳; 胡雲飞; 沈诗钰; 王志华; 周喆; 唐琴; 孙威江

doi:10.13305/j.cnki.jts.2020.04.008

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茶叶科学 ›› 2020, Vol. 40 ›› Issue (4) : 519-527. DOI: 10.13305/j.cnki.jts.2020.04.008

研究报告

基于高通量测序的贮藏白牡丹表面细菌多样性分析

陈佳佳^1,2,4, 胡雲飞^2,3,4, 沈诗钰^1,2,4, 王志华^1,2,4, 周喆^1,2,4, 唐琴^1,2,4, 孙威江^1,2,3,4,*

作者信息 +

Analysis of Bacterial Diversity of Stored White Peony by High-Throughput Sequencing

CHEN Jiajia^1,2,4, HU Yunfei^2,3,4, SHEN Shiyu^1,2,4, WANG Zhihua^1,2,4, ZHOU Zhe^1,2,4, TANG Qin^1,2,4, SUN Weijiang^1,2,3,4,*

Author information +

文章历史 +

摘要

为分析贮藏白牡丹细菌多样性,利用高通量测序对11个不同贮藏年份白牡丹细菌进行16 S rDNA检测。结果表明,贮藏茶样的优势菌门为变形菌门（Proteobacteria）、拟杆菌门（Bacteroidetes）、厚壁菌门（Firmicutes）等,优势菌纲为α-变形菌纲（Alphaproteobacteria）、γ-变形菌纲（Gammaproteobacteria）、鞘脂杆菌纲（Sphingobacteriia）等,优势菌属为鞘氨醇单胞菌属（Sphingomonas）、甲基杆菌属（Methylobacterium）、土地杆菌属（Pedobacter）等。不同贮藏年份白牡丹细菌组成结构相似,丰度存在差异;贮藏年份对白牡丹表面细菌组成未有显著影响,而贮藏环境影响细菌多样性。

Abstract

To investigate the diversity of the bacterial communities in stored white peony, 16 S rDNA of bacteria collected from 11 samples of different storage durations were analyzed by high-throughput sequencing. The results show that the species richness and diversity of DB13 were the highest. While in all stored white peony, the dominant phylum were Proteobacteria, Bacteroidetes and Firmicutes. The dominant class were Alphaproteobacteria, Gamma proteobacteria and Sphingobacteriia. And the dominant bacterial species at genus level include: Sphingomonas, Methylobacterium and Pedobacter. The composition structures of samples were similar, but there was difference in their abundance. Storage duration had no significant effect on the bacteria composition of white peony, but environments played important roles.

导出引用

陈佳佳, 胡雲飞, 沈诗钰, 王志华, 周喆, 唐琴, 孙威江. 基于高通量测序的贮藏白牡丹表面细菌多样性分析[J]. 茶叶科学. 2020, 40(4): 519-527 https://doi.org/10.13305/j.cnki.jts.2020.04.008

CHEN Jiajia, HU Yunfei, SHEN Shiyu, WANG Zhihua, ZHOU Zhe, TANG Qin, SUN Weijiang. Analysis of Bacterial Diversity of Stored White Peony by High-Throughput Sequencing[J]. Journal of Tea Science. 2020, 40(4): 519-527 https://doi.org/10.13305/j.cnki.jts.2020.04.008

中图分类号： S571.1

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