茶树CsbHLH024和CsbHLH133转录因子功能鉴定

刘任坚; 王玉源; 刘少群; 舒灿伟; 孙彬妹; 郑鹏

doi:10.13305/j.cnki.jts.2022.03.001

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茶叶科学 ›› 2022, Vol. 42 ›› Issue (3) : 347-357. DOI: 10.13305/j.cnki.jts.2022.03.001

研究报告

茶树CsbHLH024和CsbHLH133转录因子功能鉴定

刘任坚¹, 王玉源¹, 刘少群¹, 舒灿伟², 孙彬妹¹, 郑鹏^1,*

作者信息 +

Functional Identification of CsbHLH024 and CsbHLH133 Transcription Factors in Tea Plants

LIU Renjian¹, WANG Yuyuan¹, LIU Shaoqun¹, SHU Canwei², SUN Binmei¹, ZHENG Peng^1,*

Author information +

文章历史 +

摘要

茶树叶片毛状体含有多种次生代谢产物,在茶叶外观质量以及茶树响应生物和非生物胁迫方面起着重要作用。通过双荧光分子互补（BiFC）试验、GUS活性染色试验以及过表达试验对茶树叶片毛状体相关候选基因CsbHLH024和CsbHLH133的功能进行鉴定。结果表明,CsbHLH024/CsbHLH133和CsTTG1蛋白在植物中能够相互作用,并且它们的启动子能够在叶片组织中驱动下游基因的表达。进一步将它们分别过表达到野生型拟南芥Col和对应的拟南芥纯合突变体中,发现它们能够影响拟南芥叶片毛状体的形成,恢复突变体的表型,并引起毛状体相关基因表达水平的变化。本研究为进一步揭示茶树叶片毛状体形成的分子调控机制提供理论依据。

Abstract

Tea plant leaf trichomes contain various secondary metabolites and play an important role in the tea appearance quality as well as the response of tea plants to biotic and abiotic stresses. In this study, the function of leaf trichome-related genes CsbHLH024 and CsbHLH133 were analyzed using Bimolecular Fluorescent Complimentary (BiFC), GUS staining and overexpression experiments. The results show that CsbHLH024/CsbHLH133 and CsTTG1 could interact in plants, and their promoters could drive downstream gene expression in leaf tissues. They were further transformed into wild Arabidopsis thaliana (Col) and corresponding homozygous mutants, respectively to get overexpression lines. Both genes could affect the leaf trichome formation in Arabidopsis thaliana, restore the phenotype of the mutants, and induce the expression levels of trichome-related genes. This study provided a theoretical basis for further research on the molecular regulation mechanism of trichome formation in tea leaves.

导出引用

刘任坚, 王玉源, 刘少群, 舒灿伟, 孙彬妹, 郑鹏. 茶树CsbHLH024和CsbHLH133转录因子功能鉴定[J]. 茶叶科学. 2022, 42(3): 347-357 https://doi.org/10.13305/j.cnki.jts.2022.03.001

LIU Renjian, WANG Yuyuan, LIU Shaoqun, SHU Canwei, SUN Binmei, ZHENG Peng. Functional Identification of CsbHLH024 and CsbHLH133 Transcription Factors in Tea Plants[J]. Journal of Tea Science. 2022, 42(3): 347-357 https://doi.org/10.13305/j.cnki.jts.2022.03.001

中图分类号： S571.1

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