茶树PATL基因家族鉴定及CsPATL1上游转录调控分析

王金波; 谢思艺; 窦祥亚; 申小华; 田娜; 刘硕谦

doi:10.13305/j.cnki.jts.2025.02.005

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茶叶科学 ›› 2025, Vol. 45 ›› Issue (2) : 191-200. DOI: 10.13305/j.cnki.jts.2025.02.005

研究报告

茶树PATL基因家族鉴定及CsPATL1上游转录调控分析

王金波¹, 谢思艺¹, 窦祥亚¹, 申小华¹, 田娜^1,2, 刘硕谦^1,2,*

作者信息 +

Identification of CsPATL Gene Family and Analysis of Upstream Transcriptional Regulation of CsPATL1

WANG Jinbo¹, XIE Siyi¹, DOU Xiangya¹, SHEN Xiaohua¹, TIAN Na^1,2, LIU Shuoqian^1,2,*

Author information +

文章历史 +

摘要

Patellin（PATL）基因家族对植物生长发育和环境适应性有着重要影响。对茶树PATL（CsPATL）基因家族进行了系统鉴定和详细分析,通过多种生物信息学方法,成功鉴定出CsPATL家族的5个成员,并对其蛋白序列的理化性质进行了分析,结果表明,CsPATL家族的5个成员编码氨基酸232~585个,蛋白质分子质量为26.31~64.69 kDa,等电点为4.65~9.35。同时,对这些基因的染色体定位及其启动子序列中的顺式元件进行了详细分析,发现这些基因不均匀地分布在4条染色体上,主要参与植物激素响应、非生物胁迫响应和光响应。通过酵母单杂交试验、凝胶电泳迁移试验和双荧光素酶试验验证了Cshdz7可以直接结合CsPATL1的启动子并促进CsPATL1的表达。这些发现为理解CsPATL家族基因在茶树发育调控中的作用提供了新的视角。

Abstract

The Patellin (PATL) gene family is essential for plant growth, development and environmental adaptation. This study systematically identified and analyzed the CsPATL gene family in tea plants (Camellia sinensis). Five members of the CsPATL family were identified using a variety of bioinformatics techniques, and the physicochemical characteristics of their protein sequences were analyzed. The results show that the five CsPATL genes encode 232~585 amino acids, their molecular weights are 26.31~64.69 kDa, and their theoretical isoelectric points are 4.65~9.35. The chromosomal localization of these genes and the cis-elements in their promoter sequences were also analyzed in detail, and it was found that these genes were unevenly distributed on four chromosomes, and were mainly involved in phytohormone response, abiotic stress response, and light response, with light-responsive elements occupying a significant proportion in particular. Y1H, EMSA, and dual-luciferase assays confirm that Cshdz7 can directly bind to the CsPATL1 promoter and promote CsPATL1 gene expression. These findings provided a new perspective on the role of the CsPATL family genes in plant developmental regulation of tea plant development.

导出引用

王金波, 谢思艺, 窦祥亚, 申小华, 田娜, 刘硕谦. 茶树PATL基因家族鉴定及CsPATL1上游转录调控分析[J]. 茶叶科学. 2025, 45(2): 191-200 https://doi.org/10.13305/j.cnki.jts.2025.02.005

WANG Jinbo, XIE Siyi, DOU Xiangya, SHEN Xiaohua, TIAN Na, LIU Shuoqian. Identification of CsPATL Gene Family and Analysis of Upstream Transcriptional Regulation of CsPATL1[J]. Journal of Tea Science. 2025, 45(2): 191-200 https://doi.org/10.13305/j.cnki.jts.2025.02.005

中图分类号： S571.1 Q52

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