非水溶性茶叶蛋白降血脂作用的研究

活泼; 黄光荣; 张晓晖; 夏泳; 蒋家新; 傅剑云; 章克昌

doi:10.13305/j.cnki.jts.2005.02.003

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茶叶科学 ›› 2005, Vol. 25 ›› Issue (2) : 95-99. DOI: 10.13305/j.cnki.jts.2005.02.003

非水溶性茶叶蛋白降血脂作用的研究

活泼¹, 黄光荣¹, 张晓晖³, 夏泳², 蒋家新¹, 傅剑云², 章克昌³

作者信息 +

Studies on Lipid-depressing Function of Water Insoluble Tea Protein

HUO Po¹, HUANG Guang-rong¹, ZHANG Xiao- hui³, XIA Yong², JIANG Jia-xin¹, FU Jian-yun², ZHANG Ke-chang³

Author information +

文章历史 +

摘要

研究了采用碱法从茶叶中提取非水溶性茶叶蛋白的工艺,茶叶蛋白提取率达61.1%。通过调节提取液pH值的方法制备粗蛋白,粗蛋白纯度为54.45%;以高血脂症大鼠为模型,研究了非水溶性茶叶蛋白的降血脂作用。结果表明：非水溶性茶叶蛋白能明显降低高血脂症大鼠血清TC、TG、LDL-C、AI和R-CHR指标（P<0.05）,对HDL-C有一定的调节作用;证明非水溶性茶叶蛋白有明显的降血脂效果,对抗动脉粥样硬化及冠心病可能有一定的预防作用。

Abstract

Alkali extraction technology of water insoluble protein from tea was studied. The extracting rate of tea protein was around 61.1%. The crude tea protein was prepared by adjusting pH of the extract and its purity was 54.45%.The effects of water insoluble tea protein on decreasing blood lipids were studied on the hyperlipidemia rats. Results showed that the protein had significant effects on decreasing TC、TG、LDL-C、AI and R-CHR in the hyperlipidemia rats(p<0.05). It could also modulate the HDL-C indexes. The results confirmed that the water insoluble tea protein had remarkable effects on decreasing the blood lipid of hyperlipidemia and possibly is preventing atherosclerosis and coronary heart disease.

导出引用

活泼, 黄光荣, 张晓晖, 夏泳, 蒋家新, 傅剑云, 章克昌. 非水溶性茶叶蛋白降血脂作用的研究[J]. 茶叶科学. 2005, 25(2): 95-99 https://doi.org/10.13305/j.cnki.jts.2005.02.003

HUO Po, HUANG Guang-rong, ZHANG Xiao- hui, XIA Yong, JIANG Jia-xin, FU Jian-yun, ZHANG Ke-chang. Studies on Lipid-depressing Function of Water Insoluble Tea Protein[J]. Journal of Tea Science. 2005, 25(2): 95-99 https://doi.org/10.13305/j.cnki.jts.2005.02.003

中图分类号： TS272 R972+.5

参考文献

[1] 安徽农学院. 茶叶生物化学, 第二版[M]. 北京, 农业出版社, 1988: 3~5.
[2] 汪东风, 谢晓凤, 严俊等. 茶多糖等有效成分综合提取研究[J]. 中草药, 1998, 29(11): 739~740.
[3] 李燕, 蔡东联, 等. 茶叶蛋白液预防辐射引起的突变效应[J]. 癌变·畸变·突变, 2001, 13(1): 32~35.
[4] 汪家政, 范明. 蛋白质技术手册[M]. 科学出版社, 2002: 65~67.
[5] 北京大学生物化学教研室. 生物化学实验指导[M]. 北京: 人民教育出版社, 1979.
[6] DOMINGUEZ H,NUNEZ M J.Enzymatic Pretreatment to Enhance Oil Extraction from Fruits and Oilseeds[J]. A Review Food Chemistry. 1994, 49: 291~286.
[7] ROBERT D HAGENMAIER.Aqueous Processing of Full-Fai Sunflower Seed[J]. Yields of Oil and Protein.1974: 470~471.
[8] 杨玺. 血脂异常及高粘度症防治必读[M]. 上海科学技术文献出版社, 2002: 18~30.
[9] 白堃元. 茶叶加工[M]. 北京,化学工业出版社, 2001: 1~6.
[10] 姚慧英, 刘启贵. 茶叶深加工的意义和作用[J]. 茶报, 2001(2): 30~32.