白叶1号的低温抗逆特性分析

摘要/Abstract

摘要： 比较了浙江省白化品种白叶1号返白阶段和主栽品种龙井43的低温抗性。结果表明,常温（25℃）环境下,返白期白叶1号反映光合能力的净光合速率Pn和最大羧化反应效率Vc_max都只有龙井43的70%~80%,而APX、POD等抗氧化酶活力比龙井43高30%以上。低温（2℃）处理24βh后,白叶1号反映光合能力的各项参数反而高于龙井43,同时其APX、POD、SOD活性也比龙井43分别高38.9%、33.3%、23.3%。说明白叶1号可能由于白化现象的产生,导致其抗氧化系统修复能力得到提高,因此在低温胁迫中,能够更为有效地清除叶片H₂O₂累积,缓解H₂O₂造成的氧化胁迫及光合抑制现象,具备比龙井43更好的低温耐受性。

关键词: 白叶1号, 返白, 低温胁迫, 抗氧化酶

Abstract: The cold resistance was compared in two cultivars, the albino Baiye 1 (Camellia sinensis cv. Baiye 1) during albinism period and Longjing 43 (Camellia sinensis cv. Longjing 43) in Zhejiang province. The results showed that the net photosynthesis rate (Pn) and maximum carboxylation rate of Rubisco (Vc_max) in Baiye 1 during the albinism period were only 70%-80% of those in Longjing 43, but the activities of antioxidant enzymes APX and POD were both 30% higher than those in Longjing 43 under normal temperature. However, when they were treated under 2℃ condition for 24βh, various parameters related to photosynthesis were higher in Baiye 1 than Longjing 43. The activities of APX, POD and SOD were also 38.9%, 33.3% and 23.3% higher in Baiye 1. All the results indicated that the albinism phenomenon of Baiye 1 may lead to the improvement of antioxidant system, which would effectively reduce the H₂O₂accumulation in tea leaves and alleviate oxidative damage and photosynthetic inhibition during cold stress. Therefore, Baiye 1 during albinism period may have a better cold resistance than Longjing 43.

Key words: Baiye 1, albinism, cold stress, antioxidant enzymes

中图分类号:

S571.1
P423

张兰, 李鑫, 魏吉鹏, 李治鑫, 沈晨, 颜鹏, 张丽平, 韩文炎. 白叶1号的低温抗逆特性分析[J]. 茶叶科学, 2017, 37(1): 71-77.

ZHANG Lan, LI Xin, WEI Jipeng, LI Zhixin, SHEN Chen, YAN Peng, ZHANG Liping, HAN Wenyan. Analysis of the Cold Resistance of Baiye 1[J]. Journal of Tea Science, 2017, 37(1): 71-77.

参考文献 31

[1]	成浩, 李素芳, 陈明, 等. 白叶1号特异性状的生理生化本质[J]. 茶叶科学, 1999, 19(2): 87-92.
[2]	李素芳, 陈树尧, 成浩, 等. 茶树阶段性返白现象的初步研究[J]. 中国茶叶, 1994, 16(2): 26-27.
[3]	曾超珍, 刘仲华. 白叶1号阶段性白化机理的研究进展[J]. 分子植物育种, 2015, 13(12): 2905-2911.
[4]	陆文渊, 钱文春, 赖建红, 等. 白叶1号品质的气候成因初探[J]. 茶叶科学技术, 2012(3): 37-39.
[5]	何洁, 刘鸿先, 王以柔, 等. 低温与植物的光合作用[J]. 植物生理学报, 1986(2): 1-6.
[6]	李庆会, 徐辉, 周琳, 等. 低温胁迫对2个茶树品种叶片叶绿素荧光特性的影响[J]. 植物资源与环境学报, 2015, 24(2): 26-31.
[7]	杨亚军. 中国茶树栽培学[M]. 上海: 上海科学技术出版社, 2005: 37.
[8]	刘明炎, 王友平. 倒春寒对茶树的影响及预防[J]. 茶业通报, 2003, 25(1): 23-24.
[9]	聂雄平, 聂春平, 李鹰, 等. “倒春寒”对茶树的危害及预防补救措施[J]. 吉林农业, 2012(11): 112.
[10]	Caemmerer S V, Farquhar G D.Some relationships between the biochemistry of photosynthesis and the gas exchange of leaves[J]. Planta, 1981, 153(1): 376-387.
[11]	Ethier G J, Livingston N J.On the need to incorporate sensitivity to CO2 transfer conductance into the Farquhar-von Caemmerer-Berry leaf photosynthesis model[J]. Plant Cell & Environment, 2004, 27(2): 137-153.
[12]	Snel J F H, Kooten O V. The use of chlorophyll fluorescence and other noninvasive spectroscopic techniques in plant stress physiology[J]. Photosynth Res, 1990(25): 146-332.
[13]	Nakano Y, Asada K.Purification of ascorbate peroxidase in spinach chloroplasts; its inactivation in ascorbate-depleted medium and reactivation by monodehydroascorbate radical[J]. Plant and Cell Physiology, 1987, 28(1): 131-140.
[14]	Cakmak I, Marschner H.Magnesium deficiency and high light intensity enhance activities of superoxide dismutase, ascorbate peroxidase and glutathione reductase in bean leaves[J]. Plant Physiology, 1992, 98(4): 1222-1227.
[15]	Giannopolitis C N, Ries S K.Superoxide dismutases: II. purification and quantitative relationship with water-soluble protein in seedlings[J]. Plant Physiology, 1977, 59(2): 315-318.
[16]	Farquhar G D, Sharkey T D.Stomatal conductance and photosynthesis[J]. Annual Reviews of Plant Physiology, 2003, 33(33): 317-345.
[17]	Baker NR.Chlorophyll fluorescence: a probe of photosynthesis in vivo[J]. Annual Review of Plant Biology, 2008, 59(59): 89-113.
[18]	Farquhar G D, Caemmerer S V, Berry JA.A biochemical model of photosynthetic CO2 assimilation in leaves of C3 species[J]. Planta, 1980, 149(1): 78-90.
[19]	Tcherkez G.The mechanism of Rubisco-catalyzed oxygenation[J]. Plant Cell & Environment, 2015, 13(1): 28-40.
[20]	Torres M A, Dangl J L, Jones J D.Arabidopsis gp91phox homologues AtrbohD and AtrbohF are required for accumulation of reactive oxygen intermediates in the plant defense response[J]. Proceedings of the National Academy of Sciences, 2002, 99(1): 517-522.
[21]	Alscher R G, Donahue J L, Cramer C L.Reactive oxygen species and antioxidants: Relationships in green cells[J]. Physiologia Plantarum, 1997, 100(2): 224-233.
[22]	朱明库, 胡宗利, 周爽, 等. 植物叶色白化研究进展[J]. 生命科学, 2012(3): 255-261.
[23]	Li Q, Huang J A, Liu S Q, et al.Proteomic analysis of young leaves at three developmental stages in an albino tea cultivar[J]. Proteome Science, 2011, 9(1): 44.
[24]	成浩, 陈明, 虞富莲, 等. 茶叶片阶段性返白过程中色素蛋白复合体的变化[J]. 植物生理学报, 2000, 36(4): 300-304.
[25]	李素芳, 陈明. 茶树阶段性返白现象的研究——RuBp羧化酶与蛋白酶的变化[J]. 中国农业科学, 1999, 32(3): 33-38.
[26]	Deng B L.Antioxidative response of Golden Agave leaves with different degrees of variegation under high light exposure[J]. Acta Physiologiae Plantarum, 2012, 34(5): 1925-1933.
[27]	Kim J S, Yun B W, Choi J S, et al.Death mechanisms caused by carotenoid biosynthesis inhibitors in green and in undeveloped plant tissues[J]. Pesticide Biochemistry & Physiology, 2002, 78(3): 127-139.
[28]	李璇, 岳红, 黄璐琦, 等. 环境胁迫下植物抗氧化酶的反应规律研究[C] //中国药学会. 中国药学大会暨第十四届中国药师周论文集. 2010: 527-534.
[29]	姜籽竹, 朱恒光, 张倩, 等. 低温胁迫下植物光合作用的研究进展[J]. 作物杂志, 2015(3): 23-28.
[30]	夏晓剑. 油菜素内酯调控黄瓜光合作用、抗逆性及农药代谢的生理与分子机理研究[D]. 杭州: 浙江大学蔬菜研究所, 2009: 70.
[31]	Tkachuk V A, Tyurin-Kuzmin P A, Belousov V V, et al. Hydrogen peroxide as a new second messenger[J]. Biochemistry Supplement, 2012, 29(1/2): 21-37.