雌蕊缺失茶树花3个发育期的数字基因表达谱分析

茶叶科学 ›› 2017, Vol. 37 ›› Issue (1): 97-107.

雌蕊缺失茶树花3个发育期的数字基因表达谱分析

李梅¹, 陈林波¹, 田易萍¹, 夏丽飞¹, 宋维希¹, 梁名志¹, 江昌俊^2,*

1. 云南省农业科学院茶叶研究所云南省茶树种质资源创新与配套栽培技术工程研究中心,云南勐海 666201;
2. 安徽农业大学茶树生物学与资源利用国家重点实验室,安徽合肥 230036

收稿日期:2016-08-09 修回日期:2016-10-17 出版日期:2017-02-15 发布日期:2019-08-22
通讯作者: *jiangcj@ahau.edu.cn
作者简介:李梅,女,研究实习员,主要从事茶树分子生物学研究。
基金资助:
国家自然科学基金（31560220、31460216）、云南省人才培养计划（2015HB105）、茶树生物学与资源利用国家重点实验室开放基金（SKLTOF20150105）

Digital Gene Expression Analysis of Tea Flowers without Pistil at Three Development Stages

LI Mei¹, CHEN Linbo¹, TIAN Yiping¹, XIA Lifei¹, SONG Weixi¹, LIANG Mingzhi¹, JIANG Changjun^2,*

1. Tea Research Institute, Yunnan Academy of Agricultural Sciences, Yunnan Engineering Research Center of Tea Germplasm Innovation and Matching Cultivation, Menghai 666201, China;
2. State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei 230036, China

Received:2016-08-09 Revised:2016-10-17 Online:2017-02-15 Published:2019-08-22

摘要/Abstract

摘要： 以雌蕊缺失茶树花为试材,利用转录组、数字基因表达谱技术,研究了雌蕊缺失茶树花的花芽、花蕾、花3个时期相关基因的表达规律。结果发现,雌蕊缺失茶树花生长发育过程中进行着各种旺盛的生物合成和代谢活动。生长素信号转导途径的6个基因和ABCDE类识别基因的A类、C类和E类可能与茶树花的雌蕊缺失和雄蕊发育密切相关,并且基因调控过程较复杂;WUS基因中WUS2和WUS8下调可能调控了C类和E类基因,从而导致雌蕊的缺失;KNOX家族中,未检测到KNOXⅡ的同源基因,KNOX I类同源基因下调表达和缺失可能减弱了茶树花雌蕊心皮的启动和雌蕊边缘组织的生长。可以看出,本研究通过数字基因表达谱分析,初步了解了雌蕊缺失茶树花发育前后的网络途径,为后期对茶树花雌蕊缺失和雄蕊发育的研究,探明茶树花不育和性别决定基因的分子机制提供理论依据。

关键词: 茶树花, 雌蕊缺失, 数字基因表达谱, 转录组, 花器官调控

Abstract: The expression patterns of important genes in tea flowers without pistil at the flower bud, alabastrum and florescence stages was studied by the transcriptome and digital gene expression profiling technology. The results found that genes associating with biosynthesis and metabolic activities were highly expressed in tea flowers during the flowering process. Six genes involved in auxin signal transduction and the A、C and E genes of ABCDE model might be closely correlated with the stamen development and response to pistil deletion, which were under complex regulation. The down- regulation of WUS2 and WUS8 in WUS family might also regulate the C and E genes, and result in the deletion of pistil. In the KNOX gene family, none homologous gene of KNOXⅡ was detected. The down-regulation of 3 homologous genes of the KNOX I might weaken the initiation of carpels and the growth of marginal tissues. According to the digital gene expression analysis, a preliminary understanding of the network associating with the pistil deletion and stamen development in tea flower was obtained, which provides a theoretical basis for further research on pistil deletion, stamen development, molecular mechanism of sterility and sex determination in tea plant.

Key words: tea flowers, pistil deletion, digital gene expression profiling, transcriptome, floral organ regulation

中图分类号:

S571.1
Q52

李梅, 陈林波, 田易萍, 夏丽飞, 宋维希, 梁名志, 江昌俊. 雌蕊缺失茶树花3个发育期的数字基因表达谱分析[J]. 茶叶科学, 2017, 37(1): 97-107.

LI Mei, CHEN Linbo, TIAN Yiping, XIA Lifei, SONG Weixi, LIANG Mingzhi, JIANG Changjun. Digital Gene Expression Analysis of Tea Flowers without Pistil at Three Development Stages[J]. Journal of Tea Science, 2017, 37(1): 97-107.

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