茶树叶片精准测色装置构建及在稀有种质鉴别中的应用

茶叶科学 ›› 2017, Vol. 37 ›› Issue (2): 160-166.

茶树叶片精准测色装置构建及在稀有种质鉴别中的应用

孔祥瑞, 杨军, 王让剑

福建省农业科学院茶叶研究所/农业部茶树改良中心福建分中心,福建福安 355000

收稿日期:2016-12-26 修回日期:2017-02-06 出版日期:2017-04-15 发布日期:2019-08-22
作者简介:孔祥瑞,助理研究员,主要从事资源育种方面的研究。E-mail: xiangruikong@bioxxx.cn
基金资助:
福建省自然科学基金（2016J01118）、福建省省属公益类科研院所专项（2015R1012-7）

Establishment of Precise Color Measuring Device for Tea (Camellia sinesis L.) Leaf and Its Application in Identification of Rare Germplasm

KONG Xiangrui, YANG Jun, WANG Rangjian

Institute of Tea Reaearch, Fujian Academy of Agricultural Sciences/Fujian Sub-Center of National Tea Improvement Center, Ministry of Agriculture, Fu′an 355000, Chnina

Received:2016-12-26 Revised:2017-02-06 Online:2017-04-15 Published:2019-08-22

摘要/Abstract

摘要： 采用扫描仪、测色器ColorPix和RGB颜色系统建立测色装置,并利用在线工具ColorHexa将色差指标值转换成CIELab颜色体系参数,对绿、黄、紫色系典型茶树种质进行环向和径向测色法比较分析。结果表明,径向测定法采用单叶多点重复取值与环向测定法中环位取值具有相近叶色表征作用。通过对120个不同叶色种质的径向多点位重复测定和环向中环位测定,2种方法的明度L^*、色差指标值a^*和b^*显著正相关。但是,考虑到使用的简便性与分析效率,提出用环向中环位测定值定义叶色更为合适。对上述120个供试茶树种质的环向中环位叶色测定值构成的三维散点图进行分析,发现能够将紫红色种质与紫色系种质有效分离,更进一步证明该技术在特异叶色种质精准鉴别中具有实用价值。

关键词: 茶树, 叶色, 测色装置, 定量描述

Abstract: A tea leaf color measurement system was established by using the scanner, color picker tool ColorPix and RGB color rules. The value of color difference was converted into CIELab color system parameters by the online tool ColorHexa and then the leaf colors of Typical Green, Yellow and Purple tea Germplasms were comparative analyzed through circular direction and radial direction methods. The results showed that the single leaf multi - point repeat value in radial measurement and the mid-ring position value in circular method had similar leaf color characterization. The 120 tea germplasms were randomly selected and measured for the brightness L^* and chromatic components a^* and b^*. The values of lightness L^* and color difference a^* and b^* between the two methods were significantly and positively correlated. However, when the facilitation and the efficiency of analysis were taken into consideration, it is better to use the color value in the mid-ring position by circular direction method. When the leaf color values of 120 germplasms were measured in the mid-ring position to draw the three-dimensional scatter plots, the mauve color group was effectively separated from the purple germplasms, which further proved the efficiency of the method to accurately identify specific leaf color germplasm.

Key words: tea plant, leaf color, color measurement device, quantitative description

中图分类号:

S571.1
S326

孔祥瑞, 杨军, 王让剑. 茶树叶片精准测色装置构建及在稀有种质鉴别中的应用[J]. 茶叶科学, 2017, 37(2): 160-166.

KONG Xiangrui, YANG Jun, WANG Rangjian. Establishment of Precise Color Measuring Device for Tea (Camellia sinesis L.) Leaf and Its Application in Identification of Rare Germplasm[J]. Journal of Tea Science, 2017, 37(2): 160-166.

参考文献 22

[1]	蔡丽, 梁名志, 夏丽飞, 等. “紫娟”茶外观表象差异研究[J]. 西南农业学报, 2010, 23(3): 700-703.
[2]	季鹏章, 梁名志, 宋维希, 等. 茶树珍稀品种“紫娟”的叶片色素含量与叶色变化的关系研究[J]. 西南农业学报, 2011, 23(6): 1860-1862.
[3]	吴春燕, 须海荣. 不同茶树品种中黄酮苷含量的测定[J]. 茶叶科学, 2012, 32(2): 122-128.
[4]	曾超珍, 刘仲华. 安吉白茶阶段性白化机理的研究进展[J]. 分子植物育种, 2015, 13(12): 2905-2911.
[5]	杨伟丽, 何文斌, 张杰, 等. 论适制乌龙茶品种的特殊性状[J]. 茶叶科学, 1993, 13(2): 93-99.
[6]	段继华, 雷雨, 罗意, 等. 茶树芽叶颜色与发酵性能相关关系初探[J]. 湖南农业科学, 2014 (12): 68-71.
[7]	陈亮, 杨亚军, 虞富莲, 等. 茶树种质资源描述规范和数据标准[M]. 北京: 中国农业出版社, 2005: 11.
[8]	萧力争, 李勤, 谭正初, 等. 茶树紫色芽叶分级标准研究[J]. 云南农业大学学报: 自然科学版, 2008, 23(5): 668-672.
[9]	杨如兴, 孔祥瑞, 张磊, 等. 茶树品种(品系)芽叶色泽表型遗传数量分类研究[J]. 热带亚热带植物学报, 2016, 24(4): 444-451.
[10]	王开荣, 梁月荣, 李明, 等. 白化茶骨干亲本及其家系种质性状分析[J]. 茶叶, 2015, 41(3): 130-136.
[11]	Kim J, Kang S W, Pak C H, et al.Changes in leaf variegation and coloration of English ivy and polka dot plant under various indoor light intensities[J]. Hort Technology, 2012, 22(1): 49-55.
[12]	Kulkarni A H, Patil A R K. Applying image processing technique to detect plant diseases[J]. International Journal of Modern Engineering Research (IJMER), 2012, 2(5): 3661-3664.
[13]	Chaudhary P, Chaudhari A K, Cheeran A N, et al.Color transform based approach for disease spot detection on plant leaf[J]. International Journal of Computer Science and Telecommunications, 2012, 3(6): 65-70.
[14]	Yadav S P, Ibaraki Y, Gupta S D.Estimation of the chlorophyll content of micropropagated potato plants using RGB based image analysis[J]. Plant Cell, Tissue and Organ Culture (PCTOC), 2010, 100(2): 183-188.
[15]	白新祥, 胡可, 戴思兰, 等. 不同花色菊花品种花色素成分的初步分析[J]. 北京林业大学学报, 2006, 28(5): 84-89.
[16]	洪艳, 白新祥, 孙卫, 等. 菊花品种花色表型数量分类研究[J]. 园艺学报, 2012, 39(7): 1330-1340.
[17]	Byamukama R, Jordheim M, Kiremire B, et al.Anthocyanins from flowers of Hippeastrum cultivars[J]. Scientia Horticulturae, 2006, 109(3): 262-266.
[18]	Bintory M A, Ibaad M H, Subiya R, et al.Evaluation of dried dutch rose flowers for colour using colorimeter[J]. Trends in Biosciences, 2015, 8(11): 2996-2999.
[19]	刘东明, 廖述文. 茶树群体品种叶色与主要化学成分的个体差异[J]. 茶叶通讯, 1986(4): 10-14.
[20]	Yadav S P, Ibaraki Y, Gupta S D.Estimation of the chlorophyll content of micropropagated potato plants using RGB based image analysis[J]. Plant Cell, Tissue and Organ Culture (PCTOC), 2010, 100(2): 183-188.
[21]	Padmavathi K, Thangadurai K.Implementation of RGB and grayscale images in plant leaves disease detection-comparative study[J]. Indian Journal of Science & Technology, 2016, 9(6): 1-6.
[22]	Connolly C, Fleiss T.A study of efficiency and accuracy in the transformation from RGB to CIELAB color space[J]. IEEE Transactions on Image Processing, 1997, 6(7): 1046-1048.