采用茶汤有机试剂萃取分离,并分别经过反相C18柱层析纯化,制备出乙酸乙酯层、正丁醇层和酸性正丁醇层三部分茶红素。根据紫外可见光波扫描计算出色价,并研究了茶红素在不同条件下的稳定性。结果表明,30%、50%和70%甲醇梯度洗脱,纯化后3类茶红素液相检测发现无咖啡碱和茶黄素,仅有少量儿茶素杂质。纯化后色素色价分别为乙酸乙酯层128.04,正丁醇层91.04,酸性正丁醇层76.16。遮光处理,pH=3酸性条件,45℃以下贮藏,有利于茶红素的稳定性。
Abstract
In this study, thearubigins were extracted by organic extraction, and purified by reversed-phase C18 column chromatography to obtain three types of thearubigins, namely ethyl acetate, n-butanol and acid n-butanol extracts. The stability of thearubigins under different conditions was analyzed by scanning UV-Vis absorption wavelength based on color difference. The results showed that there was no caffeine and theaflavins, but only a small amount of catechins were detected in the three kinds of thearubigins after 30%, 50% and 70% methanol gradient elution. The color values of thearubigins after purification were 128.04 for ethyl acetate extracts, 91.04 for n-butanol extracts and 76.16 for acidic n-butanol extracts. Shading treatment, pH=3 acidic conditions and temperature below 45℃will facilitate the stability of thearubigins.
关键词
茶红素 /
色价 /
稳定性 /
柱层析
Key words
chromatography /
color values /
stability /
thearubigins
中图分类号：
TS272.5+2
Q946.84+1
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基金
国家茶叶产业技术体系红茶加工岗位（CARS-23）、中国农业科学院科技创新工程（CAAS-ASTIP-TRICAAS）、浙江省农产品化学与加工技术重点实验室/浙江省农业生物资源生化制造协同创新中心（2016KF0012）
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