本文以龙井43为对照,研究了茶丽纹象甲雌、雄成虫对不同品种茶苗的选择性和取食量差异,并比较了茶丽纹象甲取食诱导龙井43、黄旦、福云6号和丹桂4个品种茶苗所释放的4种重要萜烯类化合物的差异。结果表明,在有食源选择的情况下,茶丽纹象甲雌成虫在福云6号茶苗上的着落率和取食量都显著高于龙井43,而雌、雄两性对龙井43茶苗的取食量均显著高于黄旦;但是,在无食源选择的情况下,同一性别茶丽纹象甲对4个品种茶苗的取食量之间不具显著差异。与同一品种的健康茶苗相比,茶丽纹象甲取食均可显著诱导不同品种4种萜烯类物质的释放。茶丽纹象甲对不同品种茶苗为害相同时间,各品种萜烯类化合物的释放量有所差异,为害12βh,丹桂中芳樟醇的释放量和福云6号中DMNT、法呢烯的释放量显著高于龙井43;为害24βh,黄旦和丹桂中罗勒烯的释放量、福云6号中芳樟醇的释放量,以及黄旦、丹桂和福云6号中DMNT和法呢烯的释放量均显著高于龙井43。
Abstract
The discrepancies of host selection and food consumption of tea weevil in Longjing 43 and three other tea cultivars (namely Longjing 43, Huangdan, Fuyun 6 and Dangui) were investigated in the present study. The emission discrepancies of the four induced terpenoids were also determined in the four cultivars. The results showed that the landing rate and food consumption of tea weevil female adults in Fuyun 6 were significantly higher than those in Longjing 43 when there had food choice. While the food consumptions of both male and female tea weevil adults in Longjing 43 were significantly higher than those in Huangdan. However, when there had no food choice, food consumption of the same gender had no significant difference with each tea cultivar. When compared with the controls, the infestation of tea weevils dramatically induced the emission level of the four terpenoids in each tea cultivar. When the tea plants were infested by the tea weevils for 12 h, the amount of linalool in Dangui, the amount of (E)-4,8-dimethyl-1,3,7-nonatriene (DMNT), and farnesene in Fuyun 6 were significantly higher than those in LongJing 43. When the tea plants were infested by the tea weevils for 24 h, the amount of ocimene in Huangdan and Dangui, the amount of linalool in Fuyun 6, the amount of DMNT and the amount of farnesene in the three tea cultivars, Huangdan, Dangui and Fuyun 6, were significantly higher than those in Longjing 43.
关键词
茶丽纹象甲 /
茶树 /
品种 /
萜烯类化合物 /
诱导
Key words
Camellia Sinensis /
cultivar /
induced /
terpenoids /
Myllocerinus aurolineatus
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参考文献
[1] 娄永根, 程家安. 虫害诱导的植物挥发物基本特性、生态学功能及释放机制[J]. 生态学报, 2000, 20(6): 1097-1106.
[2] Clavijio MCA, Unsicker SB, Gershenzon J.The specificity of herbivore-induced plant volatiles in attracting herbivore enemies[J]. Trends Plant Sci, 2012, 17(5): 303-310.
[3] Bruce TJ, Wadhams LJ, Woodcock CM.Insect host location: a volatile situation[J]. TRENDS in Plant Science, 2005, 10(6): 269-274.
[4] Heil M.Indirect defence via tritrophic interactions[J]. New Phytologist, 2008, 178(1): 41-61.
[5] Xiao Y, Wang Q, Erb M, et al.Specific herbivore-induced volatiles defend plants and determine insect community composition in the field[J]. Ecology Letters, 2012, 15(10): 1130-1139.
[6] Saxena DB, Goswami BK.Nematicidal activity of some essential oils against Melneoidogyne incognita[J]. India Perfum, 1987, 31(2): 150-154.
[7] Bleeker PM, Mirabella R, Diergaarde PJ, et al.Improved herbivore resistance in cultivated tomato with the sesquiterpene biosynthetic pathway from a wild relative[J]. Proceedings of the National Academy of Sciences of the United States of America, 2012, 109(49): 20124-20129.
[8] Kollner TG, Lenk C, Schnee C, et al.Localization of sesquiterpene formation and emission in maize leaves after herbivore damage[J]. BMC Plant Biol, 2013, 13(1): 1-15.
[9] Bohlmann J.Pine terpenoid defences in the mountain pine beetle epidemic and in other conifer pest interactions: specialized enemies are eating holes into a diverse , dynamic and durable defence system[J]. Tree Physiol, 2012, 32(8): 943-945.
[10] Zhuang X, Kollner TG, Zhao N, et al.Dynamic evolution of herbivore-induced sesquiterpene biosynthesis in sorghum and related grass crops[J]. Plant Journal for Cell & Molecular Biology, 2012, 69(1): 70-80.
[11] Lou Y, Hua XY, Turlings TC, et al.Differences in induced volatile emissions among rice varieties results in differential attraction and parasitism of Nilaparvata lugens eggs by the parasitoid Anagrus nilaparvatae in the field[J]. Journal of Chemical Ecology, 2006, 32(1): 2375-2387.
[12] Gols R, Bullock JM, Dick M, et al.Smelling the wood from the trees: non-linear parasitoid responses to volatile attractants produced by wild and cultivated cabbage[J]. Journal of Chemical Ecology, 2011, 37(8): 795-807.
[13] Kariyat RR., Mauck KE, Moraes CMD, et al.Inbreeding compromises volatile signaling phenotypes and influences tritrophic interactions in horsenettle[J]. Ecology Letters, 2012, 15(4): 301-309.
[14] Dicke M, Takabayashi J, Posthumus MA, et al.Plant-phytoseiid interactions mediated by herbivore-induced plant volatiles: variation in production of cues and in responses of predatory mites[J]. Experimental & Applied Acarology, 1998, 22(6): 311-333.
[15] Kappers IF, Verstappen FWA, Luckerhoff LLP, et al.Genetic variation in jasmonic acid- and spider mite-induced plant volatile emission of cucumber accessions and attraction of the predator Phytoseiulus persimilis[J]. Journal of Chemical Ecology, 2010, 36(5): 500-512.
[16] Kappers IF, Hoogerbrugge H, Bouwmeester HJ, et al.Variation in herbivory-induced volatiles among cucumber (Cucumis sativus L.) varieties has consequences for the attraction of carnivorous natural enemies[J]. Journal of Chemical Ecology, 2011, 37(2): 150-160.
[17] Tahmasebi Z, Mohammadi H, Arimura G, et al.Herbivore-induced indirect defense across bean variety is independent of their degree of direct resistance[J]. Experimental & Applied Acarology, 2014, 63(2): 217-239.
[18] Sun XL, Wang GC, Cai XM, et al.The tea weevil, Myllocerinus aurolineatus, is attracted to volatiles induced by conspecifics[J]. Journal of Chemical Ecology, 2010, 36(4): 388-395.
[19] Sun XL, Wang GC, Gao Y, et al.Screening and field evaluation of synthetic volatile blends attractive to adults of the tea weevil, Myllocerinus aurolineatus[J]. Chemoecology, 2012, 22(4): 229-237.
[20] 刘丰静, 李慧玲, 王定锋, 等. 茶丽纹象甲取食习性与防治指标研究[J]. 茶叶学报, 2015, 56(1): 45-50.
[21] 边文波, 王国昌, 龚一飞, 等. 十九种植物精油对茶丽纹象甲成虫的驱避和拒食活性[J]. 应用昆虫学报, 2012, 49(2): 496-502.
[22] Clavijio MCA, Gershenzon J.Little peaks with big effects: establishing the role of minor plant volatiles in plant-insect interactions[J]. Plant Cell and Environment, 2014, 37(8): 1836-1844.
[23] Dicke M, Baldwin LT.The evolutionary context for herbivore-induced plant volatiles: beyond the ‘cry for help’[J]. Trends in Plant Science, 2009, 15(3): 167-175.
[24] Allmann S, Baldwin LT.Insects betray themselves in nature to predators by rapid isomerization of green leaf volatiles[J]. Science, 2010, 329(5995): 1075-1078.
[25] Erbilgin N, Krokene P, Christiansen E, et al.Exogenous application of methyl jasmonate elicits defenses in Norway spruce (Picea abies) and reduces host colonization by the bark beetle Ips typographus[J]. Oecologia, 2006, 148(3): 426-436.
[26] Weaver DK, Buteler M, Hofland ML, et al.Cultivar preferences of ovipositing wheat stem sawflies as influenced by the amount of volatile attractant[J]. Journal of Economic Entomology, 2009, 102(3): 1009-1017.
[27] Xin ZJ, Li XW, Bian L, et al.Tea green leafhopper, Empoasca vitis, chooses suitable host plants by detecting the emission level of (3Z)-hexenyl acetate[J]. Bulletin of Entomological Research, 2016. doi: 10.1017/S000748531600064X.
[28] Sun XL, Wang GC, Gao Y, et al.Volatiles emitted from tea plants infested by Ectropis obliqua larvae are attractive to conspecific moths[J]. Journal of Chemical Ecology, 2014, 40(10): 1080-1089.
[29] Cai XM, Sun XL, Dong WX, et al.Herbivore species, infestation time, and herbivore density affect induced volatiles in tea plants[J]. Chemoecology, 2014, 24(1): 1-14.
[30] Bernasconi ML, Turlings TCJ, Ambrosetti L, et al.Herbivore induced emissions of maize volatiles repel the corn leaf aphid, Rhopalosiphum maidis[J]. Entomologia Experimentalis et Applicata, 1998, 87(2): 133-142.
[31] De Moraes CM, Mescher MC, Tumlinson JH.Caterpillar-induced nocturnal plant volatiles repel conspecific females[J]. Nature, 2001, 410(29): 577-580.
[32] Kessler A, Baldwin IT.Defensive function of herbivore-induced plant volatile emissions in nature[J]. Science, 2001, 291(16): 2141-2144.
[33] 赵冬香. 茶树-假眼小绿叶蝉-蜘蛛间化学、物理通讯机制的研究[D]. 杭州: 浙江大学, 2001: 26-50
[34] Kiran SR, Reddy AS, Devi PS, et al.Insecticidal, antifeedant and oviposition deterrent effects of the essential oil and individual compounds from leaves of Chloroxylon swietenia DC[J]. Pest Management Science, 2006, 62(62): 1116-1121.
[35] 金珊, 孙晓玲, 陈宗懋, 等. 不同茶树品种对假眼小绿叶蝉的抗性[J]. 中国农业科学, 2012, 45(2): 255-265.
基金
公益性行业（农业）科研专项经费（201403030）、浙江省科技厅公益技术研究农业项目资助（2015C32081）、浙江省自然科学基金项目资助（LQ14C140001）、浙江省“151”人才工程资助项目
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