EGCG纳米硒的稳定性及其对硒吸收利用的影响

摘要/Abstract

摘要： 使用维生素C（Vitamin C,Vc）作为氧化还原剂和(-)-表没食子儿茶素没食子酸酯（EGCG）作为分散剂,成功制备了纳米硒（EGCG-SeNPs）。使用透射电子显微镜（TEM）和激光粒度分析仪电动电势仪（Zetasizer）测定的EGCG-SeNPs是平均直径为(35±0.12)βnm、Zeta电位为–0.05βmV的球形颗粒。EGCG-SeNPs在pH1.0的强酸或70℃高温环境下发生聚集,其颗粒大小增加大约10倍。而且,EGCG-SeNPs中的EGCG作为分散剂具有良好的稳定性。通过分别灌胃25、50、100βμg·kg^-1（以含Se量计算）的亚硒酸钠和EGCG-SeNPs发现,肝脏和肾脏中的硒含量在50、100βμg·kg^-1的硒处理后均显著增加;在血清、肝脏和肾脏中,所有处理组的谷胱甘肽过氧化物酶（GPx）活性均显著增加,且具有剂量依赖效应。然而,在相同剂量的亚硒酸钠和EGCG-SeNPs处理组中,硒含量和GPx活性之间没有显著差异。因此可以得出,Vc作为氧化还原剂制备的EGCG-SeNPs可能具有和亚硒酸钠相似的生物利用度。

关键词: (-)-表没食子儿茶素-3-没食子酸酯, 硒, 纳米颗粒, 生物利用度, 谷胱甘肽过氧化物酶活性

Abstract: (-)-epigallocatechin-3-gallate (EGCG) dispersed Selenium nanoparticles (EGCG-SeNPs) were prepared using vitamin C (Vc) and EGCG as the redox agent and dispersant. Characteristics of EGCG-SeNPs, which were determined using transmission electron microscope (TEM) and zetasizer, were spherical in shape with a mean diameter of (35±0.12)βnm and -0.05βmV zeta potential. The particles were aggregated in strong acid and high temperature conditions (pH1.0 and 70℃), with the particle size increased by about 10 times. And, EGCG in EGCG-SeNPs had good stability as a dispersant. With the administration of 25, 50 and 100βμg·kg^-1 (Calculated with selenium content), selenium content in liver and kidney of 50 and 100βμg·kg^-1 sodium selenite and EGCG-SeNPs treated mice were significantly increased. Glutathione peroxidase (GPx) activity in serum, liver and kidney of all the treatment groups were significantly increased in a dose-dependent manner. However, there was no significant difference between sodium selenite and EGCG-SeNPs at the same dose on selenium content and GPx activity. Hence, it can be concluded that EGCG-SeNPs synthesized using Vc as the redox agent might have the similar bioavailability to sodium selenite.

Key words: (-)-epigallocatechin-3-gallate, selenium, nanoparticle, bioavailability, glutathione peroxidase activity

中图分类号:

王乐, 李欢, 李嘉豪, 陈暄, 黎星辉, 孙康. EGCG纳米硒的稳定性及其对硒吸收利用的影响[J]. 茶叶科学, 2017, 37(4): 373-382.

WANG Le, LI Huan, LI Jiahao, CHEN Xuan, LI Xinghui, SUN Kang. The Evaluation of the Stability of EGCG-Selenium Nanoparticles and Its Effect on Selenium Absorption and Utilization[J]. Journal of Tea Science, 2017, 37(4): 373-382.

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