取1、3、7年寿眉（白茶）制备白茶提取物（White tea extracts, WTEs）。在对雄性C57BL/6J小鼠喂饲高脂饲料的同时给予WTEs干预,诱导建立预肥胖模型。观察小鼠体质量增量、摄食量、脂肪和肝脏组织病理学变化,统计脂肪湿重,测定血脂及血转氨酶水平。qPCR测定小鼠肝脏中脂代谢相关基因表达。综合评价WTEs预防小鼠肥胖功效及其安全性,并对其机制进行探究。结果显示,WTEs在无肝毒性和生长抑制作用条件下,能抑制脂肪细胞分化和脂肪的过度积累,还能下调SREBP-1c、FAS和ACC1的转录水平,抑制肝脏脂肪酸合成和脂肪过度积累,有效预防肥胖和脂肪肝的发生。高剂量下,WTEs预防肥胖功效随白茶年份的增加而显著降低。
Abstract
White tea extracts (WTEs) had been prepared from Shoumei white tea stored for 1, 3 and 7 years, respectively. Male mice (C57BL/6J) were fed with high-fat diet and supplemented with WTE to establish the anti-obesity efficacy model. Body weight gain, food intake, the pathobiology changes of the liver and adipose tissues, serum lipids profiles and differential expression profiles of lipid metabolism-related genes of liver in mice were investigated. The results showed that WTEs inhibited adipocyte differentiation and lipid excessive accumulation in adipose tissues, inhibited fatty acid synthesis and lipid excessive accumulation in liver by ameliorating the expression levels of lipid metabolism-related genes via down-regulation of the mRNA expression level of SREBP-1c, FAS and ACC1, thus depressing the increase of body weight and adipose tissues induced by high-fat diet. The preventing obesity efficacy of Shoumei white tea in high dosage was reduced with the increase of the storage years..
关键词
安全性 /
白茶 /
预防肥胖
Key words
anti-obesity /
safety /
white tea
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1] 中国国家标准化管理委员会. GB/T 30766—2014 茶叶分类[S]. 北京: 中国标准出版社, 2014: 2.
[2] 叶乃兴. 白茶、白叶茶与白毛茶[J]. 福建茶叶, 2010, 10: 44-46.
[3] 刘国根, 罗泽民, 邱冠周, 等. 茶叶中自由基的研究[J]. 湖南农业大学学报: 自然科学版, 1999, 25(4): 290-292.
[4] 中华人民共和国国家质量监督检验检疫总局. GB/T 8313-2008. 中华人民共和国国家标准-茶叶中茶多酚和儿茶素含量的检测方法[S]. 北京: 中国标准出版社, 2008-05-04.
[5] 中华人民共和国国家质量监督检验检疫总局. GB/T 8312-2013. 中华人民共和国国家标准-茶-咖啡碱测定[S]. 北京: 中国标准出版社, 2013-12-31.
[6] 马陶陶, 张群林, 李俊, 等. 三氯化铝比色法测定中药总黄酮方法的探讨[J]. 时珍国医国药, 2008, 19(1): 54-56.
[7] 王蝶. 茶叶对肥胖大鼠的减肥作用及机制研究[D]. 长沙: 湖南农业大学, 2012: 10-11.
[8] 黄继汉, 黄晓晖, 陈志扬, 等. 药理试验中动物间和动物与人体间的等效剂量换算[J]. 中国临床药理学与治疗学, 2004, 9(9): 1069-1072.
[9] 贺争鸣. 实验动物管理与使用指南[M]. 北京: 科学出版社, 2016: 142-150.
[10] 章金涛. 医学实验动物学[M]. 郑州: 郑州大学出版社, 2014: 385-387.
[11] Livak K J, Schmittgen T D.Analysis of relative gene expression data using real-timequantitative PCR and the 2(-Delta Delta C(T)) Method[J]. Methods, 2001, 25(4): 402-408.
[12] Kautzky W A, Lemmens G R.Obesity and diabetes[M]//Regitz Zagrosek V. Sex and gender differences in pharmacology. Heidelberg: Springer, 2013: 307-340.
[13] Aherne S A, O'Brien N M. Dietary flavonols: chemistry, food content, and metabolism[J]. Nutrition, 2002, 18(1): 75-81.
[14] Gondoin A, Grussu D, Stewart D, et al.White and green tea polyphenols inhibit pancreatic lipase in vitro[J]. Food Research International, 2010, 43(5): 1537-1544.
[15] Martins F, Noso TM, Porto VB, et al.Maté tea inhibits in vitro pancreatic lipase activity and has hypolpidemic effect on high-fat diet-induced obese mice[J]. Obesity, 2009, 18(1): 42-47.
[16] Westerterp-Plantenga M S, Mattes R. Green tea catechins, caffeine and body-weight regulation[J]. Physiology & Behavior, 2010, 100(1): 42-46.
[17] 王丽娟, 李晓蓉, 李宇航, 等. 高脂饲料对C57BL/6J小鼠血脂与转氨酶的动态影响[J]. 2007, 17(10): 572-576.
[18] Nishiumi S, Bessyo H, Kubo M, et al.Green and black tea suppress hyperglycemia andInsulin resistance by retaining the expression of glucose transporter 4 in muscle of high-fat diet-fed C57BL/6J mice[J]. Journal of Agricultural & Food Chemistry, 2010, 58(24): 12916-12923.
[19] Byun J K, Yoon B Y, Jhun J Y, et al.Epigallocatechin- 3-gallate ameliorates both obesity and auto inflammatory arthritis aggravated by obesity by altering the balance among CD4+ T-cellsubsets[J]. Immunology Letters, 2014, 157(12): 51-58.
[20] Mousumi B, Joshua D. Lambert J, et al.The major green tea polyphenol, (-)-epigallocatechin-3-gallate, inhibits obesity, metabolic syndrome, and fatty liver disease in high-fat-fed mice[J]. Journal of Nutrition, 2008, 138(9): 1677-1683.
[21] Chen YK, Cheung C, Reuhl KR, et al.Effects of green tea polyphenol (-)-epigallocatechin-3-gallate on a newly developed high-fat/western-style Ddiet-induced obesity and metabolic syndrome in mice[J]. Journal of Agricultural & Food Chemistry, 2011, 59(21): 11862-11871.
[22] Murase, TNagasawa, ASuzuki, et al. Beneficial effects of tea catechins on diet-induced obes-ity: stimulation of lipid catabolism in the liver[J]. International Journal of Obesity, 2002, 26(11): 1459-1464.
[23] 陈国良, 刘立伟, 谢爽, 等. 高密度脂蛋白胆固醇代谢及其对冠心病影响的研究进展[J]. 心血管病学进展, 2010, 31(3): 360-363.
[24] Rizzo M, Otvos J, Nikolic D, et al.Subfractions and subpopulations of HDL: an update[J]. Current Medicinal Chemistry, 2014, 21(25): 2881-2891.
[25] Kozarsky K F, Donahee M H, Rigotti A, et al.Overexpression of the HDL receptor SR-BIalters plasma HDL and bile cholesterol levels[J]. Nature, 1997, 387(6631): 414-417.
[26] Rigotti A, Trigatti B L, Penman M, et al.A targeted mutation in the murine gene encoding the high density lipoprotein (HDL) receptor scavenger receptor class B type I reveals its key role in HDL metabolism[J]. Proceedings of the National Academy of Sciences of the United States of America, 1997, 94(23): 12610-12615.
[27] 杨永青. 脂肪细胞分化及其调控机制[M]. 北京: 中国农业科学技术出版社, 2014: 12-14.
基金
北京食品营养与人类健康高精尖创新中心开放基金项目
PDF(1198 KB)
浙公网安备 33019902000101号