福建茶树种质资源群体结构及遗传差异

杨军; 张力岚; 张雯婧; 陈林海; 郑国华; 李毅晶; 王让剑

doi:10.13305/j.cnki.jts.2023.06.006

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茶叶科学 ›› 2023, Vol. 43 ›› Issue (6) : 769-783. DOI: 10.13305/j.cnki.jts.2023.06.006

研究报告

福建茶树种质资源群体结构及遗传差异

杨军¹, 张力岚¹, 张雯婧², 陈林海³, 郑国华⁴, 李毅晶⁵, 王让剑^1,*

作者信息 +

Population Structure and Genetic Differences of Tea Germplasm Resources in Fujian

YANG Jun¹, ZHANG Lilan¹, ZHANG Wenjing², CHEN Linhai³, ZHENG Guohua⁴, LI Yijing⁵, WANG Rangjian^1,*

Author information +

文章历史 +

摘要

利用38对SSR荧光引物对208个福建茶树种质资源的群体遗传结构、遗传多态性、遗传分化、基因流、分子方差进行分析,并调查其叶片性状。结果表明,208个福建茶树资源的Nei’s遗传多样性指数为0.674,Shannon’s信息指数为1.444;叶面积与叶片长宽比平均值分别为27.442 cm²和2.516;福建茶树种质资源的遗传变异主要来源于个体间遗传变异。经群体结构分析将供试材料分为8个群体,群体a、b、f、h内材料来源单一,群体c、d、e、g内材料来源复杂,不同地点间茶树群体遗传背景相似。群体a、群体b、群体e内共有40个福建茶树品种,群体a内主要为适制绿茶品种,群体b内主要为适制乌龙茶品种,群体e内代表性品种为适制绿茶品种,群体a、群体b、群体e的群体属性与适制茶类存在一定相关性;群体c内包含南靖县、云霄县与平和县资源,地理位置相对较近,群体属性与地理来源相关。群体g与群体e群体间基因流为6.321,表明群体间基因交流频繁;群体相似系数聚类显示群体d与群体b亲缘关系较近。群体h与其他群体间遗传分化明显,叶面积、叶齿数性状特征差异显著（P<0.05）,群体f与其他群体间亲缘关系较远,叶齿数、叶脉对数性状特征差异显著（P<0.05）,说明群体h和群体f都具有一定的独特性,需进一步鉴定。本研究结果为福建茶树种质资源的鉴定、筛选与利用提供一定的参考。

Abstract

The population genetic structure, genetic diversity, genetic differentiation, gene flow, molecular variance of 208 tea germplasm resources in Fujian were studied by using 38 pairs of SSR fluorescent primers, and the leaf traits were also investigated. The results show that the Nei’s genetic diversity index and Shannon’s information index of 208 tea resources in Fujian were 0.674 and 1.444, respectively. The average values of leaf area and length-width ratio were 27.442 cm² and 2.516, respectively. The genetic variation of tea germplasm in Fujian were mainly originated from individual genetic variation. The test materials were divided into 8 groups by the structure software analysis. The material sources in groups a, b, f, and h were single, while the material sources in groups c, d, e, and g were complex. The genetic background of tea population in different regions was similar. There were a total of 40 tea cultivars from Fujian in groups a, b, and e. Group a was mainly cultivars suitable for processing green tea. Group b was mainly cultivars suitable for processing oolong tea. The representative cultivars within group e were suitable for processing green tea. There is a certain correlation between the group attributes of group a, group b, group e and the suitable tea cultivars. Group c included the resources from Nanjing county, Yunxiao county and Pinghe county, with relatively close geographical locations. The group attributes are related to geographical sources. The gene flow value between group g and group e was 6.321, indicating frequent gene exchange between groups. The clustering of group similarity coefficients shows that group d and group b were closely related. The genetic differentiation between group h and other groups was obvious, and the characters of leaf area and leaf serration number were significantly different (P<0.05). The genetic relationship between group f and other groups was relatively distant, and there were significant differences in the characteristics of leaf serration number and leaf vein logarithm (P<0.05), showing that both group h and group f had certain uniqueness and need further identification. These research results provided a certain reference for the identification, screening, and utilization of tea germplasm resources in Fujian.

导出引用

杨军, 张力岚, 张雯婧, 陈林海, 郑国华, 李毅晶, 王让剑. 福建茶树种质资源群体结构及遗传差异[J]. 茶叶科学. 2023, 43(6): 769-783 https://doi.org/10.13305/j.cnki.jts.2023.06.006

YANG Jun, ZHANG Lilan, ZHANG Wenjing, CHEN Linhai, ZHENG Guohua, LI Yijing, WANG Rangjian. Population Structure and Genetic Differences of Tea Germplasm Resources in Fujian[J]. Journal of Tea Science. 2023, 43(6): 769-783 https://doi.org/10.13305/j.cnki.jts.2023.06.006

中图分类号： S571.1 S324

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