江西茶树叶部病害调查与病原真菌初步鉴定

李必聪, 江红, 黄纪刚, 张礼萍, 吴韡, 姚甄业, 石正海, 纪羽, 张晓阳

茶叶科学 ›› 2026, Vol. 46 ›› Issue (4) : 667-678.

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茶叶科学 ›› 2026, Vol. 46 ›› Issue (4) : 667-678. DOI: 10.13305/j.cnki.jts.2026.04.003
研究报告

江西茶树叶部病害调查与病原真菌初步鉴定

  • 李必聪1,2,4, 江红1, 黄纪刚3, 张礼萍1, 吴韡1,4, 姚甄业1,4, 石正海1,4, 纪羽1,4, 张晓阳2,3,*
作者信息 +

Investigation of Tea Leaf Diseases and Preliminary Identification of Pathogenic Fungi in Jiangxi Province

  • LI Bicong1,2,4, JIANG Hong1, HUANG Jigang3, ZHANG Liping1, WU Wei1,4, YAO Zhenye1,4, SHI Zhenghai1,4, JI Yu1,4, ZHANG Xiaoyang2,3,*
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文章历史 +

摘要

为明确江西茶树叶部病害及其病原真菌发生情况,制定行之有效的绿色防控对策。2019—2022年对江西省61个茶园进行茶树叶部病害实地调查,采集病叶,对病原真菌进行分离纯化后分别进行形态学观察和基于内在转录间隔区(Internal transcribed spacer,ITS)序列的分子鉴定。研究发现,为害江西茶树的叶部病害有25种,初步分离获得258株茶树叶部病原真菌,分布在19个属。叶部病害病原真菌中以刺盘孢属(Colletotrichum)和类拟盘多毛孢菌(Pestalotiopsis-Like)分离率最高,是叶部病害的主要病原真菌。链格孢属(Alternaria)、葡萄座腔菌属(Botryosphaeria)、间座壳属(Diaporthe)、镰孢属(Fusarium)、黑孢属(Nigrospora)、拟茎点霉属(Phomopsis)和叶点霉属(Phyllosticta)7个属的真菌在多个茶园病叶中被分离到,为次要病原真菌。茶饼病和茶白星病在高海拔茶区流行,茶煤病在个别茶园为害较重。

Abstract

To clarify the occurrence of tea leaf diseases and their pathogenic fungi in Jiangxi Province, and to formulate effective green prevention and control measures. From 2019 to 2022, the field investigation of tea leaf diseases was carried out in 61 tea gardens in Jiangxi Province. The diseased leaves were collected, and after isolation and purification of the pathogens, morphological observation and molecular identification based on internal transcribed spacer (ITS) sequences were carried out respectively. It was found that there were 25 kinds of tea leaf diseases in Jiangxi Province, and 258 strains of pathogenic fungi were isolated from tea leaves, which were distributed in 19 genera. Among the pathogenic fungi of leaf diseases, Colletotrichum and Pestalotiopsis-like fungi had the highest isolation rate and were the main pathogens of leaf diseases. A total of 7 genera, including Alternaria, Botryosphaeria, Diaporthe, Fusarium, Nigrospora, Phomopsis and Phyllosticta were isolated from diseased leaves of several tea gardens and were identified as secondary pathogens. Tea blister blight and tea white scab are prevalent in high-altitude tea areas, and tea sooty mould is more serious in individual tea gardens.

关键词

茶树 / 叶部病害调查 / 病原真菌鉴定

Key words

tea plant / investigation of foliar disease / identification of pathogenic fungi

引用本文

导出引用
李必聪, 江红, 黄纪刚, 张礼萍, 吴韡, 姚甄业, 石正海, 纪羽, 张晓阳. 江西茶树叶部病害调查与病原真菌初步鉴定[J]. 茶叶科学. 2026, 46(4): 667-678 https://doi.org/10.13305/j.cnki.jts.2026.04.003
LI Bicong, JIANG Hong, HUANG Jigang, ZHANG Liping, WU Wei, YAO Zhenye, SHI Zhenghai, JI Yu, ZHANG Xiaoyang. Investigation of Tea Leaf Diseases and Preliminary Identification of Pathogenic Fungi in Jiangxi Province[J]. Journal of Tea Science. 2026, 46(4): 667-678 https://doi.org/10.13305/j.cnki.jts.2026.04.003
中图分类号: S571.1    S435.711   

参考文献

[1] 陈宗懋. 新时代中国茶产业的创新与发展[J]. 农学学报, 2018, 8(1): 80-84.
Chen Z M.Innovation and development of China tea industry in new era[J]. Journal of Agriculture, 2018, 8(1): 80-84.
[2] 孙永明, 叶川, 张昆, 等. 江西省茶叶产业发展分析报告[J]. 江西农业学报, 2016, 28(8): 110-114.
Sun Y M, Ye C, Zhang K, et al.Report on development of tea industry in Jiangxi Province[J]. Acta Agriculturae Jiangxi, 2016, 28(8): 110-114.
[3] 蔡晓明, 周凌云, 修春丽, 等. 茶树植保研究“十四五”进展及“十五五”发展方向[J]. 中国茶叶, 2026, 48(2): 1-10.
Cai X M, Zhou L Y, Xiu C L, et al.Research progress on tea plant protection during the 14th Five-Year Plan Period and development direction in the 15th Five-Year Plan Period[J]. China Tea, 2026, 48(2): 1-10.
[4] 涂一怡, 张幼, 徐婷, 等. 基于环介导等温扩增技术检测Colletotrichum camelliae[J]. 茶叶科学, 2025, 45(5): 770-782.
Tu Y Y, Zhang Y, Xu T, et al.Loop-mediated isothermal amplification-based detection of Colletotrichum camelliae[J]. Journal of Tea Science, 2025, 45(5): 770-782.
[5] 唐美君, 郭华伟, 姚惠明, 等. 近30年我国茶树新增病害名录[J]. 中国茶叶, 2019, 41(10): 14-15.
Tang M J, Guo H W, Yao H M, et al.List of new tea plant diseases in China in recent 30 years[J]. China Tea, 2019, 41(10): 14-15.
[6] 肖强. 茶树病虫和天敌目录[M]. 北京: 中国农业出版社, 2020: 1-19.
Xiao Q.Catalogue of tea plant diseases, pests and natural enemies [M]. Beijing: China Agricultural Press, 2020: 1-19.
[7] Yin Q X, Jiang S L, Li D X, et al. First report of Epicoccum nigrum causing brown leaf spot in tea in Guizhou Province, China [J]. Plant Disease, 2021, 106(1): 321. https://doi.org/10.1094/PDIS-04-21-0815-PDN.
[8] Cheng L, Thangaraj K, Deng C, et al. Phyllosticta capitalensis causes leaf spot on tea plant (Camellia sinensis) in China [J]. Plant Disease, 2019, 103(11): 2964. https://doi.org/10.1094/PDIS-04-19-0768-PDN.
[9] Wang X, Yin Q X, Jiang S L, et al. First report of Didymella bellidis causing tea leaf spot in China [J]. Plant Disease, 2019, 104(4): 1254. https://doi.org/10.1094/PDIS-08-19-1782-PDN.
[10] Lü W Y, Yu Y T, Zhong X F, et al.Identification and characterization of Cladosporium species associated with tea plants (Camellia sinensis) in China[J]. Plant Pathology, 2023, 72(5): 868-880.
[11] 程宇豪, 黄小贞, 张金峰, 等. 贵州茶叶斑点病病原系统发育学分析与生物学特性[J]. 分子植物育种, 2022, 20(13): 4468-4476.
Cheng Y H, Huang X Z, Zhang J F, et al.Phylogenetic analysis and biological characteristics of tea leaf spot disease in Guizhou[J]. Molecular Plant Breeding, 2022, 20(13): 4468-4476.
[12] 夏天帅, 甘艺贤, 王庭博, 等. 水城县茶树常见病害病原分离及鉴定[J]. 现代农业科技, 2020(12): 135-137.
Xia T S, Gan Y X, Wang T B, et al.Isolation and identification of common pathogens of tea plant diseases in Shuicheng County[J]. Modern Agricultural Science and Technology, 2020(12): 135-137.
[13] 卢声洁, 赵兴丽, 罗林丽, 等. 一株茶轮斑病病原菌的分离鉴定及致病力[J]. 茶叶通讯, 2021, 48(2): 253-258.
Lu S J, Zhao X L, Luo L L, et al.Isolation, identification of a pathogen of tea gray blight and its pathogenicity[J]. Journal of Tea Communication, 2021, 48(2): 253-258.
[14] 杨芳, 江冰冰, 雷金梅, 等. 茶树叶斑病病原菌的分离与鉴定[J]. 茶叶科学, 2025, 45(2): 266-272.
Yang F, Jiang B B, Lei J M, et al.Isolation and identification of the pathogen causing leaf spot in tea plants[J]. Journal of Tea Science, 2025, 45(2): 266-272.
[15] 马思宇, 龙丽雪, 李子龙, 等. 云南大叶种茶树炭疽病病原分离鉴定及生防菌筛选[J]. 茶叶科学, 2026, 46(1): 89-100.
Ma S Y, Long L X, Li Z L, et al.Isolation and identification of the pathogen causing anthracnose on yunnan large-leaf tea plants and screening of antagonistic bacteria[J]. Journal of Tea Science, 2026, 46(1): 89-100.
[16] 孙晓玲. 中国重要茶树叶部病害的研究现状及展望[J]. 中国茶叶, 2016, 38(12): 12-13.
Sun X L.Research status and prospect of important tea leaf diseases in China[J]. China Tea, 2016, 38(12): 12-13.
[17] 汪荣灶, 王林志, 万玲. 江西茶树病害种类及危害情况调查简记[C]//中国茶叶学会, 台湾茶协会. 第七届海峡两岸茶叶学术研讨会论文集(摘要), 南昌: [出版者不详], 2012: 67.
Wang R Z, Wang L Z, Wan L.The investigation of the species and the hazards profiles of the tea tree diseases in Jiangxi Province [C]//China Tea Science Society, Taiwan Tea Association. Abstracts of the 7th Cross-Strait Tea Academic Symposium. Nanchang: [s.n.], 2012: 67.
[18] 方中达. 植病研究方法[M]. 北京: 中国农业出版社, 1998: 10-124.
Fang Z D.Plant disease research methods [M]. Beijing: China Agricultural Publishing House, 1998: 10-124.
[19] 王振华. 一种新茶树病害鉴定及其检测技术研究[D]. 武汉: 华中农业大学, 2017: 21.
Wang Z H.Studies on identification of a new tea disease and its detection technology [D]. Wuhan: Huazhong Agricultural University, 2017: 21.
[20] 陈宗懋, 陈雪芬. 世界茶树病原名录[J]. 茶叶科学, 1988(2): 65-76.
Chen Z M, Chen X F.World tea plant pathogen list[J]. Journal of Tea Science, 1988(2): 65-76.
[21] 陈宗懋, 陈雪芬. 世界茶树病原名录(续)[J]. 茶叶科学, 1989(1): 73-88.
Chen Z M, Chen X F.World tea plant pathogen list (continued)[J]. Journal of Tea Science, 1989(1): 73-88.
[22] 王科, 刘芳, 蔡磊. 中国农业植物病原菌物常见种属名录[J]. 菌物学报, 2022, 41(3): 361-386.
Wang K, Liu F, Cai L.A name list of common agricultural phytopathogenic fungi in China[J]. Mycosystema, 2022, 41(3): 361-386.
[23] 杨友联. 中国贵州、云南、广西炭疽菌属真菌多基因分子系统学研究[D]. 武汉: 华中农业大学, 2010: 7-10.
Yang Y L.Multi-locus phylogeny of Colletotrichum species in Guizhou, Yunnan and Guangxi, China [D]. Wuhan: Huazhong Agricultural University, 2010: 7-10.
[24] Wang Y, Hao X, Wang L, et al. Diverse Colletotrichum species cause anthracnose of tea plants (Camellia sinensis (L.) O. Kuntze) in China [J]. Scientific Reports, 2016, 6(1): 35287. https://doi.org/10.1038/srep35287.
[25] 施云龙. 茶树抗炭疽病和抗冻机制及评价研究[D]. 杭州: 浙江大学, 2020: 45-49.
Shi Yunlong.Studies on molecular mechanism of tea plant against anthracnose infection and assessment of freezing tolerance of tea cultivars [D]. Hangzhou: Zhejiang University, 2020: 45-49.
[26] Bao X T, Dharmasena D S P, Li D X, et al. First report of Epicoccum sorghinum causing leaf spot on tea in China [J]. Plant Disease, 2019, 103(12): 3282. https://doi.org/10.1094/PDIS-06-19-1296-PDN.
[27] Liu Y J, Tang Q, Fang L. First report of Nigrospora sphaerica causing leaf blight on Camellia sinensis in China [J]. Plant Disease, 2015, 100(1): 221. https://doi.org/10.1094/PDIS-04-15-0493-PDN.
[28] Dutta J, Gupta S, Thakur D, et al. First report of Nigrospora leaf blight on tea caused by Nigrospora sphaerica in India [J]. Plant Disease, 2014, 99(3): 417. https://doi.org/10.1094/PDIS-05-14-0545-PDN.
[29] Zhou L X, Xu W X. First report of Alternaria alternata causing leaf spots of tea (Camellia sinensis) in China [J]. Plant Disease, 2014, 98(5): 697. https://doi.org/10.1094/PDIS-10-13-1086-PDN.
[30] Thangaraj K, Deng C, Cheng L, et al. Inhibition mechanism of caffeine in tea pathogenic fungi Botryosphaeria dothidea and Colletotrichum gloeosporioides [J]. Research Square, 2020. https://api.semanticscholar.org/CorpusID:234693680.
[31] Tang Z, Zhu J, Song Q, et al. Identification and pathogenicity of Fusarium spp. associated with tea wilt in Zhejiang Province, China [J]. BMC Microbiology, 2024, 24(1): 38. https://doi.org/10.1186/s12866-023-03174-4.

基金

江西省教育厅科学技术研究项目(GJJ2405804); 江西省茶叶产业技术体系建设专项(JXARS-06); 江西省农牧渔业科研课题(赣农厅办函〔2024〕12号-20)

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