冷驯化和ABA对茶树抗寒力及其体内脯氨酸含量的影响

doi:10.13305/j.cnki.jts.2004.03.006

摘要/Abstract

摘要： 本文研究了冷驯化和ABA对茶树抗寒力及其体内脯氨酸含量的影响,结果表明：通过冷驯化或者ABA处理,茶树经历了驯化再到脱驯化的过程,相应地茶树的抗寒力也发生了很大的变化,由驯化前的几乎为0逐渐增大,而随着脱驯化的进行又迅速恢复到驯化前的水平。低温驯化的临界温度为7℃左右,脱驯化的临界温度为9℃左右。人工低温驯化和ABA处理,茶树抗寒力的提高幅度没有自然冷驯化下的大。对三种处理下茶树体内脯氨酸含量的变化的研究发现,冷驯化中茶树体内脯氨酸含量变化是对外界条件变化的一种综合反应,很难说明单一的脯氨酸含量与抗寒力之间的因果关系。

关键词: 茶学, 茶树[Camellia sinensis(L.) O. Kuntze], 冷驯化, 脱落酸, 抗寒力, 脯氨酸

Abstract: Effect of cold acclimation and ABA on cold hardiness resistance, contents of proline in tea plants was studied. The results showed that through cold acclimation and ABA treatment, the cold hardiness of tea plants was changed greatly during the process of acclimation to deacclimation, showing a trend from low to high then to low again. And ascertain 7℃and 9℃are the crucial temperature to finish acclimation or start declimation respectively.On the other hand, it can be concluded that the rising degree of cold hardiness resistance was lower during artificial or ABA treatment than that of natural cold acclimation. At the same time, changes of Proline content weren’t regular,the relationship between proline content and cold hardiness resistance was insignificant. It implied that changes of proline content during cold acclimation were an integrative response in tea plant.

Key words: Tea science, Tea plant[Camellia sinensis(L.) O. Kuntze], Cold acclimation, ABA, Cold hardiness resistance, Proline

中图分类号:

杨亚军, 郑雷英, 王新超. 冷驯化和ABA对茶树抗寒力及其体内脯氨酸含量的影响[J]. 茶叶科学, 2004, 24(3): 177-182. doi: 10.13305/j.cnki.jts.2004.03.006.

YANG Ya-jun, ZHEN Lei-ying, WANG Xin-chao. Effect of Cold Acclimation and ABA on Cold Hardiness, Contents of Proline in Tea Plants[J]. Journal of Tea Science, 2004, 24(3): 177-182. doi: 10.13305/j.cnki.jts.2004.03.006.

参考文献 22

[1]	Miren A and Corcuera. Cold acclimation in plants[J] . Phytochemistry, 1991,30(10):3177~3184.
[2]	Gilmour S J, Hajela R K,Thomashow M F.Cold acclimation in Arabidopsis thaliana[J]. Plant Physiol.,1988, 87: 745~750.
[3]	Gray G R, Chauvin L P, Sarhan F, et al. Cold acclimation and freezing tolerance[J]. Plant Physiol., 1997,114: 467~474.
[4]	邵继荣,刘永胜,周仕春,等. 冷锻炼对提高水稻幼苗抗寒性及其细胞器膜结构稳定性的影响[J]. 作物学报,1999,25(5):569~573.
[5]	Xin Z, Browse J.Cold comfort farm: the acclimation of plants to freezing temperature[J]. Plant cell and Envrionment, 2000, 23: 893~902.
[6]	杨盛昌,谢潮添,张平,等. 冷锻炼对低温胁迫下夏威夷椰子膜脂过氧化及保护酶活性的影响[J]. 植物资源与环境学报,2002,11(4):25~28.
[7]	林定波,刘祖祺. 冷驯化和ABA对柑桔膜稳定性的影响及膜特异蛋白的诱导[J]. 南京农业大学学报,1994,17(1):1~5.
[8]	吴楚,王政权. 脱落酸及其类似物与植物抗寒性之间的关系[J].植物生理学通讯,2000,36(6):562~567.
[9]	黄华涛,刘祖生,庄晚芳. 茶树抗寒生理的研究–酶和细胞膜透性与茶树抗寒性[J]. 茶叶科学,1986,6(1):41~48.
[10]	王新超,杨亚军. 茶树抗性育种研究现状[J]. 茶叶科学,2003,23(4):94~98.
[11]	陈翠莲,马平福. 抗冷性不同的小麦、水稻品种脯氨酸含量的比较试验[J]. 华中农业大学学报,1989,8(2):176~179.
[12]	高志强,张国红,张爱芝,等. 不同小麦品种对低温的生理反应研究[J]. 山西农业大学学报,2002,22(2):109~112.
[13]	马建灿,张玉法,杨天柱. 低温胁迫对喜树幼苗SOD活性、MDA和脯氨酸含量的影响[J]. 林业科学研究,2002,15(2):197~202.
[14]	李建设,耿广东,程智慧. 低温胁迫对茄子幼苗抗寒性生理生化指标的影响[J]. 西北农林科技大学学报(自然科学版),2003,3(11):90~93.
[15]	李卫,孙中海,章文才,等. 柑橘抗寒育种早期鉴定的一种指标[J]. 植物学报,1998,40(9):827~830.
[16]	朱广廉,邓兴旺,左卫能. 植物体内游离脯氨酸的测定[J]. 植物生理学通讯,1983,(1):35~37.
[17]	潘瑞炽主编. 植物生理学(第四版)[M]. 北京:高等教育出版社,2001.281~282.
[18]	中国农业科学院茶叶研究所主编. 中国茶树栽培学[M]. 上海:上海科学技术出版社,1986.94~95.
[19]	苏维埃. 植物对温度逆境的适应[A]. 见:余叔文,汤章城主编.植物生理与分子生物学(第二版)[M].北京:科学出版社,1998.721~738.
[20]	Delauney A J.Verma DPS Proline biosynthesis and osmoregulation in plants[J]. Plant J, 1993, 4: 215~223.
[21]	Xin Z and Li P H. Abscisic acid - induced chilling tolerance in maize suspension - cultured cells[J]. Plant Physiol, 1992, 99: 707~7111.
[22]	Xin Z and Li P H. Alteration of gene expression associated with abscisic acid - induced chilling tolerance in maize suspension-cultured cells[J]. Plant Physiol., 1993, 101: 277~284.