外源5-ALA对干旱胁迫下茶树叶绿素合成和荧光特性及关键酶基因表达的影响

doi:10.13305/j.cnki.jts.2022.02.010

茶叶科学 ›› 2022, Vol. 42 ›› Issue (2): 187-199.doi: 10.13305/j.cnki.jts.2022.02.010

外源5-ALA对干旱胁迫下茶树叶绿素合成和荧光特性及关键酶基因表达的影响

杨妮¹, 李逸民¹, 李静文¹, 滕瑞敏¹, 陈益¹, 王雅慧², 庄静^1,*

1.南京农业大学园艺学院,茶叶科学研究所,农业部华东地区园艺作物生物学与种质创制重点实验室,江苏南京 210095;
2.南京农业大学,作物遗传与种质创新国家重点实验室,江苏南京 210095

收稿日期:2021-11-11 修回日期:2021-12-23 出版日期:2022-04-15 发布日期:2022-04-15
通讯作者: * zhuangjing@njau.edu.cn
作者简介:杨妮,女,博士研究生,主要从事茶树分子生物学研究,2021204040@stu.njau.edu.cn。
基金资助:
江苏省农业科技自主创新资金项目（CX(20)3114）、江苏高校优势学科建设项目（PAPD）

Effects of Exogenous 5-ALA on the Chlorophyll Synthesis and Fluorescence Characteristics and Gene Expression of Key Enzymes in Tea Plants under Drought Stress

YANG Ni¹, LI Yimin¹, Li Jingwen¹, TENG Ruimin¹, CHEN Yi¹, WANG Yahui², ZHUANG Jing^1,*

1. Ministry of Agriculture Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in East China, Tea Research Institute, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China;
2. State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China

Received:2021-11-11 Revised:2021-12-23 Online:2022-04-15 Published:2022-04-15

摘要/Abstract

摘要： 为探究外源5-氨基乙酰丙酸（5-ALA）在茶树幼苗响应干旱胁迫时对茶树叶绿素合成和荧光特性的调控机理,以舒茶早为试验材料,PEG-6000模拟干旱胁迫环境,喷施5-ALA进行处理,检测茶树幼苗叶片的叶绿素a、叶绿素b和总叶绿素含量,进一步测定叶片叶绿素荧光参数及关键酶基因的表达。结果显示,外源5-ALA显著提高干旱胁迫下茶树叶片叶绿素a、叶绿素b、总叶绿素的含量,缓解了最大荧光（Fm）、PSⅡ实际光化学效率[Y(Ⅱ)]、PSⅡ最大光化学量子产量（Fv/Fm）、光化学猝灭系数（qP）、PSⅡ潜在活性（Fv/Fo）、PSⅡ反应中心光合电子传递效率（Electron transfer rate,ETR）的下降,同时导致初始荧光（Fo）、非光化学猝灭系数（qN）升高。外源5-ALA能诱导干旱胁迫下茶树编码叶绿素合成（CsHEMA1、CsHEME1、CsLIN2）以及碳同化（CsSBPase、CsTK）相关酶基因的上调表达。研究表明,叶面喷施外源5-ALA能有效缓解干旱胁迫对茶树叶片叶绿素的降解及对PSⅡ反应中心的损伤,维持茶树叶片较高的光合活性,提高其光保护能力。

关键词: 茶树, 5-氨基乙酰丙酸, 干旱胁迫, 叶绿素荧光参数, 基因表达

Abstract: In order to study the regulation mechanism of exogenous 5-aminolevulinic acid (5-ALA) on the chlorophyll synthesis and fluorescence characteristics of tea plants in response to drought stress, tea cultivar 'Shuchazao' was used as the experimental material in this study. Under PEG-6000 simulated drought stress environment and 5-ALA spraying pretreatment, the contents of chlorophyll a, chlorophyll b and total chlorophyll in tea seedling leaves were detected, and the chlorophyll fluorescence parameters and the expressions of key enzyme genes in tea leaves were further determined. The results show that exogenous 5-ALA significantly increased the contents of chlorophyll a, chlorophyll b and total chlorophyll in tea leaves under drought stress. Exogenous 5-ALA alleviated the decreases of maximum fluorescence (Fm), actual photochemical efficiency of PSⅡ [Y(Ⅱ)], maximum photochemical quantum yield of PSⅡ (Fv/Fm), photochemical quenching coefficient (qP), potential activity of PSⅡ(Fv/Fo), photosynthetic electron transfer rate (ETR) of PSⅡ reaction center, and the increases of initial fluorescence (Fo) and nonphotochemical quenching coefficient (qN). At the same time, exogenous 5-ALA induced the expressions of chlorophyll synthesis (CsHEMA1, CsHEME1, CsLIN2) and carbon assimilation-related enzymes (CsSBPase, CsTK) in tea plant under drought stress. The study demonstrates that spraying exogenous 5-ALA can effectively alleviate the degradation of chlorophyll and damage to PSⅡ reaction center of tea leaves under drought stress, maintain the higher photosynthetic activity, and improve the photoprotection ability of tea leaves.

Key words: tea plant, 5-aminolevulinic acid, drought stress, chlorophyll fluorescence parameters, gene expression

中图分类号:

S571.1

杨妮, 李逸民, 李静文, 滕瑞敏, 陈益, 王雅慧, 庄静. 外源5-ALA对干旱胁迫下茶树叶绿素合成和荧光特性及关键酶基因表达的影响[J]. 茶叶科学, 2022, 42(2): 187-199. doi: 10.13305/j.cnki.jts.2022.02.010.

YANG Ni, LI Yimin, Li Jingwen, TENG Ruimin, CHEN Yi, WANG Yahui, ZHUANG Jing. Effects of Exogenous 5-ALA on the Chlorophyll Synthesis and Fluorescence Characteristics and Gene Expression of Key Enzymes in Tea Plants under Drought Stress[J]. Journal of Tea Science, 2022, 42(2): 187-199. doi: 10.13305/j.cnki.jts.2022.02.010.

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外源5-ALA对干旱胁迫下茶树叶绿素合成和荧光特性及关键酶基因表达的影响

Effects of Exogenous 5-ALA on the Chlorophyll Synthesis and Fluorescence Characteristics and Gene Expression of Key Enzymes in Tea Plants under Drought Stress

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