茶树白化变异研究进展

卢翠; 沈程文

doi:10.13305/j.cnki.jts.2016.05.001

PDF(730 KB)

茶叶科学 ›› 2016, Vol. 36 ›› Issue (5) : 445-451. DOI: 10.13305/j.cnki.jts.2016.05.001

茶树白化变异研究进展

卢翠, 沈程文^*

作者信息 +

Research Progress of Albino Tea Plant Camellia sinensis (L.) O. Kuntze）

LU Cui, SHEN Chengwen^*

Author information +

文章历史 +

摘要

白化现象在植物界普遍存在,茶叶作为一种叶用植物,白化变异植株中关键化学成分如叶绿素、类胡萝卜素、儿茶素类、氨基酸类、黄酮类等物质含量发生了显著变化,这些变化对茶树生长发育、抗逆性、茶叶制作工艺、口感等有重要影响。叶色变异作为一种突变性状对于茶树新品种选育有着重要的应用价值,对研究茶树光合作用机制、叶色调控机理、叶片发育调控等也有重要理论价值。本文综述了国内近几年关于茶树白化突变体化学成分叶绿素、类胡萝卜素、氨基酸、儿茶素的研究及茶树白化突变分子机制的研究进展,旨在为茶树白化突变体分子机制的深入研究提供参考。

Abstract

Leaf albino is a common phenomenon in the plant kingdom. As a kind of leaf-used plant, the key chemical ingredients in albino tea cultivars such as chlorophyll, carotenoids, catechins, amino acids, flavonoids are significantly changed as compared with normal tea cultivars, which largely affect the growth, stress resistances, production process and taste of tea. Leaf color mutation, as a special mutation, has important application value for tea breeding. It is also useful for our understanding of the photosynthesis system, the regulation mechanism of leaf color and the regulation of leaf development in tea plants. This article reviewed the research progress on the chemical compositions such as chlorophyll, carotenoids, amino acids and the mechanism of the mutation in the albino tea cultivars, which aimed to provide a foundation for further study of the molecular mechanism in albino tea cultivars.

导出引用

卢翠, 沈程文. 茶树白化变异研究进展[J]. 茶叶科学. 2016, 36(5): 445-451 https://doi.org/10.13305/j.cnki.jts.2016.05.001

LU Cui, SHEN Chengwen. Research Progress of Albino Tea Plant Camellia sinensis (L.) O. Kuntze）[J]. Journal of Tea Science. 2016, 36(5): 445-451 https://doi.org/10.13305/j.cnki.jts.2016.05.001

中图分类号： S571.1 S432.1

参考文献

[1] 何冰, 刘玲珑, 张文伟, 等. 植物叶色突变体[J]. 植物生理学通讯, 2006, 42(1): 1-9.
[2] 李家贤. 浅述华南茶区茶树品种选育的途径[J]. 广东茶叶科技, 1983(4): 6-10, 45.
[3] 王开荣. 白化茶种质资源综合性状研究[D]. 杭州: 浙江大学, 2006: 14-15, 60-62.
[4] Yuan L, Xiong L G, Deng T T, et al.Comparative profiling of gene expression in Camellia sinensis L. Cultivar AnJiBaiCha leaves during periodic albinism[J]. Gene, 2015, 561(1): 23-29.
[5] 王开荣. 白茶种质资源利用刍议[J]. 茶叶, 2003, 29(4): 217-219.
[6] 王开荣, 李明, 梁月荣, 等. 茶树新品种黄金芽选育研究[J]. 中国茶叶, 2008, 30(4): 21-23.
[7] 李明, 张龙杰, 王开荣, 等. 光照敏感型白化茶新品种“黄金芽”白化特性研究[J]. 茶叶, 2008, 34(2): 98-102.
[8] 韩震, 王开荣, 邓隆, 等. 白化茶树新品系——黄金斑[J]. 茶叶, 2013, 39(3): 125-126.
[9] 姚江, 胡建程, 谢丰镐. 茶叶中叶绿素的研究[J]. 浙江农业大学学报, 1990, 16(4): 87-92.
[10] Demmig A B, Adams W W.The role of xanthophyll cycle carotenoids in the protection of photo synthesis[J]. Trends Plant Sci, 1996,1(1): 21-26.
[11] Walter M H, Floss D S, Strack D.Apocarotenoids: Hormones, mycorrhizal metabolites and aroma volatiles[J]. Planta, 2010, 232(1): 1-17.
[12] Feng L, Gao M J, Hou R Y, et al.Determination of quality constituents in the young leaves of albino tea cultivars[J]. Food Chemistry, 2014, 155(11): 98-104.
[13] Havaux M.Carotenoids as membrane stabilizers in chloro Plasts[J]. Trends in Plant Scienee, 1998, 3(4): 147-151.
[14] 李靓. 茶氨酸保健功效研究及其保健食品开发[D]. 北京: 中国农业科学院, 2009: 2-6.
[15] 杜颖颖. 新梢白化茶树品种白化机理研究[D]. 杭州: 浙江大学, 2009: 35-37.
[16] 成浩, 李素芳, 陈明, 等. 安吉白茶特异性状的生理生化本质[J]. 茶叶科学, 1999, 19(2): 87-92.
[17] 李素芳, 成浩, 虞富莲, 等. 安吉白茶阶段性返白过程中氨基酸的变化[J]. 茶叶科学, 1996, 16(2): 75-76.
[18] 李素芳, 陈明, 虞富莲, 等. 茶树阶段性返白现象的研究—RuBP羧化酶与蛋白酶的变化[J]. 中国农业科学, 1999, 32(3): 35-40.
[19] Yuan L, Xiong L G, Deng T T, et al.Comparative profiling of gene expression in Camellia sinensis L. cultivar Anjibaicha leaves during periodic albinism[J]. Gene, 2015, 561(1): 23-29.
[20] 夏涛, 高丽萍. 类黄酮及茶儿茶素生物合成途径及其调控研究进展[J]. 中国农业科学, 2009, 42(8): 2899-2908.
[21] Kang Wei, Li-Yuan Wang, Jian Zhou, et al.Comparison of catechins and purine alkaloids in albino and normal green tea cultivars (Camellia sinensis L.) by HPLC[J]. Food Chem, 2012, 130(3): 720-724.
[22] Xiong L G, Li J, Li Y H, et al.Dynamic changes in catechin levels and catechin biosynthesis-related gene expression in albino tea plants (Camellia sinensis L.)[J]. Plant Physiology and Biochemistry, 2013, 71(71C): 132-143.
[23] 朱明库, 胡宗利, 周爽, 等. 植物叶色白化研究进展[J]. 生命科学, 2012, 24(3): 255-261.
[24] Ma C L, Chen L, Wang X C, et al.Differential expression analysis of different albescent stages of ‘Anjibaicha’ (Camellia sinensis (L.) O. Kuntze) using cDNA microarray[J]. Scientia Horticulturae, 2012, 148(4): 246-254.
[25] Chun-Fang Li, Ming-Zhe Yao, Chun-Lei Ma, et al.Differential Metabolic Profiles during the Albescent Stages of ‘Anjibaicha’ (Camellia sinensis)[J]. PLoS One, 2015, 10(10): e0139996.
[26] Guy C L.Cold acclimation and freezing stress tolerance: role of protein metabolizing[J]. Annual Review of Plant Biology, 1990, 41(1): 187-223.
[27] 李素芳, 陈树尧, 成浩. 茶树阶段性返白现象的研究——叶绿体超微结构的变化[J]. 茶叶科学, 1995, 15(1): 23-26.
[28] 邓婷婷. 不同温度下安吉白茶差异表达基因的分离、鉴定及CsPDS3基因的功能研究[D]. 长沙: 湖南农业大学, 2013: 33-37.
[29] 马娇. 水稻叶缘白化突变体mal的遗传分析与基因定位[D]. 重庆: 西南大学, 2014: 5-7.
[30] 李智. 不同环境因子调控茶树紫色芽叶形成的分子机制研究[D]. 泰安: 山东农业大学, 2014: 13-15.
[31] Li N N, Yang Y P, Ye J H, et al.Effects of sunlight on gene expression and chemical composition of light-sensitive albino tea plant[J]. Plant Growth Regulation, 2016, 78(2): 253-262
[32] 金蓉, 朱长青, 徐昌杰. 1-脱氧木酮糖-5-磷酸合成酶(DXS)及其编码基因[J]. 细胞生物学杂志, 2007, 29(5): 706-712.
[33] Lois LM, Campos N, Putra S R, et al.Cloning and characterization of a gene from Escherichia coli encoding a transketolase-like enzyme that catalyzes the synthesis of D-1-deoxyxylulose 5-phosphate, a common precursor for isoprenoid, thiamin, and pyridoxol biosynthesis[J]. Proceedings of the National Academy of Sciences of the United States of America, 1998, 95(5): 2105-2110.
[34] K R Wang, N N Li, Y Y Du, et al.Effect of sunlight shielding on leaf structure and amino acids concentration of light sensitive albino tea plant[J]. African Journal of Biotechnology, 2013, 12(36): 5535-5539.
[35] 张龙杰, 王开荣, 鲁松才, 等. 低温敏感型白化茶栽培与加工技术[J]. 中国茶叶, 2008, 30(10): 14-15.
[36] 陆文渊, 钱文春, 赖建红, 等. 安吉白茶产业的现状及发展对策[J]. 茶叶科学技术, 2012(1): 25-27.
[37] Wang K R, Du Y Y, Shao S H, et al.Development of specific RAPD markers for identifying albino tea cultivars ‘Qiannianxue’ and ‘Xiaoxueya’[J]. African Journal of Biotechnology, 2010, 9(4): 434-437.
[38] Chen Y, Deng J, Wang Y X, et al.Study on discrimination of white tea and albino tea based on near infrared spectroscopy and chemometrics[J]. Sci Food Agric, 2014, 94(5): 1026-1033.
[39] 陈春梅, 马春雷, 马建强, 等. 茶树cpDNA测序及基于cpDNA序列的山茶属植物亲缘关系研究[J]. 茶叶科学, 2014, 34(4): 371-380.
[40] 叶晓倩, 赵忠辉, 朱全武, 等. 茶树“龙井43”叶绿体基因组测序及其系统进化(英文)[J]. 浙江大学学报: 农业与生命科学版, 2014, 40(4): 404-412.
[41] 李娜娜. 新梢白化茶树生理生化特征及白化分子机理研究[D]. 杭州: 浙江大学, 2015: 12-13.