保留指数在茶叶挥发物鉴定中的应用及保留指数库的建立

林杰; 陈莹; 施元旭; 王校常

doi:10.13305/j.cnki.jts.2014.03.008

茶叶科学 >

2014 , Vol. 34 >Issue 3: 261 - 270

DOI: https://doi.org/10.13305/j.cnki.jts.2014.03.008

保留指数在茶叶挥发物鉴定中的应用及保留指数库的建立

林杰 ,
陈莹 ,
施元旭 ,
王校常

展开

1. 浙江大学茶学系,浙江杭州 310058;
2. 浙江农林大学农业与食品科学学院,浙江省农产品品质改良技术研究重点实验室,浙江杭州 311300;
3. 杭州市质量技术监督检测院,浙江杭州 310019

林杰（1985— ）,男,浙江温州人,助教,博士,主要从事茶叶品质化学研究。

收稿日期: 2013-06-18

修回日期: 2013-09-02

网络出版日期: 2019-09-03

收起

Application of Retention Index on Volatile Compound Identification of Tea and Development of Retention Index Database

LIN Jie ,
CHEN Ying ,
SHI Yuanxu ,
WANG Xiaochang

Expand

1. Institute of Tea Science, Zhejiang University, Hangzhou 310058, China;
2. The Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province, Zhejiang A&F University, Hangzhou 311300, China;
3. Hangzhou Institute of Calibration and Testing for Quality and Technical Supervision, Hangzhou 310019, China

Received date: 2013-06-18

Revised date: 2013-09-02

Online published: 2019-09-03

Fold

摘要

采用气相色谱/质谱联用（GC-MS）技术,分别应用正构烷烃（n-alkanes）混标和分析物中的正构烷烃对茶叶保留指数（RI）进行了测定,得到的保留时间相对碳数的线性相关系数（R²）均超过了0.99,证实了两种保留指数测定方法的可靠性。在谱库检索的基础上结合保留指数法,龙井茶香气成分的鉴定率从46.67%提高到74.67%,大多数物质可得到完全鉴定。同时,保留指数能对质谱匹配过程中存在的同分异构体进行有效的鉴定,提高物质定性的准确性,使物质鉴定效率大大提高。归纳相关文献数据,构建了HP-innowax色谱柱的保留指数库,提高了利用HP-innowax色谱柱进行挥发物分析的效率。

关键词： 保留指数; 茶叶; 挥发物; HS-SPME; 保留指数库

本文引用格式

林杰 , 陈莹 , 施元旭 , 王校常 . 保留指数在茶叶挥发物鉴定中的应用及保留指数库的建立[J]. 茶叶科学, 2014 , 34(3) : 261 -270 . DOI: 10.13305/j.cnki.jts.2014.03.008

Abstract

Standard mixture of n-alkanes and n-alkanes in samples were applied respectively to determine the retention index (RI) of tea volatiles. The linear correlation coefficients of retention time with carbon number for the two methods were both above 0.99, indicating quite good reliability for calculating RI. With the applying of RI, the correct identification rate of the volatiles increased from 46.67% to 74.67%. Meanwhile, RI enabled effective identification of the isomers contained in tea volatiles, and greatly increased the identification accuracy. Data in relevant literatures were summarized to construct a RI database for HP-innowax column. The RI database could also improve the efficiency of compound identification using HP-innowax column.

Key words： retention index; tea; volatiles; HS-SPME; retention index database

参考文献

[1] 梁晟, 李雅文, 赵晨曦, 等. GC-MS结合保留指数对中药挥发油的定性[J]. 分析测试学报, 2008, 27(1): 84-87.
[2] 苏越, 姬厚伟, 苏式兵, 等. GC/MS中保留指数和保留温度回归分析应用于单萜烯结构确认[J]. 分析测试技术与仪器, 2008, 14(3): 131-135.
[3] 苏越, 刘素红, 王呈仲, 等. 谱图相似度分析结合保留指数对单萜烯同分异构体的GC-MS定性分析[J]. 分析测试学报, 2009, 28(5): 525-528.
[4] Lucero M, Estell R, Tellez M, et al. A retention index calculator simplifies identification of plant volatile organic compounds[J]. Phytochemical Analysis, 2009, 20: 378-384.
[5] De Castro O, Senatore F, Rigano D, et al. Composition of the essential oil of Petagnaea gussonei (Sprengel) Rauschert, a relict species from Sicily (Southern Italy)[J]. Flavour and Fragrance Journal, 2008, 23: 172-177.
[6] Zhao CX, Liang YZ, Fang HZ, et al. Temperature-programmed retention indices for gas chromatography-mass spectroscopy analysis of plant essential oils[J]. Journal of Chromatography A, 2005, 1096: 76-85.
[7] Grujic-Jovanovic S, Skaltsa HD, Marin P, et al. Composition and antibacterial activity of the essential oil of six Stachys species from Serbia[J]. Flavour and Fragrance Journal, 2004, 19: 139-144.
[8] Chung, MS.Volatile compounds of the Hallabong (Citrus kiyomi × Citrus ponkan) blossom[J]. Food Science and Biotechnology, 2013, 21: 285-290.
[9] L, R, Jiang ZT. Chemical composition of the essential oil of Cuminum cyminum L. from China[J]. Flavour and Fragrance Journal, 2004, 19: 311-313.
[10] Yang DS, Shewfelt RL, Lee KS, et al. Comparison of odor-active compounds from xix distinctly different rice flavor types[J]. Journal of Agricultural and Food Chemistry, 2008, 56: 2780-2787.
[11] Pino JA, Quijano C, CE. Volatile constituents of camu-camu (Myrciaria dubia (HBK) McVaugh) leaves[J]. Journal of Essential Oil Research, 2012, 20: 205-207.
[12] Pino J, Sauri-Duch E, Marbot R.Changes in volatile compounds of Habanero chile pepper (Capsicum chinense Jack. cv. Habanero) at two ripening stages[J]. Food Chemistry, 2006, 94: 394-398.
[13] Heravi MJ, Sereshti H.Determination of essential oil components of Artemisia haussknechtii Boiss. using simultaneous hydrodistillation-static headspace liquid phase microextraction-gas chromatography mass spectrometry[J]. Journal of Chromatography A, 2007, 1160: 81-89.
[14] Shang C, Hu Y, Deng C, et al. Rapid determination of volatile constituents of Michelia alba ?owers by gas chromatography-mass spectrometry with solid-phase microextraction[J]. Journal of Chromatography A, 2002, 942: 283-288.
[15] Habibi Z, Masoudi S, Rustaiyan A.Essential oil of Nepeta makuensis jamzad et mozaffarian from Iran[J]. Journal of Essential Oil Research, 2004, 16: 214-215.
[16] Senatore F, Arnold NA, Piozzi F, et al. Chemical composition of the essential oil of Salvia microstegia Boiss. et Balansa growing wild in Lebanon[J]. Journal of Chromatography A, 2006, 1108: 276-278.
[17] Akhgar MR, Rustaiyan A, Masoudi S, et al. Essential oils of Ferula microcolea (Boiss.) Boiss. and Ferula hirtella Boiss. from Iran[J]. Journal of Essential Oil Research, 2005, 17: 237-238.
[18] Mihailovic V, Vukovic N, Niciforovic N, et al. Studies on the antimicrobial activity and chemical composition of the essential oils and alcoholic extracts of Gentiana asclepiadea L[J]. Journal of Medicinal Plants Research, 2011, 5: 1164-1174.
[19] Maggi F, Bilek T, Cristalli G, et al. Comparison of the characterization of the fruit-like aroma of Teucrium ?avum L. subsp ?avum by hydrodistillation and solid- phasemicro-extraction[J]. Journal of the Science of Food and Agriculture, 2009, 89: 2505-2518.
[20] Kim TH, Thuy NT, Shin JH, et al. Aroma-active compounds of miniature beefsteakplant (Mosla dianthera Maxim.)[J]. Journal of Agricultural and Food Chemistry, 2000, 48: 2877-2881.
[21] Salido S, Altarejos J, Nogueras M, et al. Chemical studies of essential oils of Juniperus oxycedrus ssp. badia[J]. Journal of Ethnopharmacology, 2002, 81: 129-134.
[22] Haghi G, Ghasian F, Safaei-Ghomi J.Determination of the essential oil from root and aerial parts of Artemisia dracunculus L. cultivated in central Iran[J]. Journal of Essential Oil Research, 2010, 22: 294-296.
[23] do Nascimento ME, Zoghbi MGB, Pinto JEBP, et al. Chemical variability of the volatiles of Copaifera langsdorfﬁi growing wild in the Southeastern part of Brazil[J]. Biochemical Systematics and Ecology, 2012, 43: 1-6.
[24] Hazzit M, Baaliouamer M, Faleiro ML, et al. Composition of the essential oils of Thymus and Origanum species from Algeria and their antioxidant and antimicrobial activities[J]. Journal of Agricultural and Food Chemistry, 2006, 54: 6314-6321.
[25] De Martino L, Roscigno G, Mancini E, et al. Chemical composition and antigerminative activity of the essential oils from five Salvia species[J]. Molecules, 2010, 15: 735-746.
[26] Zhang Y, Gao B, Zhang M, et al. Headspace solid-phase microextraction-gas chromatography-mass spectrometry analysis of the volatile components of longan (Dimocarpus longan Lour.)[J]. European Food Research and Technology, 2009, 229: 457-465.
[27] Liolios C, Laouer H, Boulaacheb N, et al. Chemical composition and antimicrobial activity of the essential oil of Algerian Phlomis bovei De Noé subsp. Bovei[J]. Molecules, 2007, 12: 772-781.
[28] Kenig F, Simons DH, Crich D, et al. Structure and distribution of branched aliphatic alkanes with quaternary carbon atoms in Cenomanian and Turonian black shales of Pasquia Hills (Saskatchewan, Canada)[J]. Organic Geochemistry, 2005, 36: 117-138.
[29] Senatore F, Arnold NA, Piozzi F.Chemical composition of the essential oil of Salvia multicaulis Vahl. var. simplicifolia Boiss. growing wild in Lebanon[J]. Journal of Chromatography A, 2004, 1052: 237-240.
[30] Vichi S, Pizzale L, Conte LS, et al. Simultaneous determination of volatile and semi-volatile aromatic hydrocarbons in virgin olive oil by headspace solid-phase microextraction coupled to gas chromatography/mass spectrometry[J]. Journal of Chromatography A, 2005, 1090: 146-154.
[31] Adams RP.Identification of essential oil components by gas chromatography/quadrupole mass spectroscopy[M]. Allured: Carol Stream, IL, 2001.
[32] Adams RP.Identification of essential oil components by gas chromatography/mass spectrometry[M]. Allured: Carol Stream, IL, 2007.

Options

文章导航

模态框（Modal）标题

摘要

本文引用格式

Abstract

参考文献