利用cDNA-AFLP技术研究了紫娟茶树幼嫩叶和成熟叶之间的基因表达差异,获得在幼嫩叶中高表达的差异片段TDF（Transcript derived fragment）,再利用RACE方法首次从茶树中克隆了APETALA2（AP2）转录因子基因的全长cDNA,其开放阅读框编码518个氨基酸,含有2个AP2结构域,与多种植物APETALA2蛋白具有高度同源性,属于AP2亚族,命名为CsAP2。茶树APETALA2（AP2）转录因子基因的克隆为进一步研究该基因在茶树花发育调控中的作用奠定了基础。
The cDNA-AFLP was applied to identify genes expressed differentially between young and mature leaves of tea plant (Camellia sinensis var. assamica, cultivar Zijuan). A cDNA fragment, TDF encoding a APETALA2 was isolated, and containing a complete coding sequence cDNA was cloned by RACE, which encodes a polypeptide of 518 amino acids including two conserved AP2 domains, named CsAP2, sequence alignment showed that CsAP2 protein shared high identity with other plants. APETALA2 gene cloning provided foundation for studying flower development in tea plant.
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