采用SSH技术分析了VA菌根处理后福鼎大白茶根系基因差异表达情况,获得了差异序列,序列比对显示,在上调表达序列中包含了3-羟基-3-甲戊二酸单酰辅酶A还原酶（3-hydroxy-3-methylglutaryl coenzyme A reductase,HMGR）。采用RACE技术获得了HGMR基因全长序列,HGMR基因长2 420 bp,具有1 773 bp开放阅读框（163rd~1935th）,编码590个氨基酸。分子生物信息学分析表明,HGMR蛋白分子量约63.5 kD,等电点为6.8,定位于线粒体膜或者内质网膜。研究还显示,HGMR在茶树叶片中表达强度存在明显的品种差异,同时,HGMR对生物性和非生物性胁迫均有明显响应。
By using SSH, the differences in gene expression of root system from Fuding Dabai tea infected by VA mycorrhiza were analyzed and diversity sequences were obtained. The sequence alignment showed that the mentioned sequence contain 3-hydroxy-3-methylglutaryl coenzyme A reductase named HMGR. HGMR full-length sequence was obtained by using RACE. The length of HGMR gene is 2 420 bp, with 1 773 bp ORF (163rd-1935th), and the sequence encoded 590 amino acids. Bioinformatics indicated that the HGMR protein’s molecular weight is about 63.5 kD, IEP is 6.8, which located in mitochondria or endoplasmic reticulum membrane. The study also showed expression degree of HGMR is distinct in different cultivars, while it responded obviously to biological and non-biological stress.
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