以壳聚糖作为载体,戊二醛为交联剂制备固定化纤维素酶,对夏季绿茶进行酶解。通过正交实验得到优化工艺：在1.0g茶叶中,以茶水比1︰20,加入1.0g固定化纤维素酶,55℃下恒温浸提50min。与传统高温水提法所得茶汤相比,以此工艺条件浸提所得茶汤中茶多酚、氨基酸、咖啡碱含量有所提高,酚氨比有所下降,连续提取7批次茶叶,固定化纤维素酶的相对酶活力仍保持80%以上。
Immobilization of cellulase on carrier of chitosan beeds crosslinked by glutaraldehyde were studied, and applied the immobilized cellulase to the hydrolysis of the summer green tea. Through the orthogonal test, the optimal extract technology has been investigated: adding 1.0g immobilized cellulose enzyme in 1.0g green tea, the ratio of tea and water is 1∶20,and extracted 50min under 55℃. Compared to the traditional tea extraction method by hot water, the contents of tea polyphenols, amino acid and caffeine increased by using this technology, and the ratio of polyphenol to ammo acid was decreased. The immobilized cellulase still maintain a high activity, the relative activity of enzyme was more than 80%, after 7 times extraction.
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