以茶树叶片为材料,结合同源克隆方法和RACE技术,克隆了1条UFGT基因,命名为CsUFGT。该基因cDNA全长为1526bp,ORF长1380bp,编码459个氨基酸,推测等电点5.96,推测分子量为49.486kDa。该基因编码的蛋白质与葡萄UFGT（P51094.2）的一致性为59%,相似性为75%,其C-端含有植物UGT家族成员特有PSPG基序。荧光实时定量PCR分析表明,该基因在茶树根茎叶中均表达,在第4叶表达量最高,根和茎中表达量较低。
A glucosyltransferase gene UDP-flavonoid 3-O-glucosyl transferase was isolated from tea plant [Camellia sinensis (L.) O. Kuntze] and named CsUFGT. CsUFGT has 1526bp full length with open reading frame of 1380bp which encodes 459 amino acids. The corresponding protein CsUFGT, with predicted molecular mass 49.486kDa and predicted isoelectric point 5.96, shares 59% identity and 75% similarity with UFGT(P51094.2) in Vitis vinifer. CsUFGT includes a PSPG signal motif of typical plant glucosyltransferase. qRT-PCR analysis showed that the gene expressed in all tissues of tea plant [Camellia sinensis (L.) O. Kuntze], and had high expression in the fourth leaf and low expression level in root and stem.
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