利用ISSR技术对四川、重庆等地12份重要的野生大茶树资源的遗传多态性进行了分析。从60个随机引物中筛选出7个多态性引物,共扩增出146条DNA带,其中139条为多态性带,占95.2％,平均每个引物扩增的DNA带数为21条。按照相同迁移位上有扩增带记为1、无带为0的方法构建ISSR表型数据矩阵,供试品种的基因（H）和shannon信息系数分别为0.20和0.34。对12株野生大茶树进行UPGMA聚类分析,品种间的相似系数介于0.37~0.71,平均为0.51。并在此基础上建立了聚类分析树状图,将其12个品种划分为3大聚类组。
The ISSR markers were used to analysis the genetic diversity of 12 important wild tea plants in the areas of Sichuan and Chongqing. Seven primers were screened from 60 random primers, and a total of 146 DNA bands were amplified, among which 139(95.2%) were polymorphic. The average number of DNA bands was 21. ISSR data matrix was built according to this method which is to record 1 when there is an amplified band, and 0 when there is not. Nei' s gene diversity (H) and Shannon's information index (I) were 0.20 and 0.34, respectively. Through the UPGAM cluster analysis of 12 wild tea plants, the genetic similarity among all the tested wild teas ranged from 0.37 to 0.71, averaging 0.51. Based on it, a DNA molecular dendrogram was established. The dendrogram divided the 12 wild teas into three groups.
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