普洱茶水提物依次经氯仿、乙酸乙酯、正丁醇萃取后剩余的水层色素-茶褐素上羟丙基葡聚糖凝胶（sephadex LH-20）柱,以丙酮-水溶液进行洗脱,按照色素条带分别收集得到级分并初步研究它们的理化与光谱性质。结果表明,以40%丙酮-水溶液为洗脱剂,流速为0.05BV/h时,茶褐素的分级效果最好,柱中呈现6条明显的条带,它们的色差参数值、还原力、pH值、常规成分含量、紫外-可见光谱扫描、红外光谱扫描及稳定性等存在差异。该研究进一步表征了普洱茶茶褐素组成与性质的复杂性,同时为深入研究茶褐素的生物活性与化学成分的本质奠定了基础。
The water extract of pu-erh tea was extracted with chloroform, ethyl acetate, n-butanol in turn. The remaining water pigment-theabrownin obtained was installed in hydroxypropyl glucan gel (sephadex LH-20) column with acetone-aqueous solution for elution and the fractions of theabrownin were collected respectively and their physico-chemical and spectral properties were studied preliminarily. The results indicated that the 40% acetone-aqueous solution was the optimum eluent and flow was 0.05BV/h and classification of theabrownin was best in presenting 6 obvious strips in column, with difference of their chromatism parameter values, total reducing power, pH value, conventional component, UV and IR spectroscopy scanning, and stability etc. The study further demonstrated complexity of compositions and properties of theabrownin. At the same time, this investigation laid a foundation for the further research of bioactivities and chemical essence of theabrownin.
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